Quantitation of intestinal villus cells on a weight basis: values for C57BL-6 mice

Rf, Hagemann; Cp, Sigdestad; Lesher, S.

doi:10.1152/ajplegacy.1970.218.3.637

Cited by 14 publications

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“…Hence it appears that the drug results in partial syn chronization of these cells populations in vivo. In light of this finding, it is not surprising that the response of crypt and tumor cells to AME is simi lar since these cells have similar generation times; 13 h [4] and 14 h [1] respectively.…”

Section: Discussionmentioning

confidence: 92%

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Biological Effects of Anthramycin Methyl Ether

Sigdestad¹,

Hagemann²,

Lesher³

1970

Chemotherapy

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The temporal course of DNA synthesis after subcutaneous injection on Anthramycin Methyl Ether (AME) was investigated in Sarcoma-180 ascites tumor cells and in the proliferative cells of the intestinal epithelium. Both cell types demonstrated dramatic inhibition of DNA synthesis: however, there was no appreciable difference in response of the 2 cell systems to the drug. After sub-lethal doses, recovery was seen to occur in an oscillatory fashion which eventually reached control values. This pattern of recovery suggested partial in vivo synchronization of the proliferative cell population, which could be due to preferential block in one stage of cell cycle or selective killing of cells in a sensitive stage.

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Section: Discussionmentioning

confidence: 92%

“…The assay of DNA synthesis in S-180 tumor cells utilized 5 or 10 «Ci 3HTdR, 6.0 Ci/mmole, depending upon whether the assay in vitro or in vivo. 4. Intestinal tritium assay.…”

Section: Methodsmentioning

confidence: 99%

Biological Effects of Anthramycin Methyl Ether

Sigdestad¹,

Hagemann²,

Lesher³

1970

Chemotherapy

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“…The fixed tissues were rinsed in 60% ethanol, solubilized in Soluene (Schwartz/Mann, Orangeburg, N.Y.) and counted in a liquid scintillation spectrometer. The incorporation of [3H]TdR (dpm/mg wet weight) was determined as an estimate of proliferative activity of the tissues (Hagemann, Sigdestad & Lesher, 1970a, 1970b. Previously, we have established that ['HjTdR dpm/mg wet weight in the intestinal mucosa in TB mice accurately reflects the proliferative activity by comparing this value with both the number of labelled cells per crypt and the ['HITdR incorporation into individual crypt cells (Kovacs et af., 1981).…”

Section: Gastrointestinal Proliferationmentioning

confidence: 99%

Tumour‐induced altered gastrointestinal steady‐states: absence of MHC restriction in the paraneoplastic gastrointestine

et al. 1989

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The growth of a number of experimental rodent tumours including the Lewis lung tumour (LLca) progressively compromises the integrity of the host's gastrointestine by inducing cytokinetic alterations in the small bowel resembling those generally defining the intestinal phase of a graft‐versus‐host reaction (GVHR). To determine whether the induction of this paraneoplastic gastrointestine (PGI) involves, similar to a GVHR, a disparity between the MHC of the donor (LLca tumour) and the recipient (host), PGI development was evaluated in various LLca tumour‐bearing murine strains that were either ‘syngeneic’[C57BL/6 and BL/10 (H‐2b)], ‘semisyngeneic’[B6D2F1 (H‐2bd) and B6C3F1 (H‐2bk)] or ‘allogeneic’[C3H/HeJ (H‐2k) and DBA/2 (H‐2d)] to the H‐2b LLca tumour. The temporal appearance and magnitude of a PGI developing in either LLca‐syngeneic or semi‐syngeneic hosts, but not the allogeneic strains, suggested that the mechanism(s) involved in PGI development, like the GVHR, was restricted by the MHC. Subsequent studies using congenic strains [B10.A (H‐2k) and B10.D2/nSn (H‐2d)], however, demonstrated that the mechanism(s) responsible for the PGI was restricted by the non‐MHC loci of the C57BL mouse. These observations were supported by the appearance of a LLca‐induced PGI in various B10.A congenic strains carrying mutations at the I‐A or I‐E/I‐J loci of the MHC. Not unlike the intestinal phase of a GVHR, development of the PGI required the participation of enhanced mucosal mast cells which were limited in the WCB6F1 (S1/S1d) but not the (+/+) murine strains. These observations are discussed in light of the postulated premature migration of immature thymocytes that accompany tumour growth and their ability to non‐specifically enhance (or suppress) cell mediated immune reactions in the host.

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Direct quantitative estimation of Paneth and total cell populations in the jejunal glands of Lieberkühn

Malinin

1975

Am. J. Anat.

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Quantitation of intestinal villus cells on a weight basis: values for C57BL-6 mice

Cited by 14 publications

References 0 publications

Biological Effects of Anthramycin Methyl Ether

Biological Effects of Anthramycin Methyl Ether

Tumour‐induced altered gastrointestinal steady‐states: absence of MHC restriction in the paraneoplastic gastrointestine

Direct quantitative estimation of Paneth and total cell populations in the jejunal glands of Lieberkühn

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