Quantitate nuclear images for clinical diagnosis

New insights into cell cycle and DNA damage response machineries through high‐resolution AMICO quantitative imaging cytometry

“…To expand capabilities of quantitative confocal microscopy in terms of analysis of proximity of sites of DNA damage induced by anticancer drugs vis-á-vis DNA replication sites (“replication factories”) further approaches have been recently developed utilizing computerized algorithms that allow to measure spatial immediacy of these sites in 3D space (16, 17). By applying quantitative nuclear imaging even clinical diagnosis is at reach (18). All the above technologies have a fair to excellent spatial resolution but are relatively limited in their ability to combine the quantitation of multiple markers on individual cells in a single run.…”