Quantitative analysis and recovery optimisation of flavonoids and anthocyanins in sugar-making process of sugarcane industry

Li, Xinying; Song, Hang; Yao, Shun; Jia, Chunmei; Yang, Yingying; Zhu, Weixia

doi:10.1016/j.foodchem.2010.08.053

Cited by 25 publications

(23 citation statements)

References 18 publications

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“…The solvents were evaporated to dryness under vacuum and the residue was diluted in distilled water. After separating the precipitate by filtration; the remaining solution was further separated on an adsorptive resin column (X-5; Nankai University, Tianjin, China) [15] and eluted with water, 20%, 30%, 40%, 50%, 60%, 80% and 95% EtOH to produce eight fractions. The 30%-EtOH fraction was dried under vacuum, recrystallized from EtOH, and a red powder was obtained containing the ALB.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Anthocyanins from Lingonberry on Radiation-induced Damages

Fan

Wang

Zuo

et al. 2012

IJERPH

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There is a growing concern about the serious harm of radioactive materials, which are widely used in energy production, scientific research, medicine, industry and other areas. In recent years, owing to the great side effects of anti-radiation drugs, research on the radiation protectants has gradually expanded from the previous chemicals to the use of natural anti-radiation drugs and functional foods. Some reports have confirmed that anthocyanins are good antioxidants, which can effectively eliminate free radicals, but studies on the immunoregulatory and anti-radiation effects of anthocyanins from lingonberry (ALB) are less reported. In this experiment, mice were given orally once daily for 14 consecutive days before exposure to 6 Gy of gamma-radiation and were sacrificed on the 7th day post-irradiation. The results showed that the selected dose of extract did not lead to acute toxicity in mice; while groups given anthocyanins orally were significantly better than radiation control group according to blood analysis; pretreatment of anthocyanins significantly (p < 0.05) enhanced the thymus and spleen indices and spleen cell survival compared to the irradiation control group. Pretreatment with anthocyanins before irradiation significantly reduced the numbers of micronuclei (MN) in bone marrow polychromatic erythrocytes (PCEs). These findings indicate that anthocyanins have immunostimulatory potential against immunosuppression induced by the radiation.

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Section: Methodsmentioning

confidence: 99%

Protective Effect of Anthocyanins from Lingonberry on Radiation-induced Damages

Fan

Wang

Zuo

et al. 2012

IJERPH

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“…The MC was determined by weight difference after vacuum drying for 24 h (65 ± 2 °C, 13 kPa) [29]. The TSS quantification was made by measuring the refractive index at 20 °C using a refractometer with electronic temperature control and conversion to Brix degrees values (Index Instruments Ltd. Model TCR [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. The pH readings were done directly on the vinasse samples and diluted molasses with a digital pH-meter (Hach ® Model HQ11D).…”

Section: Physicochemical Characterization Of By-productsmentioning

confidence: 99%

“…Although multiple studies have explored some of the products and by-products derived from sugarcane processing as a source of bioactive antioxidant compounds [4,15,[17][18][19][20][21][22][23], the present study focuses on molasses and even more in vinasses since the latter are the less explored byproduct in the sugarcane industry in terms of its potential biological activity. Among the molecules that have been isolated from different matrices from sugarcane processing it is possible to find several compounds such as apigenin, luteolin, tricine, quercetin, and gallic, caffeic, chlorogenic, ferulic, vanillic, and syringic acids, among others [4,15,17,18,21,24].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Estimation of Total Phenolic Content and Antioxidant Capacity of Molasses and Vinasses Generated from the Sugarcane Industry

Molina-Cortés

Sánchez-Motta

Tobar-Tosse

et al. 2019

Waste Biomass Valor

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To identify a possible use for some agroindustrial wastes generated from the sugarcane industry, we evaluated the antioxidant capacity of B and C molasses and vinasses from the sugar and bioethanol production processes. Molasses and vinasses were characterized by physicochemical methods. Subsequently, the samples were diluted in distilled water at five concentrations to obtain aqueous extracts. Total phenolic content (TPC) of the samples was determined using a spectrophotometric method and was expressed in mg equivalents of gallic acid. The antioxidant capacity of each sample was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS (2,2-azino-bis-[3-ethylbenzothiazoline-6-sulfonic acid]) methods, as well as the ferric-reducing power in FRAP (Ferric-reducing Antioxidant Power) assay. We found that, both for TPC and antioxidant capacity, the vinasses showed significantly higher values than the B and C molasses. These results showed a strong correlation between TPC and antioxidant capacity and revealed a remarkable increase in TPC and total antioxidant agents present in the extracts throughout the sugarcane transformation process. These findings allowed identifying vinasses as the by-product with the best antioxidant properties. Our work constitutes a first step in the study of molasses and vinasses as a promising antioxidant agent and as a novel resource to test in proliferative trials in cellular systems in vitro.

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“…A. arvensis b-glucosidase immobilized on SiO 2 nanoparticles exhibited 95 % of its original activity even after the 25th cycle of reuse (Singh et al 2011). Thus, after immobilization b-glucosidase could be used for multiple cycles of hydrolysis without any significant loss in its activity (Su et al 2010;Li et al 2011;Chang and Juang 2007). Such improvement in overall performance i.e., high stability and reusability makes the immobilized b-glucosidase useful for food and flavoring industries.…”

Section: Storage Stability and Reusability Of Immobilized B-glucosidasementioning

confidence: 99%

Immobilization of β-glucosidase onto silicon oxide nanoparticles and augment of phenolics in sugarcane juice

2016

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Purified b-glucosidase was immobilized on SiO 2 nanoparticles with 52 % efficiency and 14.1 % yield. It had a temperature optima at 60°C and pH optima of 5.0. Immobilized enzyme was fairly stable at 60-70°C. After immobilization, the K m value of b-glucosidase for p-nitrophenyl-b-D-glucopyranoside (pNPG) increased from 0.9 to 1.074 mM and V max decreased from 3.5 to 1.513 U/mg. The immobilized enzyme showed improved storage stability at temperature 4 and 25°C and was reusable for up to ten cycles with 70 % residual activity in pNPG and 60 % residual activity in sugarcane juice treatment. Sugarcane juice density, viscosity; surface tension etc. changed after treatment with immobilized b-glucosidase. b-Glucosidase treated sugarcane juice showed higher phenolics than untreated sugarcane juice. Caffeic acid which was absent in juice, was detected in b-glucosidase treated juice at a concentration of about 1 mg/L.

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Quantitative analysis and recovery optimisation of flavonoids and anthocyanins in sugar-making process of sugarcane industry

Cited by 25 publications

References 18 publications

Protective Effect of Anthocyanins from Lingonberry on Radiation-induced Damages

Protective Effect of Anthocyanins from Lingonberry on Radiation-induced Damages

Spectrophotometric Estimation of Total Phenolic Content and Antioxidant Capacity of Molasses and Vinasses Generated from the Sugarcane Industry

Immobilization of β-glucosidase onto silicon oxide nanoparticles and augment of phenolics in sugarcane juice

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