Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Hoffmann, Georg F.; Aramaki, Shuichi; Blum-Hoffmann, Ellen; Nyhan, William L.; Sweetman, Larry

doi:10.1093/clinchem/35.4.587

Cited by 191 publications

(55 citation statements)

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“…Reference b Organic acid assays were performed by gas chromatography-mass spectrometry in accordance with the methodology described by Hoffman et al 5 c Reference intervals have not yet been established for feline organic acid concentrations. Laboratory reference intervals for canine urine organic acid concentrations are presented in this table as an estimate for domestic animals.…”

Section: Pretreatmentmentioning

confidence: 99%

d-Lactic Acidosis Secondary to Exocrine Pancreatic Insufficiency in a Cat

et al. 2005

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A n indoor, 2-year-old, spayed female domestic cat was referred to the University of Missouri Veterinary Medical Teaching Hospital (UMC-VMTH) for evaluation of weight loss and polyphagia of 1-year duration, with recent episodes of generalized weakness and lethargy. These episodes occurred approximately once per month over the past 4 months. The cat produced frequent stools, which were occasionally poorly formed. A CBC and serum biochemistry were performed 1 and 5 months before referral, and leukopenia (1,200/L; reference interval, 3,500-16,000/L; no differential provided), hypocholesterolemia (64 mg/dL and 46 mg/dL; reference interval, 75-220 mg/ dL), and hypotriglyceridemia (22 mg/dL and 16 mg/dL; reference interval, 26-160 mg/dL) were identified. The most recent serum biochemistry results disclosed mild hypercalcemia (11.3 mg/dL; reference interval, 8.2-10.8 mg/ dL) and hypernatremia (163 mg/dL; reference interval, 145-158 mg/dL). Antibody titers for Toxoplasma were consistent with past exposure (immunoglobulin M [IgM] negative; IgG 1:256).The cat did not improve with clindamycin therapy (11 mg/kg PO q12h), prednisone (2 mg/kg q24h), and vitamin supplements (dosage unknown). During recent episodes of severe weakness, supportive care with IV lactated Ringer's solution, 5% dextrose, vitamin B complex, penicillin G benzathine, and dexamethasone (dosages unknown) improved the generalized weakness, but did not affect appetite or weight loss. The cat was referred to the UMC-VMTH for further diagnostic testing 12 days after the last episode.The cat was emaciated (body condition score, 2 out of 9; weight, 2.85 kg), and had an unkempt hair coat. Vital signs and hydration were normal. Mentation appeared normal at admission, but gait showed subtle evidence of weakness and ataxia with occasional crossing-over of the paws in all 4 limbs. Incomplete closure of the palpebral fissure

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Section: Pretreatmentmentioning

confidence: 99%

d-Lactic Acidosis Secondary to Exocrine Pancreatic Insufficiency in a Cat

et al. 2005

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“…To investigate the possibility of a metabolic disorder, a urine sample (collected by cystocentesis before thiamine treatment) was submitted for evaluation of urinary organic and amino acid concentrations. Organic acids were quantified by gas chromatography-mass spectrometry as described by Hoffman et al 1 Results were compared to canine reference values obtained from 10 normal adult dogs previously evaluated by the laboratory. Biochemical abnormalities included lactic aciduria (1,423 mmol/mol creatinine; controls, 10 and 240 mmol/mol creatinine), 2-hydroxyisovaleric aciduria (83 mmol/mol creatinine; controls, nondetectable), and 2-hydroxyadipic aciduria (58 mmol/ mol creatinine; controls, nondetectable).…”

mentioning

confidence: 99%

Thiamine Deficiency in a Dog: Clinical, Clinicopathologic, and Magnetic Resonance Imaging Findings

Garosi

Dennis

Platt

et al. 2003

Veterinary Internal Medicne

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“…Plasma and urine were collected antemortem from dog 2 and submitted to the Comparative Neuromuscular Laboratory d for plasma amino acid and urine organic acid analysis. Urinary organic acids were quantified by gas chromatography-mass spectrometry, as described by Hoffman et al, 32 and plasma amino acids were quantified by automated column chromatography, as described by Spackman et al 33 Plasma, urine, and muscle carnitine concentrations were determined by radioisotopic enzyme assay by the method of Bieber and Lewin. 34 Dogs 1-4 were euthanized and immediate postmortem examinations were performed.…”

Section: Methodsmentioning

confidence: 99%

A Variant Form of Neuronal Ceroid Lipofuscinosis in American Bulldogs

Evans¹,

Katz²,

Levesque³

et al. 2005

J Vet Int Med

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Neuronal ceroid lipofuscinoses (NCLs) are inherited neurodegenerative diseases characterized by accumulations of autofluorescent lipopigments within cells of the nervous system. Nine related American Bulldogs demonstrated dysmetria in all limbs and para-paresis. Nonambulatory tetraparesis was observed only in the later stages of the disease. The clinical signs developed between 1 and 3 years of age and were slowly progressive over several years, which is inconsistent with most reports in other breeds. Results from blood tests for 8 different lysosomal storage diseases on 4 affected and 6 related but unaffected dogs were negative. Four affected dogs were euthanized and histopathologic examinations showed diffuse accumulations of periodic acid-Schiff-positive inclusions in neurons and axonal spheroids along the entire neuraxis and retinae. The most severe lesions were in the brainstem proprioceptive nuclei and spinal cord, consistent with clinical signs. The storage material was autofluorescent and immunohisto-chemically positive for products of lipid peroxidation. Ultrastructural analysis was consistent with NCL. Pedigree analysis supports an autosomal-recessive mode of inheritance. NCL has not been previously reported in the American Bulldog and these findings suggest a variant form of the canine disease.

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Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Cited by 191 publications

References 0 publications

d-Lactic Acidosis Secondary to Exocrine Pancreatic Insufficiency in a Cat

d-Lactic Acidosis Secondary to Exocrine Pancreatic Insufficiency in a Cat

Thiamine Deficiency in a Dog: Clinical, Clinicopathologic, and Magnetic Resonance Imaging Findings

A Variant Form of Neuronal Ceroid Lipofuscinosis in American Bulldogs

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