1961
DOI: 10.1021/ac60176a054
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Quantitative Analysis by an Automatic Potentiometric Reaction Rate Method. Specific Enzymatic Determination of Glucose

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1963
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Cited by 91 publications
(41 citation statements)
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“…The typical one is an amperometric glucose biosensor. Most previous studies on this subject involved the use of glucose oxidase (GOx) based on its direct electron transfer or its catalytic activity to produce readily-detectable H 2 O 2 by the reduction of O 2 by glucose [2][3][4][5][6]. This type of biosensor usually shows good selectivity and high sensitivity, but, its long-term stability is poor due to the use of GOx.…”
Section: Introductionmentioning
confidence: 99%
“…The typical one is an amperometric glucose biosensor. Most previous studies on this subject involved the use of glucose oxidase (GOx) based on its direct electron transfer or its catalytic activity to produce readily-detectable H 2 O 2 by the reduction of O 2 by glucose [2][3][4][5][6]. This type of biosensor usually shows good selectivity and high sensitivity, but, its long-term stability is poor due to the use of GOx.…”
Section: Introductionmentioning
confidence: 99%
“…By using a different fast coupling reaction, as illustrated in Equation 3, it was possible to determine the rate of change of H 2 O 2 31 Molybdate 20H" (3) Catalyst by following the I 3 " using potentiometric, 17 spectrophotometric, 18 and amperometric 19 encoding (transducer) systems, and thus eliminate the troublesome peroxidase enzyme.…”
Section: Encoding Indirectly Via a Coupling Reactionmentioning
confidence: 99%
“…An operational amplifier (OA) is wired as a differentiator (Figure 17), and the voltage from a suitable transducer is applied to the input. The relationship between the input voltage ( e jn ) and the output voltage (e Q ) is given as: (17) The derivative method best characterizes the kinetics of a given reaction since it provides the instantaneous value of the tangent at one point on the rate curve. Paradoxically, this is a factor which limits the method since, at the required sensitivity, the rate curves are accompanied by noise.…”
Section: Manual Data Manipulationmentioning
confidence: 99%
“…Nucleic acid detection using hybridization techniques has received significant attention due to its valuable applications in clinical diagnostics, national defense and forensics . Inspired by the undoubted success of the blood glucose meter, electrochemical methods have been explored as a valuable approach for nucleic acid detection due to the potential for on‐site testing while offering a fast, simple and inexpensive analysis . Fan and colleagues initiated the field of electrochemical nucleic acid analysis by introducing a DNA stem‐loop (SL) probe conjugated with a redox marker .…”
Section: Introductionmentioning
confidence: 99%