Quantitation of human herpesvirus 8 (HHV‐8) antibody in patients transfused with HHV‐8–seropositive blood

Fowlkes, Ashley; Brown, Cedric; Amin, Minal M.; Roback, John D.; Downing, Robert; Nzaro, E.; Mermin, Jonathan; Hladik, Wolfgang; Dollard, Sheila C.

doi:10.1111/j.1537-2995.2009.02269.x

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…A previous study on HHV8 transmission has shown that transmission of HHV8 via blood is inefficient [25]. In fact passive transfer of HHV8 antibody was even suggested to have a protective effect against HHV8 transmission [26]. In our study, no association between sexual behaviour, including multiple sex patterns and condom use, and HHV8‐positive status was observed.…”

Section: Discussioncontrasting

confidence: 60%

Prevalence of human herpesvirus 8 and hepatitis C virus in a rural community with a high risk for blood-borne infections in central China

Ding

Crabtree

et al. 2011

Clinical Microbiology and Infection

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Former illegal blood donation in the past decade has caused HIV outbreaks in some rural areas in China. Other HIV associated virus infections, such as human herpesvirus 8 (HHV8) in such areas are still not well defined. In order to explore HHV8 and HCV seroprevalence and potential risk factors in such areas, a cross-sectional study with 305 HIV positive and 315 HIV negative subjects recruited from a rural county in Shanxi province was conducted, where illegal blood collection was reported. Interview questionnaires and serum testing were carried out with these participants. HCV and HHV8 seroprevalence were found to be higher in HIV positive than negative group (76.4% vs. 2.5%; 15.4% vs. 4.8% respectively), while the difference in HBV seroprevalence was not significant. Co-infection with HCV and HHV8 was also more prevalent in the HIV positive group. HIV status (odds ratio [OR], 2.71; 95% confidence interval [CI], 1.16–6.30) and HBV status (OR, 2.56; 95%CI: 1.14–5.75) were independently associated with HHV8 infection. HIV status (OR, 23.03; 95%CI: 9.95–53.27) and blood/plasma selling history (OR, 14.57; 95%CI: 7.49–28.23) were strongly associated with HCV infection. These findings demonstrate that both HHV8 and HCV infections are prevalent in this community. HIV infection is an important risk factor for both HHV8 and HCV infection. HBV infection is associated with HHV8 infection but not with HCV infection. It is possible that HHV8 and HBV, but not HCV, may have similar mode of transmission in this population.

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Section: Discussioncontrasting

confidence: 60%

Prevalence of human herpesvirus 8 and hepatitis C virus in a rural community with a high risk for blood-borne infections in central China

Ding

Crabtree

et al. 2011

Clinical Microbiology and Infection

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“…Storing blood for several hours or days would also likely reduce the risk of transmission since we have observed that KSHV DNA detection is frequently reduced or abolished if blood from KSHV infected subjects is stored overnight before DNA extraction [Whitby and Uldrick, unpublished observations] These measures seem to be preferable to the introduction of screening blood or blood products for KSHV antibodies given the limitations of currently available assays[47] [48]. Such screening may even be counter-productive since a recent report demonstrated that transmission of KSHV occurred less frequently when donors had higher levels of antibodies[49]. The relationship between antibody titre, KSHV viral load and transmissibility of KSHV is clearly complex[50; 51]and is an area that warrants further study.…”

Section: Kshv Transmissionmentioning

confidence: 99%

Update on KSHV epidemiology, Kaposi Sarcoma pathogenesis, and treatment of Kaposi Sarcoma

Uldrick¹,

2011

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“…Recipient 2027, negative for IgG to HHV‐8 before transfusion, seroconverted after receiving an antibody and DNA positive whole blood transfusion. As demonstrated by Fowlkes and coworkers, 16 passive antibodies are detected immediately after transfusion with a relatively high titer and become undetectable in approximately 3 weeks. Immune response becomes detectable 4 to 10 weeks posttransfusion and is high titer.…”

Section: Discussionmentioning

confidence: 79%

Human herpesvirus 8 transfusion transmission in Ghana, an endemic region of West Africa

Gobbini

Owusu‐Ofori²,

Marcelin³

et al. 2012

Transfusion

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BACKGROUND: Human herpesvirus 8 (HHV‐8) seroprevalence ranges between less than 5% in Europe and North America and 50% to 70% in sub‐Saharan Africa. Evidence of HHV‐8 transfusion transmission is only indirect. We conducted a serologic (anti‐HHV‐8) and molecular (HHV‐8 DNA) study of samples from paired donor‐immunocompetent recipients transfused with whole blood. STUDY DESIGN AND METHODS: Samples from 252 donor‐recipient pairs were tested. Immunoglobulin G to HHV‐8 was detected with enzyme immunoassays and confirmed with an in‐house immunofluorescence assay. The cellular fraction from seroreactive donors and their recipients was tested for HHV‐8 DNA. RESULTS: Anti‐HHV‐8 was positive (reactive in two or more assays) in 28 (11%) patients and 16 (6%) donors. Of 12 seronegative recipients (at risk of transmission) receiving seropositive blood, one very likely transmission was identified (8.3% confidence interval, 0%‐23%). The donor blood contained HHV‐8 DNA and his and four other donors' sequences clustered separately from recorded genotypes with a 97% bootstrap constituting a distinct genotype. CONCLUSIONS: HHV‐8 is transmitted in Ghana but does not carry clinical consequences since most patients are immunocompetent. The clinical risk will increase with the availability of immunosuppressive drugs in sub‐Saharan Africa. We propose that a new genotype (HHV‐8‐G for Ghana) be added to the current nomenclature.

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Quantitation of human herpesvirus 8 (HHV‐8) antibody in patients transfused with HHV‐8–seropositive blood

Abstract: The source of transient antibody in patients who received transfusions was determined to be the transfused blood. Donors with higher HHV-8 antibody titers may have been less likely to have infectious virus in the blood.

Cited by 5 publications

References 21 publications

Prevalence of human herpesvirus 8 and hepatitis C virus in a rural community with a high risk for blood-borne infections in central China

Prevalence of human herpesvirus 8 and hepatitis C virus in a rural community with a high risk for blood-borne infections in central China

Update on KSHV epidemiology, Kaposi Sarcoma pathogenesis, and treatment of Kaposi Sarcoma

Human herpesvirus 8 transfusion transmission in Ghana, an endemic region of West Africa

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