Quantitation of the soluble receptor of human T lymphocytes for sheep erythrocytes by electroimmunodiffusion in the serum of patients with cancer, uremia and leprosy

Moura, Nayla C.; Longo, I. M.; Bernd, Luiz Antonio Guerra; Mendes, Nelson F.

doi:10.1007/bf01955320

Cited by 10 publications

(7 citation statements)

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“…preseni in normal or uraemic serum, depressed T-cellmediated milogenic responses and impaired E-rosetting. They also reported a qualitative study on the level of soluble E-receptor in the sera of varitius patients [29]. However, no information was given on the biochemical or serologic characteristics of the soluble E-receptor, Owen & Fanger [36] also char:icterized a soluble E-receptor.…”

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confidence: 99%

Quantification of Soluble E‐Receptor in the Serum of Patients with Various Diseases and Its Accompanying in Vitro Immunosuppression in Neoplasia

Lapenson

Morgan

1985

Scand J Immunol

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Evidence in the literature indicates that soluble E-receptor in the serum may modulate T-dependent immune response. We have developed a solid-phase radioimmunoassay to measure the soluble form of E-receptor from various sources. The assay detects solubilized antigens derived from E-receptor-bearing T lymphocytes and not with non-E-receptor-bearing B lymphocytes. The sensitivity limit of the assay is 0.1 ng/ml of purified E-receptor antigen. Using this assay, one can show that the T-cell mitogen phytohaemagglutinin stimulated both lymphocytes and cells of the resting human T-cell lymphoma cell line Jurkat to shed or secrete E-receptor into the culture medium. Results of the radioimmunoassay performed on human sera indicated that some patients with Hodgkin's disease, melanoma, sarcoma, or acute or chronic lymphocytic leukaemia had elevated levels of this antigen in their serum, whereas normal human sera registered lower levels. Elevated levels of the soluble form of E-receptor in the serum were suggestive of an in vitro assessment of their immunosuppressive activity. These results indicate that activation of T-cell immunity in vivo may result in humoral immunosuppression.

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confidence: 99%

Quantification of Soluble E‐Receptor in the Serum of Patients with Various Diseases and Its Accompanying in Vitro Immunosuppression in Neoplasia

Lapenson

Morgan

1985

Scand J Immunol

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“…In cancer patients, we also have observed that when the immune response is restored, Rs decreases to normal levels (15). The role played by Rs in vivo seems to be related to immunoregulation, as demonstrated by us in uremic patients (29).…”

Section: Discussionmentioning

confidence: 86%

“…Rs serum levels are also increased in diseases associated with depression of cell-mediated immunity (15)(16)(17). In cancer patients, we also have observed that when the immune response is restored, Rs decreases to normal levels (15).…”

Section: Discussionmentioning

confidence: 99%

“…With this method we have found abnormally high levels of Rs in the serum from patients with diseases associated with depression of cellmediated immunity such as leukemia, lymphoma, carcinoma, uremia, leprosy, and bone marrow aplasia (15)(16)(17). Increased Rs levels also have been detected by other authors in sera from patients with cancer using anti-CD2 monoclonal antibodies (18).…”

Section: Introductionmentioning

confidence: 99%

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Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Nuñez

Moura

Hernández

et al. 1997

J. Clin. Lab. Anal.

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“…In this study, anti-soluble receptor (anti-Rs) serum was obtained by immunizing sheep with autologous E coated with Rs (14). Previous studies from our group have demonstrated that Rs levels are elevated in cancer, uremia and leprosy patients (7). This anti-Rs serum recognizes molecules of molecular weights of approximately 58,000 and 150,000, the latter being detected in high levels in the serum of patients with cancer and uremia (15).…”

Section: Introductionmentioning

confidence: 99%

Purification of soluble human t lymphocyte receptors for sheep erythrocytes

Itano¹,

Ono²,

Sumigawa³

et al. 1991

Clinical Laboratory Analysis

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Using a polyclonal heterologous anti-soluble E-receptor serum, we identified molecules of molecular weight circa 58,000 and 150,000. The soluble receptor molecule with molecular weight of approximately 58,000 (Rs1) was initially purified from supernatant of heated lymphocytes through chromatography on Sephadex G-200 and/or DEAE-cellulose. The soluble receptor molecule with molecular weight of approximately 150,000 (Rs2) is detected at high levels in the serum of patients with cancer and uremia. Rs1 and Rs2 present in serum from cancer patients were purified by chromatography on Sephadex G-200 and by affinity chromatography using anti-Rs1 IgG. 131I-labelled supernatant of heated lymphocytes binds to sheep erythrocytes and the elution and analysis of the molecules adsorbed showed bands of molecular weights approximately 58,000 and 150,000, confirming the receptor activity of these molecules.

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Quantitation of the soluble receptor of human T lymphocytes for sheep erythrocytes by electroimmunodiffusion in the serum of patients with cancer, uremia and leprosy

Cited by 10 publications

References 10 publications

Quantification of Soluble E‐Receptor in the Serum of Patients with Various Diseases and Its Accompanying in Vitro Immunosuppression in Neoplasia

Quantification of Soluble E‐Receptor in the Serum of Patients with Various Diseases and Its Accompanying in Vitro Immunosuppression in Neoplasia

Quantitation of soluble E-Receptor of T lymphocytes in serum from HIV-1 positive patients

Purification of soluble human t lymphocyte receptors for sheep erythrocytes

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