Quantitation of endotoxin by gas chromatography-mass spectrometry in Neisseria meningitidis serogroups A, C, W, Y and X during polysaccharide purification used in conjugate vaccine

Shende, Niraj; Karale, Abhijeet; Marne, Kishor; Deshpande, Hrishikesh; Belapurkar, Hrushikesh; Mallya, Asha D.; Dhere, Rajeev

doi:10.1016/j.jpba.2021.114536

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…For this purpose, various methods, including gas chromatography (GC) and liquid chromatography (LC) or biosensors, are used for their quantification. GC is a popular method in the quantification of endotoxins, but its limitation is the need for chemical derivatization such as transesterification or acetylation of the compounds in tested samples to obtain volatile derivatives or analogues 11,12 . LC, especially when coupled with MS, allows the measurement of very low levels of endotoxins without the need to modify the samples; however, the analysis itself takes quite a long time and requires the consumption of large amounts of reagents 9 .…”

Section: Introductionmentioning

confidence: 99%

Infrared pulsed fiber laser‐produced silver‐109 nanoparticles for laser desorption/ionization mass spectrometry of 3‐hydroxycarboxylic acids

Kołodziej

Płaza‐Altamer

Nizioł

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale3‐Hydroxycarboxylic acids are one of the major components of bacterial lipopolysaccharides (LPS), also known as endotoxins. Endotoxins pose a serious health risk and can seriously damage the internal organs of humans and animals. 3‐Hydroxycarboxylic acids can be used as environmental markers to determine endotoxin levels. At the time of preparation of this manuscript no studies on laser mass spectrometry (MS) and analysis with silver nanoparticles (NP) for 3‐hydroxycarboxylic acids have been published in literature.MethodsSix acids, 3‐hydroxyoctanoic (3‐OH‐C8:0), 3‐hydroxydecanoic (3‐OH‐C10:0), 3‐hydroxydodecanoic (3‐OH‐C12:0), 3‐hydroxytetradecanoic (3‐OH‐C14:0), 3‐hydroxyhexadecanoic (3‐OH‐C16:0), and 3‐hydroxyoctadecanoic (3‐OH‐C18:0) acids, were used as test compounds on the target containing silver‐109 NPs for quantification using matrix‐assisted laser desorption/ionization (MALDI)–type mass spectrometer. Methods were also tested on spiked human blood serum samples to quantify 3‐hydroxycarboxylic acids and verify the influence of the biological matrix on the measurement.ResultsAnalyzed acids were directly tested in 1 000 000‐fold concentration change conditions ranging from 1 mg/mL to 1 ng/mL. The semi‐automatic MSI (MS imaging) method allowed us to obtain two to five times lower limit of detection (LOD) and lower limit of quantitation (LLOQ) values than common LDI (Bruker Daltonics, Bremen, Germany) method for analyzed acids. For almost all results of 3‐hydroxycarboxylic acids, the trendline fit was better for the semi‐automatic MSI method than the manual LDI method.ConclusionFor the first time, the use of laser MS for the quantification of 3‐hydroxycarboxylic acids has been demonstrated, and it has been proven that it can be used in the quantitative analysis of such compounds over a wide range of concentrations. In addition, a comparison of two methods—manual LDI‐MS and semi‐automatic MSI—is presented.

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Section: Introductionmentioning

confidence: 99%