Quantitation of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography

Eyre, David R.; Koob, Thomas J.; Ness, Kirk P. Van

doi:10.1016/0003-2697(84)90101-5

Cited by 636 publications

(326 citation statements)

References 23 publications

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“…Another 395 nm fluorescence, due to pyridinoline, is present in hard tissue collagens (achilles tendon, cartilage, bone, dentin), but not in soft tissue collagens (skin, cornea, tail tendon) [15,16]. This study shows the presence of both 430 nm blue fluorescence and the novel 360-370 nm fluorescence in CNBr peptides from pyridinoline-free rat tail tendon collagen.…”

Section: Discussionmentioning

confidence: 81%

Cross‐linking of collagen CNBr peptides by ozone or UV light

Fujimori

1988

FEBS Letters

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Insoluble collagen from rat tail tendon was digested with cyanogen bromide. The resultant peptides were dissolved in 0.1% SDS solution and separated by gel filtration and gel elcctrophoresis.Cross-linking occurred in CNBr-cleaved peptides when they were exposed to ozone or biologically effective UV (300 nm) radiation. The enhancement of a blue fluorescence at 430 nm (excited at 350 nm) was found to be associated with oxidized, cross-linked peptides. Polymeric peptides, formed in collagen with aging, also exhibited enhanced blue fluorescence.

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Section: Discussionmentioning

confidence: 81%

Cross‐linking of collagen CNBr peptides by ozone or UV light

Fujimori

1988

FEBS Letters

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“…IMC concentration was calculated, assuming that collagen weighed 7.25 times the measured hydroxyproline weight (Eastoe and Leach, 1958) and expressed as µg hydroxyproline/mg of lyophilized tissue. Hydroxylysylpyridinoline (HLP) concentration, the principal non-reducible crosslink of muscle collagen (McCormick, 1999), was determined using the high pressure liquid chromatography (HPLC) procedure developed by Eyre et al (1984). Hydroxylysylpyridinoline was expressed as moles of HLP per mole of collagen and also as µg HLP/mg of lyophilized tissue.…”

Section: Collagen Analysismentioning

confidence: 99%

Growth, Carcass and Meat Quality of Casertana, Italian Large White and Duroc x (Landrace x Italian Large White) Pigs Reared Outdoors

Maiorano

Gambacorta

Tavaniello

et al. 2013

Italian Journal of Animal Science

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To compare growth, skeletal development, carcass traits and meat quality of different genotypes, 10 Casertana (CT), 10 Italian Large White (LW) and 10 Duroc x (Landrace x Italian Large White) (DU) crosses, barrows of 90 days of age, were allotted to the same outdoor rearing and feeding conditions. Live weight was recorded and average daily gain (ADG) was calculated. At slaughter (330-day-old) dressing and lean percentages were determined; backfat thickness and loin eye depth were measured. Carcasses were dissected into commercial cuts. Water holding capacity, pH and colour (45 min and 24 h post-mortem) were measured. Longissimus lumborum muscle samples were collected for cholesterol, α-tochopherol and intramuscular collagen (IMC) analyses. CT compared to DU and LW had the lowest growth rate and skeletal development.Casertana showed higher backfat thickness, lower lean cut/fatty cut ratio and less lean meat (P<0.05). Loin eye depth differed among genotypes with LW>DU>CT (P<0.05). CT showed higher red colour of the meat than DU and LW (P<0.05). CT compared to LW had the highest hydroxylysylpiridinoline (HLP) crosslink concentration and HLP/IMC ratio, and a lower IMC amount (P<0.05). Casertana pigs produced meat that could be tougher than that from the improved breed, but more acceptable from the technological point of view.At eleven months of age bone weight, length and diameter were clearly genetic type-related; differently, the bone maturity was similar among the genotypes studied.

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“…Peaks were detected by fluorescence (20) and quantitated by external standards. Values are expressed as 24-h excretions (CV of 8 and 13% for PYD and DPD, respectively).…”

Section: Bone Turnovermentioning

confidence: 99%

Energy Restriction Reduces Fractional Calcium Absorption in Mature Obese and Lean Rats

Cifuentes

Morano

Chowdhury

et al. 2002

The Journal of Nutrition

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Weight loss is associated with bone loss and the risk may be greater in lean than heavier individuals, but the mechanisms involved remain unclear. We hypothesized that energy restriction (EnR) would decrease true fractional Ca absorption (TFCA) and be mediated by Ca-regulating hormones, but differently in obese and lean rats. Rats were fed a high fat (47% energy) or low fat (16% energy) diet for 4 mo. At 6 mo of age, the resulting lean [284 ± 28g (mean ± SD, n = 18)] and obese (319 ± 34g, n = 20) groups (P < 0.005) were divided into controls (CTL, ad libitum) and energy-restricted (40% restriction) groups. At baseline, bone resorption (urinary crosslinks) was higher and bone formation (serum osteocalcin) was lower in obese than in lean rats, whereas Ca balance components and Ca-regulating hormones did not differ. EnR for 10 wk reduced body weight by 25 ± 7% compared with a 6 ± 6% gain in CTL rats (P < 0.001). For both lean and obese rats, TFCA (5-d measurement, 45 Ca radioisotope) decreased from 30 ± 9% to 24 ± 9% with EnR, compared with 25 ± 10% to 29 ± 11% in controls (P < 0.05). Weight loss was directly correlated with the decrease in TFCA (r = 0.34, P < 0.05). Uterine weights indicated a reduced estrogenic activity in energy-restricted rats (P < 0.0001). In lean, but not obese rats, serum estradiol (E 2 ) correlated with weight loss (r = 0.52, P < 0.05), and tended to correlate with the decrease in TFCA (r = 0.48, P = 0.06). At the end of the study, serum 25-hydroxyvitamin-D was lower and urinary Ca was higher in lean than obese energy-restricted rats. Distinct endocrine profiles during weight loss in obese and lean rats suggest that the susceptibility of bone and Ca metabolism to EnR could differ depending on initial body weight.

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Quantitation of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography

Cited by 636 publications

References 23 publications

Cross‐linking of collagen CNBr peptides by ozone or UV light

Cross‐linking of collagen CNBr peptides by ozone or UV light

Growth, Carcass and Meat Quality of Casertana, Italian Large White and Duroc x (Landrace x Italian Large White) Pigs Reared Outdoors

Energy Restriction Reduces Fractional Calcium Absorption in Mature Obese and Lean Rats

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