Quantitative 3D Mapping of Fluidic Temperatures within Microchannel Networks Using Fluorescence Lifetime Imaging

Benninger, Richard K.P.; Koç, Yasemin; Hofmann, Oliver; Requejo-Isidro, José; Neil, Mark A. A.; French, Paul M. W.; deMello, Andrew J.

doi:10.1021/ac051990f

Cited by 121 publications

(105 citation statements)

References 43 publications

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“…The use of FLIM of RhB to measure the temperature of methanol in micro-fluidic channels has been reported previously (Benninger et al 2006) but the low solubility of RhB in water makes it unsuitable for measuring aqueous systems. We have found that Kiton Red (KR), a water-soluble, sulfonated derivative of RhB, shows the same temperature response as the parent fluorophore and is thus ideal for temperature measurement of aqueous solutions.…”

Section: Measurement Of Temperaturementioning

confidence: 99%

Quantitative comparison of thermal and solutal transport in a T-mixer by FLIM and CFD

Mendels

Graham

Magennis

et al. 2008

Microfluid Nanofluid

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The development and adoption of lab-on-a-chip and micro-TAS (total analysis system) techniques requires not only the solving of design and manufacturing issues, but also the introduction of reliable and quantitative methods of analysis. In this work, two complementary tools are applied to the study of thermal and solutal transport in liquids. The experimental determination of the concentration of water in a water-methanol mixture and of the temperature of water in a microfluidic T-mixer are achieved by means of fluorescence lifetime imaging microscopy (FLIM). The results are compared to those of finite volume simulations based on tabulated properties and well-established correlations for the fluid properties. The good correlation between experimental and modelled results demonstrate without ambiguity that (1) the T-mixer is an adiabatic system within the conditions, fluids and flow rates used in this study, (2) buoyancy effects influence the mixing of liquids of different densities at moderate flow rates (Reynolds number Re ( 10 -2 ), and (3) the combination of FLIM and computational fluid dynamics has the potential to be used to measure the thermal and solutal diffusion coefficients of fluids for a range of temperatures and concentrations in one single experiment. As such, it represents a first step towards the full-field monitoring of both the extent and the kinetics of a chemical reaction.

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Section: Measurement Of Temperaturementioning

confidence: 99%

Quantitative comparison of thermal and solutal transport in a T-mixer by FLIM and CFD

Mendels

Graham

Magennis

et al. 2008

Microfluid Nanofluid

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“…The lifetime-based techniques generally require more complex diagnostic equipment compared to intensity-based measurements, though they are less sensitive to environmental effects. Besides the temperature measurements, the fluorescent techniques also were employed in studies of flows inside micro channels in lab-on-chip applications [9,10], in microviscosity investigations [11,12], air flow sensing [13,14], and as pressure measurements [15]. In order to achieve stable and reliable results, the certain balance between isolation and overlap of the probe with the environment is desired.…”

Section: Introductionmentioning

confidence: 99%

Temperature and Phase Transition Sensing in Liquids with Fluorescent Probes

et al. 2017

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Local environment of fluorescent dyes could strongly affects emission dynamics of the latter. In particular, both signal intensities and emission lifetimes are highly sensitive to solvent temperatures. Here, temperature-dependent behavior Rhodamine B fluorescence in water and ethanol solutions was experimentally investigated. Phase transition point between liquid water and ice was shown to have a dramatic impact on both in intensity (30-fold drop) and in lifetime (from 2.68 ns down to 0.13 ns) of the dye luminescence along with the shift of spectral maxima from 590 to 625 nm. At the same time, use of ethanol as solvent does not lead to any similar behavior. The reported results and approaches enable further investigations of dye-solvent interactions and studies of physical properties at phase transition points.

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“…Fluorescence lifetime imaging (FLIM) [8][9][10] is an increasingly utilized tool in biological research that can provide robust contrast between different fluorescent proteins/species and can also yield quantitative information concerning the local fluorophore environment, e.g. pH, temperature, refractive index [11][12][13], etc., including protein-protein interactions via Fö rster Resonance Energy Transfer (FRET) [14,15]. In this paper we describe a FLIM-OPT system which can reconstruct the 3-D fluorescence lifetime distribution of volumetric samples up to several mm in size, and report its application to imaging mouse embryos.…”

Section: Biophotonicsmentioning

confidence: 99%

Fluorescence lifetime optical projection tomography

McGinty¹,

Tahir

Laine

et al. 2008

Journal of Biophotonics

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One of the recent trends in biological research has been a progression from average measurements on cell populations (e.g. homogenous well-plate assays) to fixed and live cell imaging for sub-cellular spatial localization, study of metabolic signals and spatiotemporally resolved dynamics. There is now an increasing appreciation that in vitro cell mono-layers may exhibit non-physiological behaviour due to the artificially planar environment. This has led to the development of 3-D cell cultures BIOPHOTONICSWe describe a quantitative fluorescence projection tomography technique which measures the 3-D fluorescence lifetime distribution in optically cleared specimens up 1 cm in diameter. This is achieved by acquiring a series of wide-field time-gated images at different relative time delays with respect to a train of excitation pulses, at a number of projection angles. For each time delay, the 3-D time-gated intensity distribution is reconstructed using a filtered back projection algorithm and the fluorescence lifetime subsequently determined for each reconstructed horizontal plane by iterative fitting to a monoexponential decay. Due to its inherently ratiometric nature, fluorescence lifetime is robust against intensity based artefacts as well as producing a quantitative measure of the fluorescence signal. We present a 3-D fluorescence lifetime reconstruction of a mouse embryo labelled with an alexa-488 conjugated antibody targeted to the neurofilament, which clearly differentiates between the extrinsic label and the autofluorescence, particularly from the heart and dorsal aorta.

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Quantitative 3D Mapping of Fluidic Temperatures within Microchannel Networks Using Fluorescence Lifetime Imaging

Cited by 121 publications

References 43 publications

Quantitative comparison of thermal and solutal transport in a T-mixer by FLIM and CFD

Quantitative comparison of thermal and solutal transport in a T-mixer by FLIM and CFD

Temperature and Phase Transition Sensing in Liquids with Fluorescent Probes

Fluorescence lifetime optical projection tomography

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