Quantitative analysis of cathepsin L mRNA and protein expression during oral cancer progression

Rare amplicons implicate frequent deregulation of cell fate specification pathways in oral squamous cell carcinoma

“…We isolated RNA from consecutive sections of formalin-fixed paraffin-embedded tumor resection or biopsy specimens (Macabeo-Ong et al, 2003) and performed real-time quantitative RT-PCR as described previously (Neve et al, 2002) in the UCSF Comprehensive Cancer Center Genome Analysis Shared Resource Facility. We used ABI Assays-on-Demand Expression assays.…”

Cysteine cathepsins and stefins in head and neck cancer: an update of clinical studies

“…The comparable CB and CL immunohistochemical profiles were described in the study on oral cavity tumors by Vigneswaran et al, 23 whereas conflicting results from some other studies could have resulted from the differences in analytical procedures used (antigens, reagents), low sample numbers in some series, 24,25 and from the inherent biological characteristics of the site of tissue sampling (oral cavity vs. oropharynx vs. other tumor types). 6,7,27 The observed perinuclear cathepsin positivity mainly manifested intracellularly and on the membrane surface outside the tumor cells, was more consistent throughout the studies (Figure 5a).…”

“…6,7,27 The observed perinuclear cathepsin positivity mainly manifested intracellularly and on the membrane surface outside the tumor cells, was more consistent throughout the studies (Figure 5a). [23][24][25][26] Exclusively intracellular immunostaining for stefins reflected the lack of secretory signal sequences on corresponding genes (Figure 5b). 2 Contrary to our observation, in the sections of breast carcinoma tissue and malignant brain tu- mors, SA and/or SB immunoreactivity was described in a minority of cases or was only sporadic.…”

“…[23][24][25][26] So far, to the best of our knowledge, stefins have not been subjected to immunohistochemical evaluation in any of the studies conducted on head and neck carcinomas. The results on spatial distribution of CB and CL immunoreactivity, with perinuclear positivity mainly manifested intracellularly and on the membrane surface outside the tumor cells, reflect their physiological role and are consistent throughout the studies.…”

“…The results on spatial distribution of CB and CL immunoreactivity, with perinuclear positivity mainly manifested intracellularly and on the membrane surface outside the tumor cells, reflect their physiological role and are consistent throughout the studies. [23][24][25][26] …”