Quantitation of Tail Lesions in Vaccinia-Infected Mice

Abstract

When mice were infected with vaccinia virus via the tail vein, the number of lesions was influenced by the injection site. A site 3 cm from the base of the tail is of greatest practical value.

Synthesis of 3-deazaneplanocin A, a powerful inhibitor of S-adenosylhomocysteine hydrolase with potent and selective in vitro and in vivo antiviral activities

“…Tailpox Model. This in vivo vaccinia virus model was developed by Boyle et al 22 and further refined by Joshi et al 30 Mice inoculated in the tail vein with virus develop dermal lesions over the entire tail surface. These lesions are enumerated after fluorescence staining and are a function of viral dose, animal weight, and inoculation distance from the base of the tail.22,30 As suggested by Boyle,22 lesion numbers were square root transformed to produce a more normal distribution of data.…”