Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

Banerjee, Sourav; Gummadidala, Phani M.; Rima, Rowshan Ara; Ahmed, Raiz U.; Kenne, Gabriel J.; Mitra, Chandrani; Gomaa, Ola M.; Hill, James N.; McFadden, Sandra L.; Banaszek, Nora; Fayad, Raja; Terejanu, Gabriel; Chanda, Anindya

doi:10.1016/j.fgb.2014.10.006

Cited by 8 publications

(11 citation statements)

References 28 publications

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“…This can be explained by the fact that wavelength increased with depth and hence reduced pressure was required to wrinkle the media. Our results are also in line with our previous observations14 that the strength of the fungus colony decreases with the depth, possibly due to the significant decrease in number of active hyphae compared to the surface.…”

Section: Resultssupporting

confidence: 93%

“…1, we incorporated in our calculations the following values: depth of the agar media, H = 2.14 ± 0.08 mm, Elastic Modulus, E, of the Agar media = 700 ± 21 kPa, that was determined from the wave velocity in the medium1431, and Poisson ratio, 0.49. Figure 4 depicts the mean value of the pressure distribution across the depth obtained from 12 specimens.…”

Section: Resultsmentioning

confidence: 99%

“…To address the critical need for quantitative tools for measuring fungal invasion, we have recently developed a methodology for 3D tomography of a growing fungal colony14. We have already demonstrated in Aspergillus parasiticus, an aflatoxin producing plant pathogen, that our method which we call Q uantitative A cosutic C ontrast T omography (Q-ACT), can provide physical strength profiles (viscoelastic parameters) as well as hyphal architecture at multiple scales in a growing colony14.…”

mentioning

confidence: 99%

“…We have already demonstrated in Aspergillus parasiticus, an aflatoxin producing plant pathogen, that our method which we call Q uantitative A cosutic C ontrast T omography (Q-ACT), can provide physical strength profiles (viscoelastic parameters) as well as hyphal architecture at multiple scales in a growing colony14. In this study we have expanded the functionality of Q-ACT by investigating the physical changes that occur within the substrate beneath and around the fungal colony.…”

mentioning

confidence: 99%

“…We reason that such an orchestrated pressure distribution on the substrate will depend on the mycelial growth pattern and the physical parameters that determine the strength of the fungal hyphae. Interestingly, our recent Q-ACT based studies already demonstrated that physical strength profiles within the mycelia correlate inherently with secondary metabolism14 suggesting that pressure profiles generated by fungal colonies are critical determinants of their metabolic state as well as their invasiveness into the substrate. Currently, very little is known about relation between the generation of the pressure by a fungal colony on the substrate and the collective physical behavior of the multicellular system of the fungal colony.…”

mentioning

confidence: 99%

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Subsurface pressure profiling: a novel mathematical paradigm for computing colony pressures on substrate during fungal infections

Patra

Banerjee

Terejanu

et al. 2015

Sci Rep

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Colony expansion is an essential feature of fungal infections. Although mechanisms that regulate hyphal forces on the substrate during expansion have been reported previously, there is a critical need of a methodology that can compute the pressure profiles exerted by fungi on substrates during expansion; this will facilitate the validation of therapeutic efficacy of novel antifungals. Here, we introduce an analytical decoding method based on Biot’s incremental stress model, which was used to map the pressure distribution from an expanding mycelium of a popular plant pathogen, Aspergillus parasiticus. Using our recently developed Quantitative acoustic contrast tomography (Q-ACT) we detected that the mycelial growth on the solid agar created multiple surface and subsurface wrinkles with varying wavelengths across the depth of substrate that were computable with acousto-ultrasonic waves between 50 MHz–175 MHz. We derive here the fundamental correlation between these wrinkle wavelengths and the pressure distribution on the colony subsurface. Using our correlation we show that A. parasiticus can exert pressure as high as 300 KPa on the surface of a standard agar growth medium. The study provides a novel mathematical foundation for quantifying fungal pressures on substrate during hyphal invasions under normal and pathophysiological growth conditions.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Subsurface pressure profiling: a novel mathematical paradigm for computing colony pressures on substrate during fungal infections

Patra

Banerjee

Terejanu

et al. 2015

Sci Rep

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Mycoremediation with mycotoxin producers: a critical perspective

Chanda

Gummadidala

Gomaa

2015

Appl Microbiol Biotechnol

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Filamentous fungi that produce mycotoxins also demonstrate the ability to degrade a wide variety of naturally occurring and anthropogenically generated hazardous wastes. Hence, these are emerging as excellent candidates for bioremediation. Their mycelia exhibit the robustness of adapting to highly restrictive environmental conditions often experienced in the presence of persistent pollutants, which makes them more useful compared to other microbes. However, it now appears that several regulatory factors that govern mycotoxin synthesis in these toxigenic strains also regulate their bioremediation abilities. To this end, mycoremediation and mycotoxin synthesis have been thoroughly but independently investigated; hence, much less is understood about the overlaps between the two processes. This review aims to shed light on this critical knowledge gap and provide some useful insights into the future research that might overcome the challenges associated with these shared regulatory modules. This will enable the harnessing of the full potential of mycoremediation by minimizing mycotoxin contamination.

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Processing Techniques or Mycotoxins—A Balancing Act of Food Safety and Preservation

Pal

Pandey

Sen

2019

Preservatives and Preservation Approaches in Beverages

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Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

Cited by 8 publications

References 28 publications

Subsurface pressure profiling: a novel mathematical paradigm for computing colony pressures on substrate during fungal infections

Subsurface pressure profiling: a novel mathematical paradigm for computing colony pressures on substrate during fungal infections

Mycoremediation with mycotoxin producers: a critical perspective

Processing Techniques or Mycotoxins—A Balancing Act of Food Safety and Preservation

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