Quantitation by HPLC-UV of Mangiferin and Isomangiferin in Coffee (Coffea arabica) Leaves from Brazil and Costa Rica After Solvent Extraction and Infusion

Trevisan, M.; Almeida, Ricardo Farias de; Soto, Gabriela; Filho, Elías de Melo Virginio; Ulrich, Cornelia M.; Owen, Robert W.

doi:10.1007/s12161-016-0457-y

Cited by 28 publications

(26 citation statements)

References 20 publications

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“…The use of the diode array detector (DAD) coupled to the UHPLC ensures the clear identification of each component. HPLC coupled with a DAD is the most reported method for the quantification of the main secondary metabolites present in coffee leaves [11][12][13], in which each run averaging over 25 min. The use of UHPLC-DAD in this work has allowed to complete the analysis of each run in 14 min, considerably improving the length of time require for this analysis, and consequently decreasing the limit of detection.…”

Section: Validation Of the Uhplc-dad Methodsmentioning

confidence: 99%

“…However, literature data on the quantification of main secondary metabolites present in coffee leaves do not often provide this information. To the best of our knowledge only one study has reported values of LOD and LOQ when quantifying mangiferin in coffee leaves [12].…”

Section: Validation Of the Uhplc-dad Methodsmentioning

confidence: 99%

“…For this reason, the study of the chemical composition of coffee leaves, from which tea is made, is now a priority. A number of studies have been done to identify bioactive compounds present in coffee leaves [10][11][12][13]. The results identified three main classes of bioactive compounds, namely alkaloids, chlorogenic acids and more recently xanthones [13].…”

Section: Introductionmentioning

confidence: 99%

“…Coffee leaves have been found to contain an amount of mangiferin comparable to M. indica leaves [20]. Recently, the accumulation of mangiferin has been demonstrated in the leaves of several coffee species, suggesting that this maybe an excellent natural source of this xanthone [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Antioxidants in Coffee Leaves: Impact of Botanical Origin and Maturity on Chlorogenic Acids and Xanthones

Monteiro

Colomban²,

Azinheira

et al. 2019

Antioxidants

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Natural polyphenols are important dietary antioxidants that significantly benefit human health. Coffee and tea have been shown to largely contribute to the dietary intake of these antioxidants in several populations. More recently, the use of coffee leaves to produce tea has become a potential commercial target, therefore prompting studies on the quantification of polyphenols in coffee leaves. In this study a variety of coffee leaf species, at different development stages, were analyzed using ultra-high pressure liquid chromatography. The results demonstrate that both the botanical origin of the samples and their maturity influence significantly the concentration of the antioxidants; for total chlorogenic acids a two-fold difference was found between different species and up to a three-fold variation was observed between young and mature leaves. Furthermore, the range of concentrations of chlorogenic acids in young leaves (35.7–80.8 mg/g of dry matter) were found to be comparable to the one reported for green coffee beans. The results provide important data from which potential new commercial products can be developed.

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Section: Validation Of the Uhplc-dad Methodsmentioning

confidence: 99%

Section: Validation Of the Uhplc-dad Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary Antioxidants in Coffee Leaves: Impact of Botanical Origin and Maturity on Chlorogenic Acids and Xanthones

Monteiro

Colomban²,

Azinheira

et al. 2019

Antioxidants

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show abstract

“…The HPLC methods already reported in the literature for determination of Mgf were tried with reasonable modifications, so as to obtain good peak shapes without tailing effects (Fenglei et al, ; Trevisan et al, ). Finally, the method reported by Wang et al () was selected with little modification because of good peak symmetry and good separation between the degradation products as well as between the degradation products.…”

Section: Methodsmentioning

confidence: 99%

Elucidation of stress‐induced degradation products of mangiferin: Method development and validation

Khurana

Kaur²,

Kaur³

et al. 2017

Biomedical Chromatography

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The degradation behavior of mangiferin, under various ICH Q1A(R2) recommended stress conditions, was studied using an isocratic elution with mobile phase (pH 2.4), composed of acetonitrile and 1% orthophosphoric acid (12:88 v/v) at a flow rate of 1.0 mL/min, with λ 262 nm. It was suitably adapted for LC-MS studies by replacing with 1% acetic acid (ACN-1% acetic acid; 18:82) and the pH was adjusted to 3.0. Extensive degradation was found to occur during alkaline medium stress studies at 2.31 min of retention time at λ of 235 nm. The mass spectrum of mangiferin, 3 h after treatment with 0.1 M NaOH, clearly shows the rupture of the tricyclic ring, indicating that a fragment at m/z - 269 was formed. Furthermore, the results were supported by nuclear magnetic resonance as well. However, no degradation was observed in other stress conditions.

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Application of a Quality by Design‐based approach in development and validation of an RP‐HPLC method for simultaneous estimation of methotrexate and mangiferin in dual drug‐loaded liposomes

Adin

Gupta

Aqil

et al. 2023

Biomedical Chromatography

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The present study delineates the development of a novel rugged and sensitive stability-indicating risk-based HPLC method for the concurrent estimation of methotrexate and mangiferin in dual drug-loaded nanopharmaceuticals based on an analytical QbD approach. Preliminary screening trials along with systemic risk analysis were performed, endeavouring to explicate the critical method attributes, namely pH, percentage orthophosphoric acid content and percentage methanol content, that influence critical quality attributes. Box-Behnken design was utilized for the optimization of the tailing factor as response for methotrexate and mangiferin in short run time.The chromatographic conditions were optimized by performing 17 experimental runs acquired from Design-Expert software. The chromatographic conditions after the analysis of an optimized zone within the confines of the design space were chosen as mobile phase water-methanol adjusted to pH 3.0 with 0.05% orthophosphoric acid (65:35, v/v) and flow rate 1.0 ml/min using a C 18 analytical column at an isosbestic wavelength of 265 nm. Furthermore, the validation of the optimized method was done in accordance with International Conference on Harmonization guidelines and were reckoned to be in the prescribed limits. The developed RP-HPLC method has a high degree of practical utility for synchronous detection of methotrexate and mangiferin in pharmaceutical nano-dosage forms such as protein-based-nanoparticles, nanocrystals, polymeric nanoparticles and metallic nanoparticles in in vivo and in vitro studies.

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Quantitation by HPLC-UV of Mangiferin and Isomangiferin in Coffee (Coffea arabica) Leaves from Brazil and Costa Rica After Solvent Extraction and Infusion

Cited by 28 publications

References 20 publications

Dietary Antioxidants in Coffee Leaves: Impact of Botanical Origin and Maturity on Chlorogenic Acids and Xanthones

Dietary Antioxidants in Coffee Leaves: Impact of Botanical Origin and Maturity on Chlorogenic Acids and Xanthones

Elucidation of stress‐induced degradation products of mangiferin: Method development and validation

Application of a Quality by Design‐based approach in development and validation of an RP‐HPLC method for simultaneous estimation of methotrexate and mangiferin in dual drug‐loaded liposomes

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