Quantitation of levamisole in plasma using high performance liquid chromatography

Vandamme, Thierry F.; Demoustier, M.; Rollmann, Bruno

doi:10.1007/bf03226369

Cited by 32 publications

(18 citation statements)

References 7 publications

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“…The presence of levamisole is difficult to confirm as a result of its short half‐life (5.6 h), the lack of widespread access to testing, and the low sensitivity of urine and blood assays 10 . Management is supportive.…”

Section: Discussionmentioning

confidence: 99%

Clinical, histopathologic, and immunofluorescence findings in levamisole/cocaine‐induced thrombotic vasculitis

2013

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Section: Discussionmentioning

confidence: 99%

Clinical, histopathologic, and immunofluorescence findings in levamisole/cocaine‐induced thrombotic vasculitis

2013

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“…Levamisole concentrations were determined using a high-performance liquid chromatography method adapted from Vandamme et al [26]. …”

Section: Methodsmentioning

confidence: 99%

Randomized Controlled Trial of Levamisole Hydrochloride as Adjunctive Therapy in Severe Falciparum Malaria With High Parasitemia

Maude

Silamut

Plewes

et al. 2013

The Journal of Infectious Diseases

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Background. Cytoadherence and sequestration of erythrocytes containing mature stages of Plasmodium falciparum are central to the pathogenesis of severe malaria. The oral anthelminthic drug levamisole inhibits cytoadherence in vitro and reduces sequestration of late-stage parasites in uncomplicated falciparum malaria treated with quinine.Methods. Fifty-six adult patients with severe malaria and high parasitemia admitted to a referral hospital in Bangladesh were randomized to receive a single dose of levamisole hydrochloride (150 mg) or no adjuvant to antimalarial treatment with intravenous artesunate.Results. Circulating late-stage parasites measured as the median area under the parasite clearance curves were 2150 (interquartile range [IQR], 0–28 025) parasites/µL × hour in patients treated with levamisole and 5489 (IQR, 192–25 848) parasites/µL × hour in controls (P = .25). The “sequestration ratios” at 6 and 12 hours for all parasite stages and changes in microvascular blood flow did not differ between treatment groups (all P > .40). The median time to normalization of plasma lactate (<2 mmol/L) was 24 (IQR, 12–30) hours with levamisole vs 28 (IQR, 12–36) hours without levamisole (P = .15).Conclusions. There was no benefit of a single-dose of levamisole hydrochloride as adjuvant to intravenous artesunate in the treatment of adults with severe falciparum malaria. Rapid parasite killing by intravenous artesunate might obscure the effects of levamisole.

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“…Various techniques have been used for the determination of levamisole in biological fluids. They include gas chromatography with nitrogenphosphorus detection (GC-NPD) 2 and flame ionization (GC-FID) [3] and liquid chromatography (LC) with ultraviolet (UV) detection [4][5][6][7] . Several methods have been developed for levamisole quantification such as polarography 8 , by a turbidimetric method and flow-injection analysis 9 .…”

Section: Levamisolementioning

confidence: 99%

Potentiometric Membrane Sensors for Levamisole Determination

et al. 2016

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The ion pair (IP) of levamisole with BiI4 - (SbI4 - ) for the levamisole-selective sensor with a PVC membrane containing - ions were developed. Thermal behavior of obtained IP was investigated by differential thermal analysis that would show the thermal stability and the character of the decomposition of the complex. The thermolysis of Lev+BiI4 - IP undergoes three stages that fit a theoretical interpretation. The linearity ranges of levamisole sensors function are 7.9 ×10-6 – 1×10-1 (7.9 ×10-5 – 1×10-1 ) M. The Nernstian slope of 50.6 – 53.4 mV pC−1 and detection limit of 5.0 × 10−5 – 1.5 × 10−4) M. The working range of pH is 2.8 – 6.0. The efficiency of the use of electrodes for levamisole content control in pharmaceutical preparations was shown by direct potentiometry and potentiometric titration methods.

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Quantitation of levamisole in plasma using high performance liquid chromatography

Cited by 32 publications

References 7 publications

Clinical, histopathologic, and immunofluorescence findings in levamisole/cocaine‐induced thrombotic vasculitis

Clinical, histopathologic, and immunofluorescence findings in levamisole/cocaine‐induced thrombotic vasculitis

Randomized Controlled Trial of Levamisole Hydrochloride as Adjunctive Therapy in Severe Falciparum Malaria With High Parasitemia

Potentiometric Membrane Sensors for Levamisole Determination

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