1982
DOI: 10.3109/10641968209062382
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Quantitation of Inactive Renin in Human and Dog Plasma: Techniques for Activation

Abstract: For human samples quantitation of inactive renin can be carried out by incubation with trypsin under defined conditions, followed by RIA of the activated renin. For dog samples we were unable to obtain evidence for the presence of inactive renin in the plasma by using trypsin, acid or cold to activate. Increases in angiotensin generation did occur with trypsin and acid but they both changed renin substrate such that the rate of angiotensin generation by exogenous renin was increased at pH 7.4, but not at pH 5.… Show more

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Cited by 3 publications
(3 citation statements)
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“…They were compared with matched patients with primary (essential) hypertension and to healthy normotensive individuals. We measured plasma renin activity (PRA) [14], plasma aldosterone concentration (PAC) [15], and active and total trypsin-activated renin [16,17], and calculated the plasma levels of prorenin as difference between the latter and the former.…”
Section: Plasma Levels Of Prorenin In Patients With Primary Aldosteromentioning
confidence: 99%
“…They were compared with matched patients with primary (essential) hypertension and to healthy normotensive individuals. We measured plasma renin activity (PRA) [14], plasma aldosterone concentration (PAC) [15], and active and total trypsin-activated renin [16,17], and calculated the plasma levels of prorenin as difference between the latter and the former.…”
Section: Plasma Levels Of Prorenin In Patients With Primary Aldosteromentioning
confidence: 99%
“…The serum concentration of vWF was measured by microenzyme-linked immunoadsorbent assay, as described previously [29]. Plasma concentration of prorenin was determined by RIA as described previously [30]. Peripheral blood was drawn between 09.00 and 09.30 hours, after at least 30 min rest in the supine position.…”
Section: Methodsmentioning
confidence: 99%
“…9 All these activations are time consuming and have their technical limitations. 10 " 17 For these reasons it is desirable to develop sensitive and rapid radioimmunoassays for active renin and prorenin. In the past few years, efforts have been made toward developing monoclonal antibodies that are selective for prorenin and active renin.…”
mentioning
confidence: 99%