Quantitation of glutathione S transferase‐π in the urine of preterm neonates

Tsukahara, Hirokazu; Toyo-Oka, Michiyo; Kanaya, Yuko; Ogura, Kazumasa; Kawatani, Masao; Hata, Atsuko; Hiraoka, Masahiro; Mayumi, Mitsufumi

doi:10.1111/j.1442-200x.2005.02123.x

Cited by 13 publications

(19 citation statements)

References 19 publications

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“…In the newborn period oxydative damage has been implicated in the pathogenesis of numerous diseases and entities, such as maternal preeclampsia (4), pre mature birth (5-7), asphyxia (8,9), neonatal respira tory distress syndrome (10)(11)(12), retinopathy (7,10).…”

Section: Oxidative Stress In Pediatric Diseasesmentioning

confidence: 99%

Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

Bajcetic¹,

Brajovic²,

Korkut-Tešić³

2010

Journal of Medical Biochemistry

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Kratak sadr`aj: Farmakoterapija oboljenja kod dece predstavlja veliki izazov s obzirom da najve}i broj lekova koji se svakodnevno koristi nije pedijatrijski evalurian. Efikasnost terapije zavisi u velikoj meri od poznavanja patofiziolo{kih procesa u organizmu dece razli~itih uzrasta te istra`ivanja u tom pravcu predstavljaju imperativ. Naru{en balans u produkciji slobodnih kiseoni~kih/azotnih vrsta i parametara antioksidantne za{tite zna~ajan je patofiziolo{ki ~inilac brojnih oboljenja (npr. sr~ana insuficijencija, plu}na hiper tenzija, astma, neonatalna sepsa, karcinom i dr.) u razli~i tim de~ijim uzrastnim periodima. Reaktivne kiseoni ~ne/azot ne vrste imaju funkciju signalnih molekula u normal nim fiziolo{kim procesima. Njihova pove}ana produkcija mo`e izazvati o{te}enja koja mogu naru{iti normalne fiziolo{ke procese u }eliji i u krajnjem ishodu izazvati }elijsku smrt. U ovom radu dat je pregledni prikaz dosada{njih ispitivanja parametara oksidativnog stresa kod dece razli~ite starosne dobi za pojedina oboljenja. Tako|e, razmotrili smo sve potencijalne dijagnosti~ke i terapijske mogu}nosti parametara oksidativnog stesa u de~ijem uzrastu.Klju~ne re~i: parametri oksidativnog stresa, antioksidansi, deca.

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Section: Oxidative Stress In Pediatric Diseasesmentioning

confidence: 99%

Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

Bajcetic¹,

Brajovic²,

Korkut-Tešić³

2010

Journal of Medical Biochemistry

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“…2), the urinary 8-OHdG level increased during the first 1 month of life in two previous studies [13,20], but not in two other reports [14,17]; the urinary 8-OHdG levels at 14 and 28 days of age were significantly lower or similar to those determined within 7 days of age among breast-or formula-fed neonates, respectively [17], and in another study on 20 full-term neonates by Dziaman et al [14], the urinary 8-OHdG level continued to increase until the age of 14 days and declined thereafter. Although the reason for these discrepancies between studies is unknown, Drury et al [13] observed a gradual increase in urinary 8-OHdG over the first month of life and speculated that the reason may be as follows: as the growth velocity curve for neonates increases steadily until a peak is reached between 4 and 6 weeks of age, with a gradual decrease thereafter until about 6 months when the velocity is comparatively stable [24], and as neonates that are growing rapidly are likely to have a larger pool of free nucleotides, which are much more prone to oxidative damage than nucleotides incorporated into DNA, in addition to the high rate of cell division resulting in the removal of the nuclear membrane and histones, DNA may be exposed to a much higher concentration of oxygenderived free radicals and thus be more vulnerable to oxidative damage [13].…”

Section: Discussionmentioning

confidence: 75%

“…Although suggested by the results of two previous studies by Tsukahara et al [20] and Joung et al [15], it has not been demonstrated previously that the dose of supplemental oxygen is associated with urinary 8-OHdG level. In the study by Tsukahara et al [20] examining urinary 8-OHdG levels in two groups of preterm infants at the ages of 1 week and 1 month, 12 sick preterm neonates requiring supplemental oxygen indeed exhibited significantly higher urinary 8-OHdG levels (112±63 vs. 67±50 ng/mg Cr, respectively) at 1 month compared to 27 clinically stable preterm infants not requiring supplemental oxygen.…”

Section: Discussionmentioning

confidence: 82%

“…In the study by Tsukahara et al [20] examining urinary 8-OHdG levels in two groups of preterm infants at the ages of 1 week and 1 month, 12 sick preterm neonates requiring supplemental oxygen indeed exhibited significantly higher urinary 8-OHdG levels (112±63 vs. 67±50 ng/mg Cr, respectively) at 1 month compared to 27 clinically stable preterm infants not requiring supplemental oxygen. However, the 12 sick neonates, in comparison with the 27 control neonates, were born at significantly earlier stages of pregnancy (28.5±2.8 vs. 32.2±1.8 weeks, respectively) with significantly smaller birth weight (1094±378 vs. 1693±383 g, respectively) and lower 1-and 5-min Apgar scores (5±2 vs. 8±1 for 1-min and 7±2 vs. 9±1 for 5-min, respectively).…”

