Quantitative absorption imaging of red blood cells to determine physical and mechanical properties

Paul, Ratul; Zhou, Yuyuan; Nikfar, Mehdi; Razizadeh, Meghdad; Liu, Yaling

doi:10.1039/d0ra05421f

Cited by 12 publications

(10 citation statements)

References 96 publications

(129 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spectral imaging systems are either multispectral, hyper-spectral, or ultra-spectral according to the biochemical components [58]. Studies indicate that RBCs' physical and mechanical properties such as hemoglobin concentration, total volume, and membrane characteristics can be effectively analyzed using the light absorption images as shown in Figure 5A [59]. Such measurements are applicable for clinical evaluations, such as the detection of malaria through a point of care (LOC) device [60] as showed in Figure 5B.…”

Section: Spectroscopic Analysis Of Single Rbcsmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

View full text Add to dashboard Cite

The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

show abstract

Section: Spectroscopic Analysis Of Single Rbcsmentioning

confidence: 99%

“…State-of-the-art [58], RBC physics and mechanics [59,61], POC malaria diagnosis [60], aggregation dynamics [63], hybrid analytical platforms [62] Low processing speeds, limitations in wavelength and challenges due to the variation of device elements.…”

Section: Spectral Imagingmentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It was not a generalized study with various bloods collected from patients. In the near future, it will be necessary to validate the performance of the present method with clinical disease bloods [2,13,35,36]. Furthermore, to improve the portability of the present experimental setup, the two syringe pumps will be replaced by portable micropumps [37][38][39].…”

Section: Quantitative Comparison Between the Proposed And Conventiona...mentioning

confidence: 99%

Contributions of Red Blood Cell Sedimentation in a Driving Syringe to Blood Flow in Capillary Channels

Kang

2022

Micromachines

View full text Add to dashboard Cite

The erythrocyte sedimentation rate (ESR), which has been commonly used to detect physiological and pathological diseases in clinical settings, has been quantified using an interface in a vertical tube. However, previous methods do not provide biophysical information on blood during the ESR test. Therefore, it is necessary to quantify the individual contributions in terms of viscosity and pressure. In this study, to quantify RBC sedimentation, the image intensity (Ib) and interface (β) were obtained by analyzing the blood flow in the microfluidic channels. Based on threshold image intensity, the corresponding interfaces of RBCs (Ib > 0.15) and diluent (Ib < 0.15) were employed to obtain the viscosities (µb, µ0) and junction pressures (Pb, P0). Two coefficients (CH1, CH2) obtained from the empirical formulas (µb = µ0 [1 + CH1], Pb = P0 [1 + CH2]) were calculated to quantify RBC sedimentation. The present method was then adopted to detect differences in RBC sedimentation for various suspended blood samples (healthy RBCs suspended in dextran solutions or plasma). Based on the experimental results, four parameters (µ0, P0, CH1, and CH2) are considered to be effective for quantifying the contributions of the hematocrit and diluent. Two coefficients exhibited more consistent trends than the conventional ESR method. In conclusion, the proposed method can effectively detect RBC sedimentation.

show abstract

“…Red blood cells (RBCs) are highly deformable. When not in flow, the majority of human RBCs are shaped like biconcave disks with 6–8 μm diameter and 2.5 μm thickness . While passing through the blood vessels, RBCs undergo shape transitions from discocyte to parachute, jellyfish, or torpedo shape depending on the vessel diameter and flow rate .…”

Section: Introductionmentioning

confidence: 99%

“…When not in flow, the majority of human RBCs are shaped like biconcave disks with 6–8 μm diameter and 2.5 μm thickness. 1 While passing through the blood vessels, RBCs undergo shape transitions from discocyte to parachute, jellyfish, or torpedo shape depending on the vessel diameter and flow rate. 2 These shape changes enable the cells to pass through the micron-sized capillary vessels smaller in size than the RBC diameter without damage or membrane rupture, supporting the effective transport of gases (mainly oxygen and carbon dioxide) between the lungs and the peripheral tissues, capillary vessels, and peripheral tissues.…”

Section: Introductionmentioning

confidence: 99%

Simple Assessment of Red Blood Cell Deformability Using Blood Pressure in Capillary Channels for Effective Detection of Subpopulations in Red Blood Cells

et al. 2022

View full text Add to dashboard Cite

Assessment of red blood cell (RBC) deformability as a biomarker requires expensive equipment to induce and monitor deformation. In this study, we present a simple method for quantifying RBC deformability. We designed a microfluidic channel consisting of a micropillar channel and a coflowing channel connected in series. When blood (loading volume = 100 μL) was injected continuously into the device under constant pressure (1 bar), we monitored the boundary position of the blood and the reference flow in the coflowing channel. A decrease in the deformability of RBCs results in a growing pressure drop in the micropillar channel, which is mirrored by a decrease in blood pressure in the coflowing channel. Analysis of this temporal variation in blood pressure allowed us to define the clogging index (CI) as a new marker of RBC deformability. As a result of the analytical study and numerical simulation, we have demonstrated that the coflowing channel may serve as a pressure sensor that allows the measurement of blood pressure with accuracy. We have shown experimentally that a higher hematocrit level (i.e., more than 40%) does not have a substantial influence on CI. The CI tended to increase to a higher degree in glutaraldehyde-treated hardened RBCs. Furthermore, we were able to resolve the difference in deformability of RBCs between two different RBC density subfractions in human blood. In summary, our approach using CI provides reliable information on the deformability of RBCs, which is comparable to the readouts obtained by ektacytometry. We believe that our microfluidic device would be a useful tool for evaluating the deformability of RBCs, which does not require expensive instruments (e.g., high-speed camera) or time-consuming micro-PIV analysis.

show abstract

Quantitative absorption imaging of red blood cells to determine physical and mechanical properties

Abstract: The constant thickness in the microfluidic channel is used for controlled absorption of red and blue light to measure red blood cell hemoglobin and height mapping. High speed recording of the height mapping provides us the membrane fluctuation.

Cited by 12 publications

References 96 publications

Advances in Microfluidics for Single Red Blood Cell Analysis

Advances in Microfluidics for Single Red Blood Cell Analysis

Contributions of Red Blood Cell Sedimentation in a Driving Syringe to Blood Flow in Capillary Channels

Simple Assessment of Red Blood Cell Deformability Using Blood Pressure in Capillary Channels for Effective Detection of Subpopulations in Red Blood Cells

Contact Info

Product

Resources

About