Quantitative Analysis of Chemical Components of Hydrolysate from Silkworm Fed with Cudrania tricuspidata Leaves

Abstract

The purpose of this study was to develop hydrolysate from silkworm (Cudrania Silkworm Fibroin Peptide; CSFP), a food containing components for improving blood vessel health. In general, Cudrania tricuspidata leaves contain about 5 times more rutin and 25 times more dihydroquercetin than mulberry leaves. 1-Deoxynojirimycine (1-DNJ), dihydroquercetin and rutin inhibit the activity of carbohydrate-digesting enzymes, inhibit blood lipid peroxidation, and regulate insulin secretion, which helps blood vessels to be healthy. When the diet-controlled silkworm was enzymatically hydrolyzed, it was confirmed that rutin content was about 8 times higher than that of the in general silkworm as a control. In the silkworm hydrolysate, CSFP, developed as a final food material, the active ingredients were 65 mg/kg for rutin, 3,328 mg/ kg for DNJ, 0.43 mg/kg for dihydroquercetin, and 82,624 mg/kg for total polyphenol, which was confirmed through LC-MS/MS analysis. In conclusion, it was found that silkworms fed with C. tricuspidata leaves as a diet had more active components that can help control blood sugar and improve blood vessel health than silkworms fed with mulberry leaves.

Protective Effects on Neuronal SH-SY5Y Cells and Antioxidant Activity of Enzymatic Hydrolyzate from Silkworms Fed the Leaves of Cudrania tricuspidata

“…The polyphenol content of ESLC extracts developed using enzymatic treatment was remarkably higher than that of SLC (p < 0.05). This was similar to the findings of Choi et al [21], who reported that the…”

“…The nutritional and functional components of mulberry leaves significantly affect silkworm growth and quality [19]. Cudrania tricuspidata belongs to the family Moraceae, and compared to ordinary mulberry leaves, the leaves are rich in functional components and bioactive substances such as gamma-aminobutyric acid, rutin, dihydroquercetin, and 1-deoxynojirimycin [20,21]. Particularly, it has been reported to have excellent antioxidant activity and neuroprotective effects owing to its high bioactive substance content, such as polyphenols and flavonoids [22,23].…”

Effects of Hydrolysates from Silkworms Fed Cudrania tricuspidata Leaves on Improvement of Memory in Rats with Impaired Memory Induced by Scopolamine

“…Cudrania tricuspidata (commonly called mandarin melon berry, silkworm thorn, etc.) belongs to the family Moraceae, and its leaves are rich in bioactive substances and possess excellent antioxidant activity compared to Morus alba [30][31][32], especially the content of the neurotransmitter gamma-aminobutyric acid, which has been reported to be effective in improving memory [33]. However, there are no studies on the functional effects of silkworms that are fed Cudrania tricuspidata leaves.…”