Quantitative analysis of cell surface-associated SV40 large t antigen using a newly developed 3H-protein a binding assay

Rinke, Yvonne; Deppert, Wolfgang

doi:10.1016/0042-6822(89)90433-9

Cited by 6 publications

References 20 publications

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Quantitative cytofluorimetric determination of cell membrane‐associated large tumor antigen on SV40‐transformed cells

et al. 1995

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The aim of this study was to quantitate the number of cell membrane-located SV40 large tumor antigen (large T) molecules of SV40-transformed cell lines by cytofluorimetric analysis. Five different SV40-transformed cell lines were labelled by either a biotin-or a fluorescein-conjugated monoclonal antibody, PAb1605, which is specmc for the large T carboxyterminus. The conjugated-antibody fluorescence signals of the stained large T molecules of transformed cells were measured via cytofluorimetry. Comparison of the fluorescence signals of calibrated beads bearing a known number of fluorescein molecules to the signals of conjugated PAb1605 antibodies bound on microbeads to a detbed number of IgG binding sites made it possible to determine the number of antibody-accessible large T molecules per SV40-transformed cell. The numbers ( X lo-*) found per cell were 1.0 (ELONA, hamster), 3.0 (VLM, mouse), 3.5 (mKSA, mouse), 11 (C57SV, mouse), and 5.5 (SVSO, human), respectively. Thus, the technique described allows a precise quantitation of surfaceexposed, antibody-accessible viral antigen expresdoll. 0 1995 WileyLiss, Inc.

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Quantitative cytofluorimetric determination of cell membrane‐associated large tumor antigen on SV40‐transformed cells

et al. 1995

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Regulation and Modulation of the Function of p53

Mukhopadhyay

Maxwell

Roth

1995

P53 Suppressor Gene

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Modification of transplasma membrane oxidoreduction by SV40 transformation of 3T3 cells

Löw

Crane

Grebing

et al. 1991

J Bioenerg Biomembr

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Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change in activity is expressed both by change in Km and Vmax for ferricyanide reduction. The change in activity is not based on surface charge effect or on tight coupling to proton release or on intracellular NADH concentration. With transformation by SV40 virus infection the expression of transferrin receptors increases, which correlates with greater diferric transferrin stimulation of the rate of ferric ammonium citrate reduction in transformed SV40-3T3 cells than in 3T3 cells.

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Quantitative analysis of cell surface-associated SV40 large t antigen using a newly developed 3H-protein a binding assay

Cited by 6 publications

References 20 publications

Quantitative cytofluorimetric determination of cell membrane‐associated large tumor antigen on SV40‐transformed cells

Quantitative cytofluorimetric determination of cell membrane‐associated large tumor antigen on SV40‐transformed cells

Regulation and Modulation of the Function of p53

Modification of transplasma membrane oxidoreduction by SV40 transformation of 3T3 cells

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