Quantitative Analysis of CMV-DNA Load in Renal Transplant Recipients Using Real-Time PCR

Rangbar-Kermani, R; Sharifi, Z; Mahmoodian-Shooshtari, Mahmood; Mousavi, Kobra Zavar

doi:10.21859/isv.5.1.28

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…In this study, higher viral load correlates precisely with the development of CMV-related symptoms and viral loads were slightly lower with asymptomatic patients (high significant difference, p ≤ 0.001), in which 82.4% of patients had clinical symptoms of CMV disease with viral loads > 1000 copies/ml. These observations confirm previous reports by Hadaya and her colleagues [ 32 ], Knipe and Howley [ 33 ], Madi et al [ 24 ], Helanter et al [ 34 ], and Rangbar-Kermani et al [ 35 ]. A medium level of viral load was higher in patients with symptomatic CMV disease than asymptomatic disease.…”

Section: Discussionsupporting

confidence: 92%

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Ahmed

Altayb

Saeed

et al. 2022

BioMed Research International

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Background. Cytomegalovirus (CMV) is the most common opportunistic pathogen among renal transplants with significant morbidity and mortality. This study was designed to detect CMV DNA and to determine the frequency of different glycoprotein B (UL55) genotypes among Sudanese renal transplant recipients. Methods. One hundred and four renal transplant recipients were included in this study. A blood specimen was collected from each recipient. DNA was extracted from plasma using the QIAamp DNA mini kit. CMV amplification and quantification were performed using CMV Real-RT Quant kits. Genotyping of human CMV gB was carried out by nested PCR and sequencing of the highly diverse region of gB. Results. CMV DNA was detected in 40/104 (38.5%) of renal transplant recipients. The average of the CMV DNA viral load was 358 × 10 4 copies/ml (6.5 log10) ranging from 62 copies/ml (1.8 log10) to 1.43 × 10 8 copies/ml (9 log10). CMV viremia was detected in 60% of recipients of less than 1–12 months, 17% of 13–24, 10% of 25–36, 5% of 37–48, and 8% in more than 48 months posttransplantation with no association ( p = 0.296 ) between CMV viremia and postrenal transplantation time. The association between the type of immunosuppressive drugs and high viral loads (>1000 copies/ml) showed a significant difference ( p = 0.05 ). The association between CMV loads of >1000 copies/ml and symptoms of CMV disease was highly significant ( p ≤ 0.001 ). Fever 7 (41%), fever and leucopenia 6 (35%), and gastrointestinal disease 4 (24%) were the most common symptoms of CMV disease. CMV genotyping revealed 8 cases (80%) for gB3 and 2 cases (20%) for gB4 genotypes. The most frequent genotype among Sudanese renal transplant recipients was gB3. Conclusions. The frequency of CMV DNA is high among Sudanese renal transplant recipients. CMV gB3 is the most predominant glycoprotein B genotype in Sudanese renal transplant recipients.

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Section: Discussionsupporting

confidence: 92%

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Ahmed

Altayb

Saeed

et al. 2022

BioMed Research International

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“…In this study higher viral load correlate precisely with the development of CMV-related symptoms and viral loads were slightly lower with asymptomatic patients (high significant difference P. value= 0.00), in which (82.4%) of patients had clinical symptoms of CMV disease with viral loads >1000 copies/ml. This results confirming previous reports by Hadaya her colleges [32], Knipe and Howley [33], Madi et al [24], Helanter et al [34] and Rangbar-Kermani et al [35]. Medium level of viral load was higher in patients with symptomatic CMV disease than asymptomatic.…”

