Quantitation of progesterone and 20α-dihydroprogesterone in human placenta and fetal membranes

Laatikainen, Timo; Pesonen, K.; Pelkonen, J.

doi:10.1016/0022-4731(81)90024-8

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…P4 modestly attenuated IL-8 and COX-2 mRNA expression but not IL-8 and PGE 2 protein concentration in chorion cells. The local concentration of P4 in fetal membranes may be higher than what has been measured in amniotic fluid [58, 59]. However non-specific steroid effects due to changes in cell membrane fluidity can occur with doses of progesterone in excess of micromolar concentrations [60], making assays using high concentrations difficult to interpret.…”

Section: Discussionmentioning

confidence: 99%

The Role of Progesterone and a Novel Progesterone Receptor, Progesterone Receptor Membrane Component 1, in the Inflammatory Response of Fetal Membranes to Ureaplasma parvum Infection

et al. 2016

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Ureaplasma parvum (U. parvum) is gaining recognition as an important pathogen for chorioamnionitis and preterm premature rupture of membranes. We aimed to investigate the roles of progesterone (P4) and a novel progesterone receptor, progesterone receptor membrane component 1 (PGRMC1), in the response of fetal membranes to U. parvum. Fetal membrane cells (amnion, chorion and decidua) were isolated and confirmed to be free of Mycoplasmataceae. Cells were treated with U. parvum (5x106 CFU), and adherence was quantified by qPCR. Amnion and chorion cells were transfected with scrambled siRNA or validated PGRMC1 siRNA for 72h. Cells were then treated with U. parvum for 4h with or without pretreatment with P4 (10−7 M) or ethanol for 1h. Interleukin-8 (IL-8), matrix metalloproteinase 9 (MMP9) and cyclooxygenase (COX-2) mRNA expression were quantified by qRT-PCR. Culture medium was harvested and analyzed for IL-8 and prostaglandin (PGE2) secretion by ELISA and MMP9 activity by zymography. U. parvum had a mean adherence of 15.0±0.6%, 16.9± 3.7% and 4.7±0.3% in cultured amnion, chorion and decidua cells, respectively. Exposure to U. parvum elicited significant inflammatory responses including induction of IL-8, COX-2, PGE2 and MMP9. A possible role of PGRMC1 was identified in the inhibition of U. parvum-stimulated COX-2 and MMP9 mRNA expression in chorion cells and MMP9 activity in amnion cells. On the other hand, it might enhance the U. parvum-stimulated IL-8 protein secretion in amnion cells. P4, mediated through PGRMC1, significantly inhibited U. Parvum-induced MMP9 mRNA and COX-2 mRNA expression in chorion cells. P4 appeared to attenuate U. parvum induced IL-8 mRNA expression in chorion cells, but this P4 effect might not mediated through PGRMC1. In summary, U. parvum preferentially adheres to and induces inflammatory responses in chorion and amnion cells. P4 and PGRMC1 appear to differentially modulate the inflammatory responses induced by U. parvum among amnion and chorion cells.

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Section: Discussionmentioning

confidence: 99%

The Role of Progesterone and a Novel Progesterone Receptor, Progesterone Receptor Membrane Component 1, in the Inflammatory Response of Fetal Membranes to Ureaplasma parvum Infection

et al. 2016

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Effect of sulprostone on concentrations of progesterone, 20α-dihydroprogesterone, estrone, and estradiol in human placental tissue in the first trimester of pregnancy

et al. 1982

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Placentas were obtained from 35 healthy women whose pregnancy was terminated in the first trimester. Seventeen of them had no prostaglandin medication whereas in 18 women 500 micrograms of sulprostone was administered for cervical softening 12-14 h prior to the termination of pregnancy. Concentrations of progesterone and 20 alpha-dihydroprogesterone were determined from the extract of the placenta by liquid chromatography and those of estrone and estradiol by a radioimmunoassay. Placental progesterone concentration (6,829 +/- 469 ng/g wet weight, mean +/- SEM) was higher in the sulprostone group than in the control group (5,070 +/- 539 ng/g, p less than 0.02), but the mean concentrations of 20 alpha-dihydroprogesterone, estrone and estradiol were similar in both groups. Thus the small dose of sulprostone used in this study did not result in any great changes in placental steroidogenesis which could bring about the initiation of abortion. The increase of placental progesterone may, however, indicate that uterine contractions and impaired uteroplacental blood flow secondarily decreased the direct transfer of steroids from the placenta to the myometrium.

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Labor in humans: 1. progesterone, 20 α-dihydro-progesterone, estrone and 17 β-estradiol in near placental and most distant human amnion and chorion laeve in various stages of labor at term

Cinque

Navarretta

Benedetto

et al. 1986

J Endocrinol Invest

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In some species the onset of labor is regulated by changes in the estrogen/progesterone ratio. The same change in fetal membranes has been suggested to be one of the triggering mechanisms of labor in humans. In order to examine if a gradient in steroid concentration existed in fetal membranes and if changes in concentrations could be observed with the onset and advancing labor, the concentration of progesterone (P), 20 alpha-dihydro-progesterone (20 alpha-OHP), estrone (E1) and 17 beta-estradiol (E2) were determined by specific radioimmunoassay in the near placental and most distant regions of human amnion and chorion laeve obtained at term, before, at the onset and in advanced labor. Both estrogen and progestin concentrations in the chorion were higher than in the amnion. No gradient in the concentration of steroids was found. No statistically significant differences in estrogen and progestin levels were associated with the onset of labor.

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Quantitation of progesterone and 20α-dihydroprogesterone in human placenta and fetal membranes

Cited by 5 publications

References 13 publications

The Role of Progesterone and a Novel Progesterone Receptor, Progesterone Receptor Membrane Component 1, in the Inflammatory Response of Fetal Membranes to Ureaplasma parvum Infection

The Role of Progesterone and a Novel Progesterone Receptor, Progesterone Receptor Membrane Component 1, in the Inflammatory Response of Fetal Membranes to Ureaplasma parvum Infection

Effect of sulprostone on concentrations of progesterone, 20α-dihydroprogesterone, estrone, and estradiol in human placental tissue in the first trimester of pregnancy

Labor in humans: 1. progesterone, 20 α-dihydro-progesterone, estrone and 17 β-estradiol in near placental and most distant human amnion and chorion laeve in various stages of labor at term

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