Section: Discussionmentioning

confidence: 93%

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Effects of supplemental oxygen on urinary 8-hydroxy-2’-deoxyguanosine levels in extremely low birth weight infants

Kato

Ibara

Kumazawa

et al. 2014

Free Radical Research

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As the effects of supplementary oxygen on urinary excretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG) are poorly understood, urinary 8-OHdG levels (ng/mg creatinine) were determined longitudinally on postnatal day (PND) 1, 3, and 30 in 16 neonates with birth weight <1000 g. No supplementary oxygen was required in 9 neonates during the first 24 h of life. Urinary 8-OHdG level on PND 1 was inversely correlated with birth weight in these 9 neonates (P=0.0323) and was higher in four with birth weight <750 g than five with birth weight >750 g (41.0±6.9 vs. 5.6±2.7, respectively, P=0.0200). Median urinary 8-OHdG on PND 1 of these 9 neonates was significantly lower than that of 7 neonates with oxygen (9.3 vs. 60.2, respectively), although there were no significant differences in clinical background, such as birth weight, between the two groups. Five of the 9 did not require supplemental oxygen at all during the first 30 days of life. Median urinary 8-OHdG levels were consistently significantly lower in the 5 neonates than in 11 neonates with oxygen transiently or persistently (9.3 vs. 54.6, 19

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“…It allows a noninvasive measurement of oxidative stress in vivo and enables repeated monitoring under disease conditions (20). It has been used for the measurement of oxidative stress in preterm neonates urine (21), and in a big population study aiming to clarify if oxidative damage to DNA may be important in carcinogenesis and a possible risk factor for lung cancer (22). The oxidation of guanine in DNA is not the only source of the urinary 8-oxodG.…”

Section: Discussionmentioning

confidence: 99%

Resuscitation of Hypoxic Newborn Piglets With Oxygen Induces a Dose-Dependent Increase in Markers of Oxidation

et al. 2007

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Newborn resuscitation with pure oxygen may be associated with long-term detrimental effects. Due to the change in attitude toward use of less oxygen upon resuscitation, there is a need to study effects of intermediate hyperoxia. The aim was to study dose-response correlation between inspiratory fraction of oxygen used for resuscitation and urinary markers of oxidative damage to DNA and amino acids. Hypoxemia was induced in newborn piglets following a standardized model; they were resuscitated for 15 min with either 21%, 40%, 60% or 100% oxygen and observed for 1 h. Urine samples were collected. Urinary elimination of 8-hydroxy-2=-deoxyguanosine (8-oxo-dG), 2=deoxyguanosine (2dG), orthotyrosine (o-Tyr) and phenylalanine (Phe) were determined by HPLC and tandem mass spectrometry (HPLC-MS/MS). Quotient of 8-oxo-dG/ 2dG and o-Tyr/Phe ratios were significantly and dose-dependant higher in piglets resuscitated with supplementary oxygen. T he transition from fetal to neonatal life contains complex and rapid physiologic changes. Usually, these changes are spontaneous, but approximately 10% of newborn babies require some assistance to begin breathing, and about 1% requires extensive resuscitation (1).Compared with the intrauterine pO 2 at about 3.3 kPa every child comes out to a relative hyperoxia and the values will normally spontaneously rise to about 8kPa during the first 30 min pp. Excessive hyperoxia may disturb a tuned equilibrium in the oxidative-antioxidative system. Exposure to hyperoxia generates significant higher levels of free radicals, and even more when preceded by hypoxia (2).In the neonatal literature an increasing number of studies focusing on supplementary oxygen shows how oxidative stress influences both mortality and morbidity (3,4). Moreover, babies exposed to high oxygen concentrations in the first minutes of postnatal life have been shown to be at higher risk of childhood leukemia and cancer (5,6) making it mandatory to search for the causal connection.We wanted to explore possible reasons for long-term effects of oxidative stress as well as to search for signs of oxidative damage to DNA and proteins and have chosen two biomarkers of oxidative stress; 8-hydroxy-2=-deoxyguanosine (8oxodG) and ortho-tyrosine (o-Tyr). Urinary excretion of modified nucleosides/bases i.e., 8oxodG can be measured to assess oxidative damage on the level of the whole organism (7) and has been used to evaluate oxidative stress in patients (8). O-tyr is known to be formed entirely upon reaction of Phe with hydroxyl radicals and can also be measured in urine (9).In addition, we also wanted to study the effect of re oxygenation with different oxygen concentrations to see if there is a dose-dependency or if there could be a threshold beyond which supplementary oxygen seems to be detrimental.We hypothesized that resuscitation of piglets with supplementary oxygen (40%, 60% or 100%) would cause increased oxidative stress, more reactive oxygen species (ROS), and more damage to proteins and DNA than 21% oxygen. MATERIALS ...

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Quantitation of glutathione S transferase‐π in the urine of preterm neonates

Abstract: These results indicate the potential effect of systemic oxidative stress on urinary excretion of GST-pi. Further studies are necessary to explore the effect of oxidative conditions on expression of GST-pi in distal tubules in the human kidney.

Cited by 13 publications

References 19 publications

Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

Effects of supplemental oxygen on urinary 8-hydroxy-2’-deoxyguanosine levels in extremely low birth weight infants

Resuscitation of Hypoxic Newborn Piglets With Oxygen Induces a Dose-Dependent Increase in Markers of Oxidation

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