2015
DOI: 10.1002/cyto.a.22701
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Quantitation of yeast cell–cell fusion using multicolor flow cytometry

Abstract: Mating of haploid Saccharomyces cerevisiae cells of opposite sex provides a powerful model system to study the cell-cell fusion. However, a rapid and standardized method is much needed for quantitative assessment of fusion efficiency. The gold standard method relies on counting mating pairs in fluorescence microscopy images. This current method is limited by expectancy bias and it is time consuming, restricting the number of both cell-cell fusion events and strains that can be analyzed at once. Automatic appro… Show more

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Cited by 3 publications
(3 citation statements)
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“…Cell fusion, somatic hybrids, cytoplasmic hybrids (cybrids), and transferred nuclei. Cell fusion occurring in vivo has been reported in yeast (Salzman et al, 2015), plants (Maruyama et al, 2015), and animals (Zhou and Platt, 2011;Pérez-Vargas et al, 2014…”
Section: Cellular-level Variationmentioning
confidence: 99%
“…Cell fusion, somatic hybrids, cytoplasmic hybrids (cybrids), and transferred nuclei. Cell fusion occurring in vivo has been reported in yeast (Salzman et al, 2015), plants (Maruyama et al, 2015), and animals (Zhou and Platt, 2011;Pérez-Vargas et al, 2014…”
Section: Cellular-level Variationmentioning
confidence: 99%
“…Recent studies involving multispecies cultures utilize fluorescent tags to distinguish different populations. 10 Unfortunately, tracking the dynamics of individual populations even in these tagged multispecies systems is labor intensive without an automated system, thereby constraining experimental progress.…”
mentioning
confidence: 99%
“…However, when there are multiple species in the culture, the turbidity measurement does not provide any information about the population dynamics of each individual species. Recent studies involving multispecies cultures utilize fluorescent tags to distinguish different populations . Unfortunately, tracking the dynamics of individual populations even in these tagged multispecies systems is labor intensive without an automated system, thereby constraining experimental progress.…”
mentioning
confidence: 99%