Quantitative analysis of blood cells and inflammatory factors in wounds

Cerveró-Ferragut, S; López-Riquelme, Natividad; Martín-Tomás, Ester; Massa-Domínguez, B; Pomares-Vicente, J; Soler-Pérez, M; Sánchez-Hernández, José F.

doi:10.12968/jowc.2017.26.3.121

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Vacuum, drainage, or other external devices were used in 124 of the 280 (44%) studies and thus were the most frequently used methods of sample collection 22–27,29–146 ; the vast majority of sample collections using this method (75%, 103/138), were from surgical wounds (Figure 2B). Three other frequently used methods were extraction from absorbent material (31%, 86/280),…”

Section: Resultsmentioning

confidence: 99%

Wound fluid sampling methods for proteomic studies: A scoping review

Harvey

Mellody

Cullum

et al. 2022

Wound Repair Regeneration

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Understanding why some wounds are hard to heal is important for improving care and developing more effective treatments. The method of sample collection used is an integral step in the research process and thus may affect the results obtained. The primary objective of this study was to summarise and map the methods currently used to sample wound fluid for protein profiling and analysis. Eligible studies were those that used a sampling method to collect wound fluid from any human wound for analysis of proteins. A search for eligible studies was performed using MEDLINE, Embase and CINAHL Plus in May 2020. All references were screened for eligibility by one reviewer, followed by discussion and consensus with a second reviewer.Quantitative data were mapped and visualised using appropriate software and summarised via a narrative summary. After screening, 280 studies were included in this review. The most commonly used group of wound fluid collection methods were vacuum, drainage or use of other external devices, with surgical wounds being the most common sample source. Other frequently used collection methods were extraction from absorbent materials, collection beneath an occlusive dressing and direct collection of wound fluid. This scoping review highlights the variety of methods used for wound fluid collection. Many studies had small sample sizes and short sample collection periods; these weaknesses have hampered the discovery and validation of novel biomarkers. Future research should aim to assess the reproducibility and feasibility of sampling and analytical methods for use in larger longitudinal studies.

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Section: Resultsmentioning

confidence: 99%

Wound fluid sampling methods for proteomic studies: A scoping review

Harvey

Mellody

Cullum

et al. 2022

Wound Repair Regeneration

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“…CRP concentration <8 mg/L is considered a normal clinical condition in wounds [ 27 ], whereas higher values can be used to discriminate different critical conditions. Cerveró-Ferragut et al reported that proliferative and inflammatory phases had an average CRP level equal to 90 mg/L, which dropped to 5.2 mg/L in the maturation phase [ 131 ]. An average CRP level of about 66 mg/L was reported for trauma-related chronic wounds [ 132 ], whereas wound infections following surgically managed fractures were associated with a CRP concentration of 141 mg/L [ 133 ].…”

Section: Discussionmentioning

confidence: 99%

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Salvo

Dini

Kirchhain

et al. 2017

Sensors

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Wound assessment is usually performed in hospitals or specialized labs. However, since patients spend most of their time at home, a remote real time wound monitoring would help providing a better care and improving the healing rate. This review describes the advances in sensors and biosensors for monitoring the concentration of C-reactive protein (CRP), temperature and pH in wounds. These three parameters can be used as qualitative biomarkers to assess the wound status and the effectiveness of therapy. CRP biosensors can be classified in: (a) field effect transistors, (b) optical immunosensors based on surface plasmon resonance, total internal reflection, fluorescence and chemiluminescence, (c) electrochemical sensors based on potentiometry, amperometry, and electrochemical impedance, and (d) piezoresistive sensors, such as quartz crystal microbalances and microcantilevers. The last section reports the most recent developments for wearable non-invasive temperature and pH sensors suitable for wound monitoring.

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“…In addition, the proliferation phase includes broplasia and matrix formation, which is extremely important in the formation of granulation tissue. The formation of granulation tissue depends on the broblast, a critical cell in matrix formation [13]. Far from being just a collagen producer, broblasts produce elastin, bronectin, glycosaminoglycan, and proteases, which are responsible for physiological debridement and remodeling [14].…”

Section: Introductionmentioning

confidence: 99%

Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti-inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis

et al. 2022

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Coffea canephora plant stem cells can have bioactive compounds with tissue repairing and antiin ammatory action. This study aimed to develop a liposomal stem cell extract formulation from C. canephora (LSCECC) and to investigate its capacity to contribute to the dynamic mechanisms of tissue repair. The liposome was developed and characterized through the dynamic light scattering technique, atomic force microscopy, and transmission electron microscopy. The excisional full-thickness skin wound model was used and daily topically treated with the LSCECC formulation or vehicle control. On days 2, 7, 14, and 21 after wounding, ve rats from each group were euthanized and the rates of wound closure and re-epithelialization were evaluated using biochemical and histological tests. LSCECC resulted in faster reepithelialization exhibiting a signi cant reduction in wound area of 36.4, 42.4, and 87.5% after 7, 10, and 14 days, respectively, when compared to vehicle control. LSCECC treated wounds exhibited an increase in granular tissue and a proper in ammatory response mediated by the reduction of pro-in ammatory cytokines like TNF-α and IL-6 and an increase of IL-10. Furthermore, wounds treated with LSCECC showed an increase in the deposition and organization of collagen bers at the wound site and improved scar tissue quality due to the increase in transforming growth factor-beta and vascular endothelial growth factor. Altogether, these data revealed that LSCECC may have remarkable applications in skincare cosmetic formulations to support healthy skin. HighlightsA liposomal stem cell extract formulation from C. canephora (LSCECC) was developedThe LSCECC emerged as a potential approach for skincare applications.The LSCECC promotes wound healing resulting in enhanced epithelization.The LSCECC accelerated wound closure and neo-dermal tissue formation.The LSCECC increased the deposition and organization of collagen bers.

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Quantitative analysis of blood cells and inflammatory factors in wounds

Abstract: Our findings suggest that the combination of the markers, percentage of neutrophils and monocytes, platelets, CRP and IgG levels could be useful prognostic indicators of the inflammatory phase.

Cited by 9 publications

References 15 publications

Wound fluid sampling methods for proteomic studies: A scoping review

Wound fluid sampling methods for proteomic studies: A scoping review

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti-inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis

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