Quantitation of DNA Adducts of Aristolochic Acids in Repair-Deficient Cells: A Mechanistic Study of the DNA Repair Mechanism

Abstract

Accumulating evidence has revealed that nephrotoxic and carcinogenic aristolochic acids (AAs) released from decaying Aristolochia clematitis L. weeds are soil and food grain contaminants in the Balkan Peninsula, while AA toxicity has been linked to induced DNA damage. In this study, we investigated the DNA repair mechanism that excises the aristolactam-DNA adducts in gene-knockout Escherichia coli cells. These results demonstrated that cell lines deficient in nucleotide excision repair (NER) machinery accumulated higher adduct levels, indicating that NER is the major mechanism responsible for the repair of these lesions. Furthermore, data also revealed the involvement of base excision repair enzymes in repairing the lesions but with lower contribution than NER.

Analysis of aristolochic acids in Houttuynia cordata by liquid chromatography–tandem mass spectrometry

“…To fully address public concern over the safety of using H. cordata as a food ingredient and an herbal remedy, highly sensitive and selective analytical methods are needed to detect AAs in the complex matrix of the plant extract. LC–MS/MS is a highly sensitive and selective analytical technique that has been used for a wide variety of applications, such as toxicology, environmental, and food contaminant analyses 41–46 . Our assay offers MDLs at least 1700 times lower than the HPLC–UV method and thus was used to study whether AA‐I and AA‐II naturally exist in H. cordata .…”

“…LC-MS/MS is a highly sensitive and selective analytical technique that has been used for a wide variety of applications, such as toxicology, environmental, and food contaminant analyses. [41][42][43][44][45][46] Our assay offers MDLs at least 1700 times lower than the HPLC-UV method and thus was used to study whether AA-I and AA-II naturally exist in H. cordata.…”

Determination of Aristolochic Acid Using Isocratic RP-HPLC Method

“…These patients reported the presence of AA-derived DNA adducts in their kidneys and ureteric tissues, confirming AAs as the primary cause of AAN. These patients reported finding AA-derived DNA adducts in their kidneys and ureteric tissues, suggesting AAs as the primary cause of AAN [6] . If patients consume products suspected of containing AAs for an extended time, they will be at a higher risk of nephrotoxicity.…”

Dual‐spectroscopic real‐time monitoring of the reduction reaction between aristolochic acid I and Fe2+ and its bio‐application

“…Raman spectroscopy is a nondestructive sensitive technique that can be carried out for in situ and in vivo analysis, thereby making it one of the most widely used analytical techniques. [ 1–6 ] It is generally believed that the signal of the target analyte in surface‐enhanced Raman spectroscopy (SERS) is amplified because of the electromagnetic field interaction between light and the metal substrate, which generates a larger laser field through excitation usually known as plasmon resonance. The analyte is detected by local surface plasmon resonance, and the Raman intensity is increased, thereby increasing the detection sensitivity.…”

“…NRS, SERS data and vibrational assignment of AAI on Au@Ag NPs Table S2. AAI infrared peak attribution [ 1 ] Figure S2. Linearity of AAI at 20‐100 μM in SERS.…”