Quantitation of Acetazolamide in Plasma by High-Performance Liquid Chromatography

Pharmacokinetics of acetazolamide after intravenous and oral administration in horses

“…Acetazolamide assay-Plasma acetazolamide concentrations were determined by HPLC after extraction of acetazolamide with ethyl acetate, using modifications of a reported assay. 16 Briefly, 50 µl of internal standard (20 µg of chlorothiazide f /ml of deionized H 2 O), 0.5 ml of 0.05 M sodium acetate buffer, and 10 ml of ethyl acetate were added to 1 ml of sample in a glass culture tube. g The tube was capped, and the combination was vortexed for 3 minutes, then centrifuged at 1,000 X g for 10 minutes.…”

“…In humans, acetazolamide is absorbed rapidly and undergoes biphasic elimination. 16,17 Limited pharmacokinetic data derived from a study of the effects of acetazolamide on exerciseinduced metabolic and respiratory responses in horses revealed a mean unbound plasma acetazolamide concentration of 1.38 ± 0.64 µg/ml approximately 14 hours after 3 consecutive days of oral administration (30 mg/kg of body weight, q 12 h). 18 The purposes of the study reported here were to develop a suitable high-performance liquid chromatography (HPLC) assay for measuring acetazolamide plasma concentrations and to use this assay to study the pharmacokinetics of acetazolamide after IV and oral administration in horses.…”

Tb -4'carboxybenzo-18crown-6-ether Photo Probe or the Assessment of Nalbuphin HCl in Serum and Pharmaceutical Formulations

“…Luminescent photo probes lanthanide complexes have more advantages over the present ones; photo probe has high stability and durability. The photo probe can provide constant signal response for 2 years which is 24-fold better stability compared to the life time warranted for the chromatographic and colorimetric methods [6][7][8][9][10][11][12][13][14]. Photo probe is stable over all measurements which prevent the source of error in the measurement process and it gives a low standard deviation values.…”

LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application

“…The etiology of many of the adverse effects is unclear, may be due to acidosis or carbon dioxide retention. 3 After oral administration, the peak blood levels were attained in 4-8 h with elimination half-life (t 1/2 ) about 10-15 h. 1,2 Based on the literature, many HPLC based analytical methods have been reported for the determination of acetazolamide in human plasma, [4][5][6] rat plasma and tissues, 7 human urine 8 and in dosage forms. 9 The conventional HPLC methods must sacrifice time, resolution or sensitivity.…”