Quantitation and detection of isotypes of ANTI‐SS‐B antibodies by elisa and farr assays using affinity purified antigens

Venables, P. J. W.; Charles, Peter; Buchanan, Russell; Yi, Tung; Mumford, P. A.; Schrieber, Leslie; Room, G; Maini, R. N.

doi:10.1002/art.1780260205

Cited by 81 publications

(14 citation statements)

References 21 publications

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“…Based on the present results, an increase in this autoantibody should be associated with an increase in salivary gland inflammation. A previous longitudinal study of a patient with SS found that an increase in serum anti-SS-B/La was associated with parotid gland swelling and the development of purpura (11 Previously, a correlation was found between total serum IgG and anti-SS-B/La values, but no relationship was demonstrated between levels of anti-SS-B/La and focus scores (8). This lack of correlation might have been the result of an unequal distribution of focus scores in the patient group studied.…”

Section: Resultsmentioning

confidence: 98%

Serum anti—SS‐B/La and IgA rheumatoid factor are markers of salivary gland disease activity in primary sjögren's syndrome

Atkinson

Travis

Slocum

et al. 1992

Arthritis & Rheumatism

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Objective. To identify serologic markers of salivary gland disease activity in 43 patients with primary Sjögren's syndrome.Methods. Comparison of salivary gland biopsies (focus scores) and flow rates with serum concentrations of IgA and IgM rheumatoid factor (RF), total serum IgG, serum anti—SS‐B/La antibodies, and the erythrocyte sedimentation rate.Results. Serum anti—SS‐B/La antibody levels correlated with focus scores (rs = 0.477, P < 0.0025). Serum IgA‐RF concentrations correlated inversely with stimulated parotid gland salivary flow rates (rs = −0.394, P < 0.01).Conclusion. Measuring serum levels of anti—SS‐B/La and IgA‐RF would be useful when monitoring salivary responses in therapeutic trials, especially in patients with minimal salivary function.

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Section: Resultsmentioning

confidence: 98%

Serum anti—SS‐B/La and IgA rheumatoid factor are markers of salivary gland disease activity in primary sjögren's syndrome

Atkinson

Travis

Slocum

et al. 1992

Arthritis & Rheumatism

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“…Thus, MHC control of these immune responses, either qualitatively or quantitatively, has been suggested. Recently, enzyme-linked immunosorbent assays (ELISA) have been developed to measure anti-Ro, anti-La, and the anti-Sdnuclear RNP (anti-SmhRNP) complex (14)(15)(16)(17)(18)(19)(20). By virtue of being more sensitive and quantitative, these assays may provide greater insight into the role…”

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confidence: 99%

Two ro (ss‐a) autoantibody responses in systemic lupus erythematosus

Hamilton

Harley

Bias

et al. 1988

Arthritis & Rheumatism

176

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Sdnuclear RNP were quantitated by enzyme-linked immunosorbent assay in 106 white patients with systemic lupus erythematosus. Two Ro autoantibody subgroups were identified that differed quantitatively, genetically, and clinically. The subgroup having anti-Ro only demonstrated significantly lower mean anti-Ro levels than did the subgroup with concomitant anti-La and showed a strong association with the linked HLA alleles DR2 and DQwl. The anti-Ro with anti-La sub- group was associated with the linked HLA alleles B8, DR3, DRw52, and DQw2 (DR3 was primary), and this subgroup consisted of patients with older ages at disease onset, sicca complex, and less renal involvement. Overall, the relative risk (RR) for having anti-Ro was highest in HLA-DR21DR3 heterozygotes compared with non-DR2/DR3 heterozygotes (RR 15) and all other DR combinations (RR 7), suggesting a compound effect of 2 immune responses. Heterozygotes for HLA-DQwl/ DQw2 demonstrated significantly higher mean levels of anti-Ro, which may be indicative of trans gene interaction at HLA-DQ. These data suggest the hypothesis that HLA genes exert their major effects on Ro/La autoantibody subsets of systemic lupus erythematosus.Genetic predisposition to systemic lupus erythematosus (SLE) has been associated with several alleles of the major histocompatibility complex (MHC). Those most consistently reported include HLA-B8, DR2, DR3, and DQwl, as well as null alleles of the HLA-linked second and fourth components of complement (1-8). When autoantibody subsets of SLE are considered, certain HLA antigen correlations become stronger. Most notably, in patients with SLE and in patients with other autoimmune diseases expressing anti-Ro and anti-La, HLA-DR3 andlor DR2 are found in the majority (4,5,9-13). Thus, MHC control of these immune responses, either qualitatively or quantitatively, has been suggested. Recently, enzyme-linked immunosorbent assays (ELISA) have been developed to measure anti-Ro, anti-La, and the anti-Sdnuclear RNP (anti-SmhRNP) complex (14)(15)(16)(17)(18)(19)(20). By virtue of being more sensitive and quantitative, these assays may provide greater insight into the role

