Quantitation of nifedipine in human plasma by on-line solid-phase extraction and high-performance liquid chromatography

Yritia, Mercedes; Parra, Pilar; Iglesias, Estupiñan; Barbanoj, J.M

doi:10.1016/s0021-9673(99)01094-8

Cited by 32 publications

(20 citation statements)

References 22 publications

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“…Recently, Weimann et al [27] adopted the mode of the low-temperature reversed-phase liquid chromatography, which performed at 1 • C on a single ODS column for separation of six types of 8-hydroxylated guanine modifications in urine without any sample pre-treatment. Actually, coupled-column HPLC is a very adaptable strategy for the direct analysis of target compounds in biological fluids, as indicated by studies [28,29], including also the analysis of purine nucleic acid components [30,31]. In this study, we described a fully automated method for the determination of 14 urinary nucleosides by coupled-column liquid chromatography with UV detection.…”

Section: Introductionmentioning

confidence: 99%

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Zheng

Yang

et al. 2005

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Zheng

Yang

et al. 2005

Journal of Chromatography B

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“…Several analytical methods have been described for the assay of nifedipine in pharmaceutical formulations and biological fluids. These methods include spectrophotometry [3,4,5,6], gas chromatography [5,6,7,8,9,10,11,12,13,14], liquid chromatography [6,15,16], high-performance thin-layer chromatography [17], high-performance liquid chromatography [18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41], flow-injection tandem mass spectrometry [42] polarography [43,44], linear-sweep voltammetry [45] and differential-pulse stripping voltammetry [46,47].…”

Section: Introductionmentioning

confidence: 99%

Cathodic adsorptive stripping square-wave voltammetric determination of nifedipine drug in bulk, pharmaceutical formulation and human serum

2003

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Nifedipine is a calcium-channel antagonist drug used in the management of angina pectoris and hypertension through inhibition of calcium influx. A fully validated sensitive cathodic adsorptive stripping square-wave voltammetry procedure was optimized for the determination of the drug at trace levels. The procedure was based on the reduction of the nitrophenyl group after the interfacial accumulation of the drug onto a hanging mercury drop electrode in Britton-Robinson buffer of pH 11.0. The optimal conditions of the procedure were found to be: accumulation potential=-0.9 V vs. Ag/AgCl/KCl(s)), accumulation time=30 s, scan increment=10 mV, pulse amplitude=50 mV and frequency=120 Hz. Under these conditions, a well-defined peak was obtained; its peak current showed a linear dependence on drug concentration in the range of 2x10(-9)-2x10(-7) mol L(-1) bulk nifedipine. The mean recoveries based on eight replicate measurements for 1x10(-8) and 5x10(-8) mol L(-1) bulk nifedipine solutions were 98.46+/-0.86% and 98.23+/-0.92%, respectively. A detection limit of 3.42x10(-10) mol L(-1) bulk nifedipine was achieved. The procedure was successfully applied for assay of the drug in tablets and spiked human serum with mean recoveries of 101.95+/-1.42% and 98.70+/-0.63%, respectively. The limit of detection of the drug in spiked human serum was found to be 3.90x10(-10) mol L(-1).

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“…Previous methods included solid-phase extraction (SPE), protein precipitation and Liquid-liquid extraction (LLE). On-line SPE is expensive and complex, especially for a large number of samples [ 13 ] . After protein precipitation, the samples remained impure and were with the risk of blocking and poor response [ 14 ] .…”

Section: A More Rapid Sensitive and Specifi C Hplc-ms/ms Methods Formentioning

confidence: 99%

A More Rapid, Sensitive, and Specific HPLC-MS/MS Method for Nifedipine Analysis in Human Plasma and Application to a Pharmacokinetic Study

Chen

Huang

et al. 2013

Drug Res

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A more rapid, sensitive and specific high-performance liquid chromatography coupled to ?tandem mass spectrometry (HPLC-MS/MS) was developed and validated for the quantification of nifedipine in human plasma, and applied to the pharmacokinetic study of nifedipine in Chinese healthy volunteers. Nifedipine and internal standard (IS) acetaminophen in plasma were extracted with ethyl acetate, separated on a C18 (150?mm?4.6?mm, 5??m) reversed-phase column, eluted with acetonitrile mixed with 5?mM ammonium acetate solution (pH=6.62) (60:40, v/v), ionized by negative ion pneumatically assisted electrospray and detected in the multi-reaction monitoring mode using precursor?product ions of m/z 354.1?222.2 for nifedipine and 150.1?107.1 for the IS. A single oral dose of 20?mg nifedipine sustained release tablets and blood samples (4?mL) was collected before and 1, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 8, 12, 24, and 36?h after administration. The main pharmacokinetic parameters of nifedipine, as Tmax, t1/2?, t1/2?, t1/2z, Cmax, AUC0~36, AUC0~? were 2.80?0.50?h, 6.78?2.52?h, 6.82?2.53?h, 6.69?2.22?h, 76.69?19.51 (ng/mL), 546.49?162.28 (ng???h/mL) and 564.05?176.74 (ng???h/mL), respectively. The calibration curve was linear over the concentration range of 0.17?102?ng/mL (r2>0.99, n=5) with a lower limit of quantification (LLOQ) of 0.17?ng/mL. The intra- and inter-day precision was less than 15% for all quality control samples at concentrations of 0.42, 6.53 and 81.60?ng/mL and the accuracy (relative error, RE) was ???3.92% to 7.31% at 3 quality control levels. The specificity, matrix effect, recovery, sensitivity, linearity, accuracy, precision and stabilities were validated, and can fulfill the requirement of pharmacokinetic study of nifedipine sustained release tablets in Chinese volunteers.

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Quantitation of nifedipine in human plasma by on-line solid-phase extraction and high-performance liquid chromatography

Cited by 32 publications

References 22 publications

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Cathodic adsorptive stripping square-wave voltammetric determination of nifedipine drug in bulk, pharmaceutical formulation and human serum

A More Rapid, Sensitive, and Specific HPLC-MS/MS Method for Nifedipine Analysis in Human Plasma and Application to a Pharmacokinetic Study

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