Quantitation of hepatitis C virus RNA in saliva and serum of patients coinfected with HCV and human immunodeficiency virus

Rey, David; Fritsch, S.; Schmitt, Carine; Meyer, P.; Lang, J. M.; Stoll‐Keller, Françoise

doi:10.1002/1096-9071(20000201)63:2<117::aid-jmv1005>3.0.co;2-1

Cited by 27 publications

(10 citation statements)

References 14 publications

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“…To our knowledge, to date only Rey et al 21 have simultaneously studied the HCV viral load in serum and saliva of a group of HCV-HIV-coinfected patients without finding a significant relation between them. However, that study was performed by applying analytical quantification techniques designed to detect the virus in blood and not in other corporal fluids such as saliva, and their results should therefore be interpreted with caution.…”

Section: Discussionmentioning

confidence: 99%

Detection of HCV-RNA in Saliva of HIV-HCV Coinfected Patients

Eirea

Dios

Hermida

et al. 2005

AIDS Research and Human Retroviruses

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The presence of HCV-RNA in saliva of patients with chronic hepatitis C provides a biological basis for the potential transmission of this virus. HCV viremia is particularly high in HCV-HIV-coinfected patients, which could favor the presence of HCV in their saliva. This study was designed to evaluate the prevalence of HCV in saliva of HCV-HIV-coinfected patients. Stimulated whole saliva was collected from 75 HCV-HIV-coinfected patients and 75 HCV controls. The presence of HCV-RNA in saliva was tested by a highly sensitive noncommercialized nested PCR, and analyzed in relation to demographic, clinical, and analytical variables. HCVRNA was detected in the saliva of 49 (65%) HCV-HIV-coinfected patients and 39 (52%) HCV controls. The presence of HCV in saliva was not related to any of the analyzed variables in HCV-HIV-coinfected patients. In the HCV control group a statistically significant relationship was demonstrated only between the detection of HCV-RNA in saliva and the viral load in peripheral blood (p < 0.001). Our results indicate that there is a trend toward a higher HCV-RNA prevalence in the saliva of HCV-HIV-coinfected patients.

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Section: Discussionmentioning

confidence: 99%

Detection of HCV-RNA in Saliva of HIV-HCV Coinfected Patients

Eirea

Dios

Hermida

et al. 2005

AIDS Research and Human Retroviruses

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“…HIV-1 infection enhances HCV replication, thus changing the course of HCV-related disease in co-infected patients [6,7]. HCV was originally thought to be strictly hepatotropic, while the main cell targets for HIV-1 infection are mononuclear leukocytes bearing CD4 and the chemokine receptors C-C chemokine receptor type 5 (CCR5) and chemokine (C-X-C motif) receptor 4 (CXCR4).…”

Section: Introductionmentioning

confidence: 99%

The Tat protein of human immunodeficiency virus-1 enhances hepatitis C virus replication through interferon gamma-inducible protein-10

Zhang

et al. 2012

BMC Immunol

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BackgroundCo-infection with human immunodeficiency virus-1 (HIV-1) and hepatitis C virus (HCV) is associated with faster progression of liver disease and an increase in HCV persistence. However, the mechanism by which HIV-1 accelerates the progression of HCV liver disease remains unknown.ResultsHIV-1/HCV co-infection is associated with increased expression of interferon gamma-induced protein-10 (IP-10) mRNA in peripheral blood mononuclear cells (PBMCs). HCV RNA levels were higher in PBMCs of patients with HIV-1/HCV co-infection than in patients with HCV mono-infection. HIV-1 Tat and IP-10 activated HCV replication in a time-dependent manner, and HIV-1 Tat induced IP-10 production. In addition, the effect of HIV-1 Tat on HCV replication was blocked by anti-IP-10 monoclonal antibody, demonstrating that the effect of HIV-1 Tat on HCV replication depends on IP-10. Taken together, these results suggest that HIV-1 Tat protein activates HCV replication by upregulating IP-10 production.ConclusionsHIV-1/HCV co-infection is associated with increased expression of IP-10 mRNA and replication of HCV RNA. Furthermore, both HIV-1 Tat and IP-10 activate HCV replication. HIV-1 Tat activates HCV replication by upregulating IP-10 production. These results expand our understanding of HIV-1 in HCV replication and the mechanism involved in the regulation of HCV replication mediated by HIV-1 during co-infection.

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“…Although the presence of HCV-RNA in saliva has been reported by several research groups, only limited studies have attempted to identify HCV-RNA in saliva, in which patients coinfected with HCV and HIV were examined using a branched DNA assay [35]. Few studies have investigated the occurence of human viruses in GCF [13, 14, 36, 37].…”

Section: Discussionmentioning

confidence: 99%

Correlation of Hepatitis C Antibody Levels in Gingival Crevicular Fluid and Saliva of Hepatitis C Seropositive Hemodialysis Patients

Açıkgöz

Cengiz

Keskiner

et al. 2009

International Journal of Dentistry

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Search for hepatitis C virus (HCV) in body fluids other than blood is important when assessing possible nonparenteral routes of viral transmission. However, the role of oral fluids in HCV transmission remains controversial. Our aim was to compare the prevalence of HCV antibody (HCV Ab) levels in saliva, and gingival crevicular fluid (GCF) of HCV seropositive hemodialysis patients. Serum, saliva and GCF samples were collected from thirty-nine patients. Samples were analyzed for HCV Ab using the Ortho HCV 3.0 SAVe enzyme-linked immunosorbent assay (ELISA). HCH Ab levels in saliva and GCF of all HCV-seropositive patients were statistically compared. Reported here are the results of the study designed to determine the correlation between HCV-RNA positivity in serum and the detection of antibodies in GCF and saliva. One hundred percent (100%) of the 39 patients have antibodies to HCV in their serum, 15.4% have antibodies to HCV in GCF, and saliva found out. HCV Ab seropositivity in GCF and saliva was significantly correlated (kappa = 0.462; P < .001). This study supports the concept that GCF may be a significant source of HCV in saliva.

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Quantitation of hepatitis C virus RNA in saliva and serum of patients coinfected with HCV and human immunodeficiency virus

Cited by 27 publications

References 14 publications

Detection of HCV-RNA in Saliva of HIV-HCV Coinfected Patients

Detection of HCV-RNA in Saliva of HIV-HCV Coinfected Patients

The Tat protein of human immunodeficiency virus-1 enhances hepatitis C virus replication through interferon gamma-inducible protein-10

Correlation of Hepatitis C Antibody Levels in Gingival Crevicular Fluid and Saliva of Hepatitis C Seropositive Hemodialysis Patients

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