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“…These proteins are likely to be components of small nuclear ribonucleoprotein (snRNP) particles, because sera B. M. and P.S. contain anti-RNP and antiSm antibodies (Table 1), which bind, respectively, to the 68-and 16-kDa polypeptides found within these particles (17)(18)(19), and serum I. L. contains anti-La antibodies, which are specific for a 53-kDa ribonucleoprotein (20)(21)(22).…”

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confidence: 99%

Antibodies to histones in systemic lupus erythematosus: localization of prominent autoantigens on histones H1 and H2B.

Hardin¹,

Thomas²

1983

Proc. Natl. Acad. Sci. U.S.A.

128

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By the technique of immunoblotting we have assessed the ability of sera from 24 patients with systemic lupus erythematosus to bind nuclear proteins. Of the 11 patients who had antibodies to histones, 10 had antibodies to histone HI and 9 of these also had antibodies to histone H2B. Antibodies to the other histones (H2A, H3, and H4) were less apparent. Five of the 11 patients (and two others in the remainder of the sample of 24) also had antibodies to a small number of nonhistone proteins that are probably components of ribonucleoprotein particles, but there was no obvious correlation between the presence of antihistone antibodies and the known antiribonucleoprotein activity of these sera.Separate determinants on HI and H2B were demonstrated by immunoblotting with affinity-purified anti-HI and anti-H2B antibodies derived from serum that showed both specificities. The localization of the determinants within the histone polypeptide chains was shown by immunoblotting with large fragments produced by specific proteolytic or chemical cleavage of the histones. The strongest determinant on HI was located within the COOH-terminal half, with a weaker determinant being present within the NH2-terminal half; the H2B determinant(s) was located entirely within the NH2-terminal half of the molecule. The selectivity with which the antihistone antibodies in systemic lupus erythematosus are produced against the more exposed histones in the nucleosome (and perhaps against the most exposed regions of these histones) is consistent with the involvement of intact chromatin structures as immunogens in this disease.Antinuclear antibodies (ANAs) can be classified conveniently into groups directed against (i) ribonucleoproteins (RNP), (ii) deoxyribonucleoprotein, (iii) naked nucleic acids, and (iv) other constituents of the nucleus such as the nuclear matrix or enzymes. Recently, it has become clear that patients with systemic lupus erythematosus (SLE) produce at least nine different autoantibodies that recognize selected sets of RNP (1-3). Specificity within this system is based on the ability of the antibodies to identify proteins that bind with great selectivity to particular RNA molecules. These studies suggested that antibodies to deoxyribonucleoprotein might follow a similar pattern and represent a broad spectrum of specificities for different proteins that complex with DNA. In the present study, we have used the immunoblotting technique (4) to analyze the ability of antibodies in SLE sera to recognize nuclear protein antigens. The results indicate that the most prominent antigenic determinants of chromatin are located on histones, particularly HI and H2B, within regions that are likely to be exposed in the structure of native chromatin. MATERIALS AND METHODSPatients. We have studied 24 patients who fulfilled the American Rheumatism Association criteria for SLE (5). All had ANAs demonstrable by indirect immunofluorescence. The sera were stored at -70'C until assayed and transported from New Haven to Cambridge, England, in so...

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Quantitation and detection of isotypes of ANTI‐SS‐B antibodies by elisa and farr assays using affinity purified antigens

Cited by 81 publications

References 21 publications

Serum anti—SS‐B/La and IgA rheumatoid factor are markers of salivary gland disease activity in primary sjögren's syndrome

Serum anti—SS‐B/La and IgA rheumatoid factor are markers of salivary gland disease activity in primary sjögren's syndrome

Two ro (ss‐a) autoantibody responses in systemic lupus erythematosus

Antibodies to histones in systemic lupus erythematosus: localization of prominent autoantigens on histones H1 and H2B.

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