Quantitation of chromosome 1p and 19q deletions in glial tumours by interphase FISH on formalin-fixed paraffin-embedded tissue

Gonzales, Michael; Dale, Susan; Susman, Marleen; Mills, Joseph L.

doi:10.1016/j.jocn.2005.06.004

Cited by 11 publications

(10 citation statements)

References 26 publications

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“…Eleven cases have been investigated for the loss of heterozygosity of chromosome arms 1p and 19q using fluorescence in situ hybridization (FISH) technique [6]. Duplicate 5 µm paraffin wax sections were treated following supplier instructions (FISH Accessory Kit, Dako, Cambs, UK and Vysis LSI DNA Probes, Abbott Diagnostics GmbH, Wiesbaden, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Sections of 7 mm thickness (14 mm for LFB/N) from paraffin-embedded blocks were dewaxed in xylene and rehydrated through graded alcohols. Endogenous peroxidase was blocked using 1% hydrogen peroxide in metha- Eleven cases have been investigated for the loss of heterozygosity of chromosome arms 1p and 19q using fluorescence in situ hybridization (FISH) technique [6]. Duplicate 5 mm paraffin wax sections were treated following supplier instructions (FISH Accessory Kit, Dako, Cambs, UK and Vysis LSI DNA Probes, Abbott Diagnostics GmbH, Wiesbaden, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Diffuse form of dysembryoplastic neuroepithelial tumour: the histological and immunohistochemical features of a distinct entity showing transition to dysembryoplastic neuroepithelial tumour and ganglioglioma

Bódi¹,

Selway²,

Bannister³

et al. 2012

Neuropathology Appl Neurobio

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Diffuse form of dysembryoplastic neuroepithelial tumour: the histological and immunohistochemical features of a distinct entity showing transition to dysembryoplastic neuroepithelial tumour and ganglioglioma

Bódi¹,

Selway²,

Bannister³

et al. 2012

Neuropathology Appl Neurobio

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“…Several molecular techniques are described in the literature to study the chromosomal status of tumor cells, including fluorescence in situ hybridization (FISH) [22], [23], [24], [25], [26], polymerase chain reaction [27], [28], quantitative microsatellite analysis [14], [29], loss of heterozygosity (LOH) by microsatellite analysis [8], [10], [13], [30], [31], [32], [33], [34], [35], comparative genomic hybridization array (CGH) [17], [36], [37], [38], [39], [40], [41] and multiplex ligation - dependent probe [42]. All these techniques have their advantages and disadvantages but the most widely used among them is FISH [26] because it can be performed by fluorescent microscopy on paraffin embedded tumor tissue sections and is thus easily accessible to most pathology laboratories.…”

Section: Introductionmentioning

confidence: 99%

ImmunoFISH Is a Reliable Technique for the Assessment of 1p and 19q Status in Oligodendrogliomas

et al. 2014

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ObjectiveTo develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.MethodsValidation was performed by two observers on a series of 36 pre-selected oligodendrogliomas and compared to the results previously determined by FISH alone.ResultsThe ImFISH technique is easy to perform and to analyze and is no more time-consuming than the usual FISH technique. Our results show that the inter-observer reliability of ImFISH is high (κ = 0.86 and 0.95 respectively for 1p and 19q). Compared to FISH, the ImFISH exhibits a very high sensitivity (∼100%) and specificity (∼90%) for 1p and/or 19q deleted cases. The sensitivity is high for normal cases (∼85%) and imbalanced cases (∼90%) with a specificity ranging between 50 and 85%. Finally, there were no significant differences between FISH and ImFISH results calculated on 60, 40 or 20 cells.ConclusionOur study demonstrates the reliability of the ImFISH technique in oligodendrogliomas and emphasizes its advantage in poorly cellular tumoral specimen.

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“…Deparaffinized sections of all 14 initial and four re‐operated lesions were analyzed for chromosome 1p and 19q deletions by a previously published FISH protocol 6 …”

Section: Methodsmentioning

confidence: 99%

Dysembryoplastic neuroepithelial tumor (DNT)‐like oligodendrogliomas or DNTs evolving into oligodendrogliomas: Two illustrative cases

et al. 2007

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A review of dysembryoplastic neuroepithelial tumors (DNTs) in 14 patients over a 12-year period revealed four patients re-operated because of changes on magnetic resonance imaging (MRI) suggesting tumor recurrence or progression. In three of these, the histological features were identical to the initial DNT. In the fourth patient, persistent DNT was surrounded by WHO grade 2 oligoastrocytoma. In one of the other 10 patients, WHO grade 2 oligodendroglioma was present in white matter deep to and completely separate from a cortically based DNT. Fluorescence in situ hybridization showed codeletion of 1p and 19q in both the DNT and oligodendroglioma and oligoastrocytoma components. Deletions were not identified in any other tumor. Our findings corroborate other studies that 1p and 19q deletions are uncommon in DNT. These two unusual tumors also raise the possibility that rare DNTs may evolve into oligodendroglioma or oligoastrocytoma. DNTs with this altered biology can be identified by 1p and 19q deletion analysis.

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Quantitation of chromosome 1p and 19q deletions in glial tumours by interphase FISH on formalin-fixed paraffin-embedded tissue

Cited by 11 publications

References 26 publications

Diffuse form of dysembryoplastic neuroepithelial tumour: the histological and immunohistochemical features of a distinct entity showing transition to dysembryoplastic neuroepithelial tumour and ganglioglioma

Diffuse form of dysembryoplastic neuroepithelial tumour: the histological and immunohistochemical features of a distinct entity showing transition to dysembryoplastic neuroepithelial tumour and ganglioglioma

ImmunoFISH Is a Reliable Technique for the Assessment of 1p and 19q Status in Oligodendrogliomas

Dysembryoplastic neuroepithelial tumor (DNT)‐like oligodendrogliomas or DNTs evolving into oligodendrogliomas: Two illustrative cases

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