Quantitative analysis of disaturated lecithins in human plasma by ammonia chemical ionization mass spectrometry

Yergey, Alfred L.; Kody, Michael H.; Gershfeld, Norman L.

doi:10.1002/bms.1200081008

Cited by 6 publications

(1 citation statement)

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“…In a previous study the presence of MH+ -32 and MH+ -89 ions was reported in the CI(NH3) spectrum of a 1-0-alkyllysophosphatidylcholine. 22 It was proposed that these rearrangement ions arise from the loss of methanol and 7V,A-dimethylethanolamine, possibly by the transposition of one of the VV-methyl groups to the phosphate group prior to fragmentation (Figure 3). We have observed similar ions, together with a loss of one of the fatty acyl groups (ions e and g).…”

Section: Methodsmentioning

confidence: 99%

Mass spectrometry of phospholipids. Some applications of desorption chemical ionization and fast atom bombardment

Ayanoğlu¹,

Wegmann²,

PILET³

et al. 1984

J. Am. Chem. Soc.

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53) Lemieux, R. U.; Bock, K.; Delbaere, L. T. J.; Koto, S.; Rao, V. S. (54) Maggio, B.; Cumar F. A,; Caputto, R.Abstract: Major diagnostic peaks in desorption chemical ionization (D/CI) and fast atom bombardment (FAB) mass spectra of various model synthetic phospholipids and related compounds are reported in conjunction with our ongoing research on marine phospholipids. Similarities and differences with some previous studies are presented. Commercially available or partially synthesized, saturated or unsaturated fatty acid containing phospholipids with different head groups such as choline, ethanolamine, mono-and dimethylethanolamine, serine, and glycerol were investigated. For identification purposes, 1,2-diacetylglycerol, 1,2-diacetyl-sn-glycero-3-phosphocholine, and 1 ,2-dipalmitoyl-sn-glycero-3-phosphocholine-d9 were also prepared and their mass spectral behavior studied. In terms of diagnostically useful fragmentations ammonia proved to be superior to methane as reagent gas for chemical ionization. The use of deuterated ammonia shed light on the nature of several fragmentations. In most cases, both chemical ionization and fast atom bombardment techniques exhibited molecular ions and/or related peaks. Desorption chemical ionization with ammonia provided more information about fatty acyl moieties, while fast atom bombardment gave diagnostic peaks about various head groups. These two techniques thus offer complementary information about the structures of intact phospholipids.Phospholipids, together with sterols, are among the main classes While electron-impact mass spectrometry has proved to be a very powerful technique in the structure elucidation of sterols, difficulties of lipids in the cell membranes of living organisms.

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Section: Methodsmentioning

confidence: 99%