Quantitative alterations in bovine milk proteome from healthy, subclinical and clinical mastitis during S. aureus infection

Maity, Sudipa; Das, Debangshu Narayan; Ambatipudi, Kiran

doi:10.1016/j.jprot.2020.103815

Cited by 25 publications

(24 citation statements)

References 78 publications

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“…The identification of protein repertoires in the milk, serum, and mammary glands of cows suffering from CM is ideal for identifying potential biomarkers for the development of rapid diagnostic tests [ 27 , 31 , 32 ]. This approach has been used to screen markers, such as Toll-like receptors, cathelicidins, cathepsins, and apoptotic factors [ 27 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Zhang

Lin

Bai

et al. 2022

Animals

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H2S plays an important role in various inflammatory diseases. However, the role of H2S and synthetic enzymes in Holstein cows with CM is unknown. The aim of this study was to identify DEPs associated with sulfide metabolism and further investigate their roles in dairy cows with CM. From 3739 DEPs generated by data-independent acquisition proteomics, we identified a total of 17 DEPs included in 44 GO terms and five KEGG pathways related to sulfide metabolism, including CTH and cystathionine-β-synthase (CBS). Immunohistochemical and immunofluorescence staining results showed that CTH and CBS proteins were present mainly in the cytoplasm of mammary epithelial cells. Endogenous H2S production in the serum of the CM group was significantly lower than that of the healthy Holstein cows. CTH and CBS mRNA and protein levels in the mammary glands of the CM group were significantly downregulated compared to those of the healthy group. These results indicate that CTH and H2S were correlated with the occurrence and development of CM in Holstein cows, which provides important insights into the function and regulatory mechanism of CTH/H2S in Holstein cows.

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Section: Discussionmentioning

confidence: 99%

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Zhang

Lin

Bai

et al. 2022

Animals

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“…This subunit associates with the β1 subunit to form a fibronectin and fibrinogen receptor. Both fibronectin and fibrinogen can mediate adhesion of S. aureus to host cells [ 49 ], and increase of fibronectin levels in whey of Holstein-Friesian cows is associated with mastitis progression [ 50 ]. The integrin α9 subunit encoded by the ITGA9 gene, when bound to the β1 chain, forms an integrin that is a receptor for vascular cell adhesion molecule 1 (VCAM1), cytotactin and osteopontin, all of which are involved in immune response.…”

Section: Discussionmentioning

confidence: 99%

Bovine milk somatic cell transcriptomic response to Staphylococcus aureus is dependent on strain genotype

et al. 2021

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Background Mastitis is an economically important disease of dairy cows with Staphylococcus aureus a major cause worldwide. Challenge of Holstein-Friesian cows demonstrated that S. aureus strain MOK124, which belongs to Clonal Complex (CC)151, caused clinical mastitis, while strain MOK023, belonging to CC97, caused mild or subclinical mastitis. The aim of this study was to elucidate the molecular mechanisms of the host immune response utilising a transcriptomic approach. Milk somatic cells were collected from cows infected with either S. aureus MOK023 or MOK124 at 0, 24, 48, 72 and 168 h post-infection (hpi) and analysed for differentially expressed (DE) genes in response to each strain. Results In response to MOK023, 1278, 2278, 1986 and 1750 DE genes were found at 24, 48, 72 and 168 hpi, respectively, while 2293, 1979, 1428 and 1544 DE genes were found in response to MOK124 at those time points. Genes involved in milk production (CSN1, CSN10, CSN1S2, CSN2, a-LACTA and PRLR) were downregulated in response to both strains, with a more pronounced decrease in the MOK124 group. Immune response pathways such as NF-κB and TNF signalling were overrepresented in response to both strains at 24 hpi. These immune pathways continued to be overrepresented in the MOK023 group at 48 and 72 hpi, while the Hippo signalling, extracellular matrix interaction (ECM) and tight junction pathways were overrepresented in the MOK124 group between 48 and 168 hpi. Cellular composition analysis demonstrated that a neutrophil response was predominant in response to MOK124, while M1 macrophages were the main milk cell type post-infection in the MOK023 group. Conclusions A switch from immune response pathways to pathways involved in maintaining the integrity of the epithelial cell layer was observed in the MOK124 group from 48 hpi, which coincided with the occurrence of clinical signs in the infected animals. The higher proportion of M1 macrophages in the MOK023 group and lack of substantial neutrophil recruitment in response to MOK023 may indicate immune evasion by this strain. The results of this study highlight that the somatic cell transcriptomic response to S. aureus is dependent on the genotype of the infecting strain.

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“…Due to the complex pathogenesis and low cure rate, the incidence of dairy cow mastitis could be as high as 50~70% in China, and the treatment cost is nearly 200 million yuan every year [ 1 ]. Many researchers are devoted to investigating the underlying biological pathogenesis of mastitis to diagnose and manage this disease [ 2 , 3 , 4 ]. Pathogen infection is the main cause of mastitis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

et al. 2022

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Interleukin-6 (IL-6) is generally used as a biomarker for the evaluation of inflammatory infection in humans and animals. However, there is no approach for the on-site and rapid detection of IL-6 for the monitoring of mastitis in dairy farm scenarios. A rapid and highly sensitive surface enhanced Raman scattering (SERS) immunofiltration assay (IFA) for IL-6 detection was developed in the present study. In this assay, a high sensitivity gold core silver shell SERS nanotag with Raman molecule 4-mercaptobenzoic acid (4-MBA) embedded into the gap was fabricated for labelling. Through the immuno-specific combination of the antigen and antibody, antibody conjugated SERS nanotags were captured on the test zone, which facilitated the SERS measurement. The quantitation of IL-6 was performed by the readout Raman signal in the test region. The results showed that the detection limit (LOD) of IL-6 in milk was 0.35 pg mL−1, which was far below the threshold value of 254.32 pg mL−1. The recovery of the spiking experiment was 87.0-102.7%, with coefficients of variation below 9.0% demonstrating high assay accuracy and precision. We believe the immunosensor developed in the current study could be a promising tool for the rapid assessment of mastitis by detecting milk IL-6 in dairy cows. Moreover, this versatile immunosensor could also be applied for the detection of a wide range of analytes in dairy cow healthy monitoring.

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Quantitative alterations in bovine milk proteome from healthy, subclinical and clinical mastitis during S. aureus infection

Cited by 25 publications

References 78 publications

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Sulfur Amino Acid Metabolism and the Role of Endogenous Cystathionine-γ-lyase/H2S in Holstein Cows with Clinical Mastitis

Bovine milk somatic cell transcriptomic response to Staphylococcus aureus is dependent on strain genotype

Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

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