Quantitative Analysis of Collective Migration by Single-Cell Tracking Aimed at Understanding Cancer Metastasis

Xin, Zhuohan; Deguchi, Keiko; Suye, Shin‐ichiro; Fujita, Shin

doi:10.3390/ijms232012372

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Several biological assays, such as migration, proliferation, and cell-cycle assays, are based on single-cell time-lapse microscopy [14][15][16][17]. The reliability of such assays largely depends on the assay's ability to properly track each single cell over a stack of images.…”

Section: Introductionmentioning

confidence: 99%

Automated identification and tracking of cells in Cytometry of Reaction Rate Constant (CRRC)

et al. 2023

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Cytometry of Reaction Rate Constant (CRRC) is a method for studying cell-population heterogeneity using time-lapse fluorescence microscopy, which allows one to follow reaction kinetics in individual cells. The current and only CRRC workflow utilizes a single fluorescence image to manually identify cell contours which are then used to determine fluorescence intensity of individual cells in the entire time-stack of images. This workflow is only reliable if cells maintain their positions during the time-lapse measurements. If the cells move, the original cell contours become unsuitable for evaluating intracellular fluorescence and the CRRC experiment will be inaccurate. The requirement of invariant cell positions during a prolonged imaging is impossible to satisfy for motile cells. Here we report a CRRC workflow developed to be applicable to motile cells. The new workflow combines fluorescence microscopy with transmitted-light microscopy and utilizes a new automated tool for cell identification and tracking. A transmitted-light image is taken right before every fluorescence image to determine cell contours, and cell contours are tracked through the time-stack of transmitted-light images to account for cell movement. Each unique contour is used to determine fluorescence intensity of cells in the associated fluorescence image. Next, time dependencies of the intracellular fluorescence intensities are used to determine each cell’s rate constant and construct a kinetic histogram “number of cells vs rate constant.” The new workflow’s robustness to cell movement was confirmed experimentally by conducting a CRRC study of cross-membrane transport in motile cells. The new workflow makes CRRC applicable to a wide range of cell types and eliminates the influence of cell motility on the accuracy of results. Additionally, the workflow could potentially monitor kinetics of varying biological processes at the single-cell level for sizable cell populations. Although our workflow was designed ad hoc for CRRC, this cell-segmentation/cell-tracking strategy also represents an entry-level, user-friendly option for a variety of biological assays (i.e., migration, proliferation assays, etc.). Importantly, no prior knowledge of informatics (i.e., training a model for deep learning) is required.

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Section: Introductionmentioning

confidence: 99%

Automated identification and tracking of cells in Cytometry of Reaction Rate Constant (CRRC)

et al. 2023

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“…Pathological observations show that malignant cancer cells frequently invade the surrounding normal tissue in collective rather than individual cell migration. 1 , 2 For individual cell migration, it has been found that the Rho/ROCK signaling is up-regulated and correlates with disease progression. 3 Meanwhile, Rho activates myosin-II and the actomyosin-mediated contraction creates tension within the cells.…”

mentioning

confidence: 99%

Myosin regulates intracellular force and guides collective cancer cell migration via the FAK-Rho/ROCK feedback loop

Qin¹,

He²,

Zhang³

et al. 2023

Genes & Diseases

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Quantitative Analysis of Collective Migration by Single-Cell Tracking Aimed at Understanding Cancer Metastasis

Cited by 2 publications

References 27 publications

Automated identification and tracking of cells in Cytometry of Reaction Rate Constant (CRRC)

Automated identification and tracking of cells in Cytometry of Reaction Rate Constant (CRRC)

Myosin regulates intracellular force and guides collective cancer cell migration via the FAK-Rho/ROCK feedback loop

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