Quantitation of metolazone in plasma and urine by high-performance liquid chromatography with fluorescence detection

Vose, CW; Muirhead, DC; Evans, Gary; Stevens, P. M.; Burford, S.R.

doi:10.1016/s0378-4347(00)81067-1

Cited by 12 publications

(4 citation statements)

References 7 publications

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“…Also, few methods have been reported for the quantitative determination of MET in biological samples. These methods include HPLC for the determination of MET either alone or with furosemide . Also, liquid chromatography‐tandem mass spectrometry (LC‐MS) has been reported for the analysis of MET in human pasma and blood.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

et al. 2013

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A new, specific and sensitive reversed-phase high-performance liquid chromatography method was developed for the simultaneous determination of metolazone (MET) and losartan potassium (LOS). Good chromatographic separation was achieved within 6.0 min on a 150 × 4.6 mm i.d., 5 µm Waters, Ireland and ProDIGY 5 ODS 3 100 A column. A mobile phase containing a mixture of methanol and 0.02 M phosphate buffer (65:35, v/v) at pH 3.0 was used. The analysis was performed at a flow rate of 1 mL/min with fluorescence detection at 410 nm after excitation at 230 nm. Aspirin (ASP) was used as an internal standard. The proposed method was rectilinear over 2.0-40.0 (MET) and 40.0-800.0 ng/mL (LOS), with limits of detection of 0.22 and 4.52 ng/mL and limits of quantification of 0.68 and 13.70 ng/mL for MET and LOS, respectively. The method was successfully applied for the simultaneous analysis of the studied drugs in their laboratory-prepared mixtures, single tablets and co-formulated tablets. Moreover, the method was applied to an in vitro drug release (dissolution) test. The method was further extended to the determination of LOS in spiked human plasma. Statistical evaluation and comparison of data obtained using the proposed and comparison methods revealed no significant difference between the two methods in addition to good accuracy and precision for the proposed method.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

et al. 2013

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“…With the deepening of research, in order to further understand the pharmacokinetic characteristics, it needs a method for the determination of MET and VAL in biological samples. According to the already available literatures, several methods have been described for the determination of MET either solely or in combination with other drugs in biological fluids using HPLC-UV, 8 HPLC-FLR, 9 and liquid chromatography-tandem mass spectrometry (LC-MS/MS). 10,11 Similarly, different methods has been published for the determination of VAL either single-handedly or together with other drugs in biological fluids including HPLC-UV, 12,13 HPLC-DAD, 14 HPLC-FLR, 15 and LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and valsartan in plasma by on-line SPE coupled with liquid chromatography/tandem mass spectrometry

Zhou¹,

Chen

Liu³

et al. 2017

Eur J Mass Spectrom (Chichester)

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Combination of metolazone (0.5 mg) and valsartan (80 mg) has been verified as a promising therapy treatment for hypertension. In order to facilitate to pharmacokinetic research, it needs a method for the simultaneously determination of metolazone and valsartan in biological samples. However, there are no relative reports so far. In order to facilitate to pharmacokinetic research, an on-line solid phase extraction coupled with liquid chromatography-tandem mass spectrometry method for the simultaneous determination of metolazone and valsartan in beagle dog plasma was developed and validated in this study. An on-line solid phase extraction column Retain PEP Javelin (10 mm × 2.1 mm) was used to remove impurities in plasma samples. The metolazone, valsartan and internal standard (losartan) were separated on a Poroshell 120 SB-C18 column (4.6 mm × 50 mm × 2.7 µm) with a gradient elution procedure. Acidified acetonitrile/water mixture was used as a mobile phase. The selected multiple-reaction monitoring mode in positive ion was performed and the parent to the product transitions m/z 366/259, m/z 436.2/291 and m/z 423.4/207 were used to measure the metolazone, valsartan and losartan. The method was linear over the range of 0.1-100 ng/mL and 1-1000 ng/mL for metolazone and valsartan, respectively. This method was validated in terms of specificity, linearity, sensitivity, precision, accuracy, matrix effect, and stability and then successfully applied to pharmacokinetic studies of the metolazone and valsartan combination tablets in beagle dogs.

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“…12,15 On the other hand, few methods have been reported for the determination of metolazone in biological samples. These methods include: HPLC for the determination of metolazone either alone, [16][17][18][19] or with furosemide. 20 Also, liquid chromatography-tandem mass spectrometry (LC-MS), [21][22][23][24] has been reported for the analysis of metolazone in human plasma or blood.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of metolazone and spironolactone in raw materials, combined tablets and human urine by high performance liquid chromatography

et al. 2013

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A new, specific and sensitive reversed-phase high performance liquid chromatographic method was developed for the simultaneous determination of two diuretic drugs; metolazone (MET) and spironolactone (SPL). Good chromatographic separation was achieved within 5.0 min on 150 mm Â 4.6 mm i.d., 5 mm particle size Spherisorb-ODS 2 C18 column. A mobile phase containing a mixture of methanol and 0.02 M phosphate buffer (70 : 30) v/v at pH 3.0 was used. The analysis was performed at a flow rate of 1 mL min À1 with UV detection at 235 nm. Xipamide (XPM) was used as an internal standard (IS). The proposed method was rectilinear over the ranges of 0.05-1.0 mg mL À1 and 0.5-10.0 mg mL À1 with limits of detection (LOD) of 0.009, 0.04 ng mL À1 and limits of quantification (LOQ) of 0.03, 0.11 mg mL À1 for MET and SPL, respectively. The suggested method was successfully applied for the simultaneous analysis of the studied drugs in their laboratory prepared mixtures, single tablets and coformulated tablets. The method was further extended to the determination of both drugs in spiked human urine. The mean percentage recoveries of MET and SPL in spiked human urine were 99.33 AE 2.37 and 99.72 AE 3.27, respectively. The proposed method was also applied for the determination of the studied drugs in the presence of some co-administered or co-formulated drugs without any interference.Statistical evaluation and comparison of the data obtained by the proposed and comparison methods revealed no significant difference between the two methods regarding accuracy and precision. Fig. 1 The structural formulae of the studied drugs, (a) metolazone, (b) spironolactone.

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Quantitation of metolazone in plasma and urine by high-performance liquid chromatography with fluorescence detection

Cited by 12 publications

References 7 publications

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

Simultaneous determination of metolazone and losartan potassium in their binary mixtures using high‐performance liquid chromatography with fluorimetric detection: application to combined tablets and spiked human plasma

Simultaneous determination of metolazone and valsartan in plasma by on-line SPE coupled with liquid chromatography/tandem mass spectrometry

Simultaneous determination of metolazone and spironolactone in raw materials, combined tablets and human urine by high performance liquid chromatography

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