Section: Discussionsupporting

confidence: 91%

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Ahmed

Omer

Altyab

2019

Preprint

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Background Cytomegalovirus (CMV) is the most common opportunistic pathogen among solid organ transplant recipients’ especially renal transplants with significant morbidity and mortality. This study was designed to detect CMV DNA and to determine the frequency of different glycoprotein B (UL55) genotypes among Sudanese renal transplant recipients. Methods One hundred and four renal transplant recipients were included in this study. A blood specimen was collected from each recipient. DNA was extracted from plasma using QIAamp DNA mini kit. CMV amplification and quantification (estimation of viral load) was performed using CMV Real – RT Quant kits. Genotyping of Human CMV gB was carried out by nested PCR and sequencing of the highly diverse region of glycoprotein B. Results Cytomegalovirus (CMV) DNA (viremia) was detected in 40/104 (38.5%) of renal transplant recipients. The average of CMV DNA viral load was 358 x104 copies/ml (6.5 log10) ranged from 62 copies/ml (1.8 log 10) to 1.43x108 copies/ml (9 log10). CMV viremia was detected in (60%) of recipients of less than 1-12 months, (17%) of 13-24, (10%) of 25-36, (5%) of 37- 48 and (8%) in more than 48 months post-transplantation with no significant association (P. value = 0.296) between CMV viremia and post renal transplantation time. The association between the type of immunosuppressive drugs and high viral loads (more than 1000 copies /ml) showed a significant difference (P. value =0.05). The correlation between CMV loads of more than 1000 copies/ml and the presence of symptoms of CMV disease were highly significant (P.value =0.00). Fever 7(41%), fever and leucopenia 6(35%) and gastrointestinal disease 4(24%) were the most common presenting symptoms of CMV disease. CMV-genotyping revealed 8 cases (80%) for gB3, and 2 cases (20%) for gB4 genotypes. The most frequent genotype among Sudanese renal transplant recipients was gB3 and no mixed genotypes were observed. Conclusions The frequency of CMV DNA is high among Sudanese renal transplant recipients. CMV viremia viral loads were slightly lower in asymptomatic patients. CMV gB3 is the most predominant glycoprotein B genotype in Sudanese renal transplant recipients.

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“…With current preventive strategies and the use of ganciclovir and valganciclovir, the incidence of HCMV is about 17-37% in the first 100 days after transplantation [11] . Quantitative detection of HCMV DNA is a valuable diagnostic tool for detecting HCMV in the early stages of infection before disease occurs [12,13] . Objectives: The purpose of this study is to evaluate the viral load of active HCMV infection in the plasma samples of individuals suspected of kidney transplant rejection and to investigate host and risk factors associated with the activation of this virus in these patients.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Human Cytomegalovirus Viral Load in Kidney Transplant Recipients in Tehran, Iran

Eslami Kojidi,

Shatizadeh Malekshahi,

Jabbari

2023

IEM

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Background:The purpose of this study is to evaluate the viral load of active human cytomegalovirus (HCMV) infection in the plasma samples of people suspected of kidney transplant rejection and to investigate the host and risk factors related to the activation of HCMV in these patients. Materials & Methods: This cross-sectional study was conducted between December 2022 and June 2023. In this study, 98 blood samples related to patients suspected of kidney transplant rejection referring to Labbafinezhad hospital in Tehran were collected. The samples were tested by the GeneProof Cytomegalovirus (CMV) PCR Kit to determine HCMV viral load. ROC curve analysis was used to determine the viral load cutoff point. Findings: HCMV viremia was detected in 18 (18.36%) of 98 transplant recipients. The median viral load in the HCMV viremia group was 24914.0 IU/ml (5147.0-155106.5). The optimal cut-off value for HCMV was determined 778 IU/ml using ROC analysis. Duration of time after transplantation in the viremia and no viremia groups was 120.5 and 46 months, respectively with a statistically significant difference (P=0.014). Conclusion:This study provides valuable insights into the prevalence of HCMV viremia and its associated risk factors in kidney transplant recipients suspected of rejection. The study also highlights the importance of post-transplant monitoring and preventive measures to address viral infections. . Long-term studies with larger sample sizes are needed to evaluate the role of factors influencing the occurrence of viremia after transplantation.

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Quantitative Analysis of CMV-DNA Load in Renal Transplant Recipients Using Real-Time PCR

Cited by 3 publications

References 2 publications

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients

Assessment of Human Cytomegalovirus Viral Load in Kidney Transplant Recipients in Tehran, Iran

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