Quantitation and identification of human monocytic colony-stimulating factor in human serum by enzyme-linked immunosorbent assay

Hanamura, T; Motoyoshi, Kazuo; Yoshida, Katsuo; Saito, Minoru; Miura, Yasuo; Kawashima, T; Nishida, Masayuki; Takaku, F

doi:10.1182/blood.v72.3.886.bloodjournal723886

Cited by 95 publications

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“…The M‐CSF level was determined by the sandwich enzyme‐linked immunosorbent assay (ELISA) method using three antibodies: equine antihuman M‐CSF antibody coated on a microtiter plate, an antibody solution containing highly purified rabbit antihuman M‐CSF antibody, and caprine enzyme‐conjugated antirabbit immunoglobulin G (IgG) antibody, according to the method reported by Hanamura and colleagues 16 . TP level was measured with a pyrogallol red‐molybdate complex reaction using a Micro TP‐AR Kit (Wako Pure Chemical Industries Ltd, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Increased Levels of Macrophage Colony‐Stimulating Factor in the Placenta and Blood in Preeclampsia

Hayashi

Hoshimoto

Ohkura

et al. 2002

American J Rep Immunol

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Section: Methodsmentioning

confidence: 99%

Increased Levels of Macrophage Colony‐Stimulating Factor in the Placenta and Blood in Preeclampsia

Hayashi

Hoshimoto

Ohkura

et al. 2002

American J Rep Immunol

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“…The concentration of M‐CSF was measured by an radioimmunological method after raising anti‐M‐CSF antibodies in New Zealand rabbits 22−24 . Previous in‐house validation experiments had confirmed that non‐specific binding antibodies were less than 5% of the total population.…”

Section: Methodsmentioning

confidence: 99%

Disrupted mucosal barrier in quiescent ulcerative colitis: effect of metronidazole and of a symbiotic preparation in a pilot cross‐over study

Marotta

Naito²,

Tajiri

et al. 2003

Chinese Journal of Digestive Diseases

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OBJECTIVES: The aims of the present study were to investigate the plasma concentration of endotoxin in patients with inflammatory bowel disease (IBD), the effect of metronidazole (MNZ) and of symbiotics on that concentration, and the relationship to IBD activity. METHODS: The study group comprised 26 patients with quiescent ulcerative colitis (UC), all of whom were on maintenance mesalazine treatment (1200− 2400 mg/day). The control group comprised 15 subjects. Blood samples were taken from all study subjects to measure: routine blood chemistry, endotoxin concentration, lipopolysaccharide binding protein (LBP) and macrophage‐colony stimulating factor (M‐CSF). All the IBD patients were randomly enrolled for a 2‐week oral daily treatment regimen with either MNZ (250 mg t.i.d.) or the symbiotic mixture SCM‐III (Lactobacillus acidophilus, L. helveticus and Bifidobacteria brevis in an ion‐ and vitamin‐enriched medium; 3 mL t.i.d.). Following a 6‐week washout period during which the patients continued their maintenance treatment, the cross‐over study of the new treatments was begun. Blood parameters were checked at entry and 2 weeks after each treatment schedule. RESULTS: The concentration of endotoxin level in the UC patients, as a whole, was comparable with that of the control subjects. However, a separate group of patients with long‐standing disease and pancolitis showed a statistically significant increase in toxin. SCM‐III, but not MNZ, normalized this parameter. There was no statistical change in LBP and plasma endotoxin‐inhibiting capacity in the IBD patients. The M‐CSF concentration was increased in the UC group, particularly in the pancolitis subgroup. SCM‐III, but not MNZ, significantly decreased the M‐CSF concentration in the UC patients, but there was only an insignificant trend toward decrease in the subgroup. There was a significant correlation between M‐CSF and endotoxin in the pancolitis subgroup (r: 0.74, P < 0.05). CONCLUSIONS: Although these preliminary results need to be treated with caution, they suggest the effectiveness of long‐term administration of probiotics/symbiotics in conjunction with standard treatment in patients with UC, even if there is not gross disease activity.

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“…The M−CSF level was determined using the sandwich ELISA meth− od with three antibodies ± equine anti−human M−CSF antibody coated on a microtiter plate, an antibody solution containing highly purified rabbit antihuman M−CSF antibody, and caprine enzyme−conjugated anti−rabbit IgG antibody according to the method reported by Hanamura et al [18]. These antibodies origi− nated from the laboratory where Hanamura et al [18] worked.…”

Section: Determination Of M−csfmentioning

confidence: 99%

Serum Levels of Macrophage Colony-stimulating Factor in Singleton and Twin Pregnancy

Hayashi¹,

Fukasawa²,

Inaba³

2006

Horm Metab Res

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Macrophage colony-stimulating factor (M-CSF) is present in the placenta and has been implicated in placental growth and development. M-CSF levels in peripheral blood increase significantly with progression of pregnancy in uncomplicated singleton pregnancy. This study investigated whether serum M-CSF levels in singleton pregnancy differed from those in twin pregnancy. A hundred and four pregnant women, of whom fifty-nine were women carrying single fetuses and forty-five were women with twin fetuses, participated in the study. Their average gestational age at entry was 10, 20, 30, and 38 weeks of gestation in singleton pregnancy and 10, 20, 30, and 35 weeks in twin pregnancy. Peripheral blood was collected and serum was separated after centrifugation and stored at - 20 degrees C. M-CSF levels were determined by ELISA. In both groups, the serum levels of M-CSF increased significantly as pregnancy progressed. M-CSF levels were relatively higher in twin pregnancy than in singleton pregnancy at 10, 20, and 30 weeks. At 35 and 38 weeks, the M-CSF levels were significantly higher in twin pregnancy than in singleton pregnancy. Elevation of serum M-CSF supports M-CSF production in the placenta. This elevation in twin pregnancy may be related to increased demand of M-CSF in twin pregnancy.

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Quantitation and identification of human monocytic colony-stimulating factor in human serum by enzyme-linked immunosorbent assay

Cited by 95 publications

References 0 publications

Increased Levels of Macrophage Colony‐Stimulating Factor in the Placenta and Blood in Preeclampsia

Increased Levels of Macrophage Colony‐Stimulating Factor in the Placenta and Blood in Preeclampsia

Disrupted mucosal barrier in quiescent ulcerative colitis: effect of metronidazole and of a symbiotic preparation in a pilot cross‐over study

Serum Levels of Macrophage Colony-stimulating Factor in Singleton and Twin Pregnancy

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