Quantitation of Monosodium Glutamate Using Immobilized Glutamate Oxidase/Peroxidase and Flow Injection Analysis

Mankasingh, Utra; Narinesingh, Dyer; Ngo, T. T.

doi:10.1080/00032710008543198

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…It provides a flavoring function when added to food as the naturally occurring free glutamate, and is different from the four classic tastes of sweet, sour, salty and bitter. The extensive demand for MSG as a direct food enhancer has prompted rules for its use in various countries [25].…”

Section: Introductionmentioning

confidence: 99%

An advanced spectroscopic approach (LIBS in combination with AAS): a steadfast means to probe the elemental composition of monosodium glutamate

Rehan¹,

Rehan²,

Khan³

et al. 2019

Laser Phys.

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The elemental composition of monosodium glutamate (MSG) was probed by engaging a laser-induced breakdown spectroscopy (LIBS) analytical technique. The LIBS spectra of MSG were acquired in air pressure with an appropriate detector (LIBS2000 + , Ocean Optics) with an optical resolution of ~0.06 nm in the spectral range from 220 to 720 nm. Analyses of the acquired LIBS spectra revealed the presence of Ag, Ca, Mg, S and Na in the sample. To maximize the signal to noise ratio in the resulting LIBS spectra, the experimental parameters, such as pulse energy and gate delay, were optimized. The accomplishment of local thermodynamic equilibrium supposition was confirmed by the McWhirter's criterion and in view of the relaxation time, as well as the diffusion length of the atoms in the plasma. For the determination of relative concentrations of detected elements in MSG, we used three LIBSbased approaches, i.e. by setting the concentration of Na as the calibration, by setting the sum of all concentrations as the calibration and by the calibration curve method. The same sample was also subjected to the more standard analytical tool of atomic absorption spectroscopy, and its results were cross-compared with the findings of LIBS to confirm its validity. It was highly encouraging to find that both results were in sound agreement.

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Section: Introductionmentioning

confidence: 99%

An advanced spectroscopic approach (LIBS in combination with AAS): a steadfast means to probe the elemental composition of monosodium glutamate

Rehan¹,

Rehan²,

Khan³

et al. 2019

Laser Phys.

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“…H 2 O 2 has been detected by amperometric methods [16][17] or chromogenic reaction by using flow injection system [18] or test strip [19] .…”

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confidence: 99%

Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase

Alnokkari¹,

Ataie²,

Alasaf³

2013

Chinese Journal of Appplied Environmental Biology

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A rapid, sensitive and simple chromogenic method was developed for quantitative determination of monosodium glutamate (MSG) in food samples. The method incorporated a glutamate oxidase and peroxidase. The librated H 2 O 2 from a glutamate sample as a result of enzymatic action was measured using 4-aminoantipyrine and phenol as a chromogenic reagent at 502 nm. Glutamate calibration curve was linear up to 125 mmol L -1 with a detection limit of 2 mmol L -1 . The results of analyzing L-glutamate in various food samples using the approach were compared to those of a standard procedure employing HPLC method. MSG concentration in different samples ranged from 0.93 to 4.9 g/kg. This method is relatively sensitive and specific, without the need of pre-treatment for sample. Fig 5, Tab 1, Ref 25

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“…Spectrophotometers are economical, easy to handle, and the reagents used in the assay are not expensive. Some of the common co-substrates employed in colorimetric method include guaiacol [13], 2,2-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) [14], ophenylenediamine [15], tetramethylbenzidine, diaminobenzidine, and p-phenylenediamine [16], pyrogallol [17], 4-aminoantipyrine-phenol [18], catechol [19], 4-aminoantipyrine-aniline [20], 3-methyl-2-benzothiazolinonehydrazone hydrochloride(MBTH)-dimethylaminobenzoic acid [21], 4-amino-5-(p-aminophenyl)-1-methyl-2-phenylpyrazol-3-one with N-ethyl-N-sulfopropyl aniline sodium salt [22], p-phenylenediamine-dimethylaminobenzoic acid [23], and MBTH-10,11-dihydro-5H-benz[b, f]azepine [24]. However, these reagents have some limitations such as carcinogenicity of o-dianisidine and benzidine, and broader linearity range of pyrogallol and guaiacol due to their auto-oxidation.…”

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confidence: 99%

Kinetic Study of Peroxidase‐Catalyzed Coupling of Benzene‐1,4‐diamine and N‐(2‐Aminoethyl)naphthalen‐1‐amine: Development of Micromolar Hydrogen Peroxide Reaction System

Shivakumar

Nagaraja

Aradhana

et al. 2010

Helvetica Chimica Acta

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A novel chromogenic method to measure the peroxidase activity using para-phenylenediamine dihydrochloride (¼ benzene-1,4-diamine hydrochloride; PPDD) and N-(1-naphthyl)ethylenediamine dihydrochloride (¼ N-(2-aminoethyl)naphthalen-1-amine; NEDA) is presented. The PPDD entraps the free radical and gets oxidized to electrophilic diimine, which couples with NEDA to give an intense redcolored chromogenic species with maximum absorbance at 490 nm. This assay was adopted for the quantification of H 2 O 2 between 20 and 160 mm. Catalytic efficiency and catalytic power of the commercial peroxidase were found to be 4.47 Â 10 4 m À1 min À1 and 3.38 Â 10 À4 min À1, respectively. The catalytic constant (k cat ) and specificity constant (k cat /K m ) at saturated concentration of the co-substrates were 0.0245 Â 10 3 min À1 and 0.0445 mm À1 min À1, respectively. The chromogenic coupling reaction has a minimum interference from the reducing substances such as ascorbic acid, l-cystein, citric acid, and oxalic acid. The method being simple, rapid, precise, and sensitive, its applicability has been tested in the crude vegetable extracts that showed peroxidase activity.

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Quantitation of Monosodium Glutamate Using Immobilized Glutamate Oxidase/Peroxidase and Flow Injection Analysis

Cited by 9 publications

References 26 publications

An advanced spectroscopic approach (LIBS in combination with AAS): a steadfast means to probe the elemental composition of monosodium glutamate

An advanced spectroscopic approach (LIBS in combination with AAS): a steadfast means to probe the elemental composition of monosodium glutamate

Colorimetric Determination of Monosodium Glutamate in Food Samples Using Colorimetric Determination of Monosodium Glutamate in Food Samples Using L-glutamate Oxidase-glutamate Oxidase

Kinetic Study of Peroxidase‐Catalyzed Coupling of Benzene‐1,4‐diamine and N‐(2‐Aminoethyl)naphthalen‐1‐amine: Development of Micromolar Hydrogen Peroxide Reaction System

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