Quantitation of nucleoprotein complexes by UV absorbance and Bradford assay

Jiang, Chen; Luo, Hao; Mei, Tao; Liu, Zhongchuan; Wang, Ganggang

doi:10.52601/bpr.2021.210028

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Western blotting analysis was utilized to determine CRP protein expression. Antibodies for Western blotting were a goat anti-mouse CRP polyclonal antibody (1:4000, Cat#: BAF1829, R&D Systems, Inc, Minneapolis, MN, USA), a rabbit anti-mouse β-actin polyclonal antibody (1:10000, Cat#: bs-0061R, Bioss Technology, Beijing, China), and an horseradish peroxidase-conjugated donkey anti-goat polyclonal antibody (1:5000, Cat#: EK030, Zhuangzhi Biological Technology, Xi’an, China) or goat anti-rabbit polyclonal antibody (1:10000, Cat#: bs-40295G, Bioss Technology) ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms

Fu,

Liu,

et al. 2023

Front. Immunol.

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BackgroundC-reactive protein (CRP) levels are elevated in patients with abdominal aortic aneurysms (AAA). However, it has not been investigated whether CRP contributes to AAA pathogenesis.MethodsCRP deficient and wild type (WT) male mice were subjected to AAA induction via transient intra-aortic infusion of porcine pancreatic elastase. AAAs were monitored by in situ measurements of maximal infrarenal aortic external diameters immediately prior to and 14 days following elastase infusion. Key AAA pathologies were assessed by histochemical and immunohistochemical staining procedures. The influence of CRP deficiency on macrophage activation was evaluated in peritoneal macrophages in vitro.ResultsCRP protein levels were higher in aneurysmal than that in non-aneurysmal aortas. Aneurysmal aortic dilation was markedly suppressed in CRP deficient (aortic diameter: 1.08 ± 0.11 mm) as compared to WT (1.21 ± 0.08 mm) mice on day 14 after elastase infusion. More medial elastin was retained in CRP deficient than in WT elastase-infused mice. Macrophage accumulation was significantly less in aneurysmal aorta from CRP deficient than that from WT mice. Matrix metalloproteinase 2 expression was also attenuated in CRP deficient as compared to WT aneurysmal aortas. CRP deficiency had no recognizable influence on medial smooth muscle loss, lymphocyte accumulation, aneurysmal angiogenesis, and matrix metalloproteinase 9 expression. In in vitro assays, mRNA levels for tumor necrosis factor α and cyclooxygenase 2 were reduced in lipopolysaccharide activated peritoneal macrophages from CRP deficient as compared to wild type mice.ConclusionCRP deficiency suppressed experimental AAAs by attenuating aneurysmal elastin destruction, macrophage accumulation and matrix metalloproteinase 2 expression.

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Section: Methodsmentioning

confidence: 99%

C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms

Fu,

Liu,

et al. 2023

Front. Immunol.

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Investigation of anticancer potential of a novel bioactive molecule from Trichosporon asahii VITSTB1 in breast cancer cell lines: an in vitro study

Stany,

Mishra,

Tharani

et al. 2024

Med Oncol

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Enhancement of Cognitive Function by Andrographolide-Loaded Lactose β-Cyclodextrin Nanoparticles: Synthesis, Optimization, and Behavioural Assessment

Paramanick,

Rani,

Singh

et al. 2024

Pharmaceuticals

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This study investigates whether Andrographolide-loaded Lactose β-Cyclodextrin (ALN-βCD) nanoparticles enhance cognitive function, particularly spatial learning and memory. The successful conjugation of lactose to β-cyclodextrin was confirmed via 1H NMR spectroscopy, facilitating neuronal cell entry. The solvent evaporation method was used to create the nanoparticles, which were characterised for particle size, PDI, zeta potential, and drug release. The nanoparticles exhibited a size of 247.9 ± 3.2 nm, a PDI of 0.5 ± 0.02, and a zeta potential of 26.8 ± 2.5 mV. FTIR and TEM analyses, along with in vitro drug release and BBB permeability studies, confirmed their stability and efficacy. Behavioural tests, including the Elevated Plus Maze, Y-Maze, Object Recognition, and Locomotor Activity tests, demonstrated significant improvements in memory, motor coordination, and exploration time in the nanoparticle-treated groups. The group treated with ALN-βCD at a dose of 100 mg/kg/p.o. showed superior cognitive performance compared to the group receiving free andrographolides (AG). Biochemical assays indicated a significant reduction in acetylcholinesterase activity and lipid peroxidation, suggesting increased acetylcholine levels and reduced oxidative stress. Histopathological examination showed improved neuronal function without toxicity. The results showed significant improvements (p < 0.001) in memory and cognitive abilities in experimental animals, highlighting the potential of ALN-βCD nanoparticles as a non-invasive treatment for memory loss. These promising findings warrant further exploration through clinical trials.

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Quantitation of nucleoprotein complexes by UV absorbance and Bradford assay

Cited by 3 publications

References 14 publications

C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms

C-reactive protein deficiency ameliorates experimental abdominal aortic aneurysms

Investigation of anticancer potential of a novel bioactive molecule from Trichosporon asahii VITSTB1 in breast cancer cell lines: an in vitro study

Enhancement of Cognitive Function by Andrographolide-Loaded Lactose β-Cyclodextrin Nanoparticles: Synthesis, Optimization, and Behavioural Assessment

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