Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography

Crozier, Alan; Jensen, Einar; Lean, Michael E. J.; McDonald, Morag S.

doi:10.1016/s0021-9673(96)00826-6

Cited by 150 publications

(125 citation statements)

References 17 publications

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“…Flavonoids are polyphenolic compounds, which are widely found in plant kingdom. As intrinsic components of fruits, vegetables, and beverages many of the 4000 different flavonoids known to-date, are present in a common regular diet [65]. Pigeonpea leaves also contain other compounds such as hordenine, juliflorine, betulinic acid, stigmasterol, beta-sitosterol, etc.…”

Section: Chemical Constituents Of Leavesmentioning

confidence: 99%

Quality nutrition through pigeonpea—a review

Saxena¹,

Kumar²,

Sultana³

2010

Health

136

109

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Protein mal-nutrition is widespread among poor of developing and under developed countries. Since animal protein is beyond the reach of this group, their primary protein supply comes from plant based products. Amongst these, pigeonpea or red gram (Cajanus cajan (L.) Millspaugh) is an important food legume that can be grown under rainfed conditions with least inputs. Pigeonpea is rich in starch, protein, calcium, manganese, crude fiber, fat, trace elements, and minerals. Besides its high nutritional value, pigeonpea is also used as traditional folk medicine in India, China, Philippines and some other nations. Literature on this aspect show that pigeonpea is capable to prevent and cure a number of human ailments such as bronchitis, coughs, pneumonia, respiratory infections, dysentery, menstrual disorders, sores, wounds, abdominal tumors, tooth ache, and diabetes.

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Section: Chemical Constituents Of Leavesmentioning

confidence: 99%

Quality nutrition through pigeonpea—a review

Saxena¹,

Kumar²,

Sultana³

2010

Health

136

109

View full text Add to dashboard Cite

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“…For analysis of the flavonoid content, the extraction and hydrolysis conditions were based on the method described by Crozier et al (1997). The oven-dried calli were extracted with 10 mL of 60% aqueous methanol containing 20 mM sodium diethyldithiocarbamate (NaEDTC) as an antioxidant.…”

Section: Analysis Of Flavonoid Contentmentioning

confidence: 99%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L −1 sucrose supplemented with different concentrations (0.5-2.5 mg L ). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture

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“…These maxima correspond to bands I and II, typical in this class of compounds. Analysis of the 1 H and 13 C NMR spectra of both compounds and comparison with literature data 8,9 allowed us to characterize them as eupafolin (3) and hispidulin (4).…”

Section: Resultsmentioning

confidence: 99%

“…In another study 3 it was used for the quantitative analysis of flavonoid aglycones. A comparison of reversed-phase columns has also been made 4 showing considerable discrepancy in performance.…”

Section: Introductionmentioning

confidence: 99%

HPLC analysis of flavonoids in Eupatorium littorale

Oliveira¹,

Nakashima²,

Filho³

et al. 2001

J. Braz. Chem. Soc.

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Neste trabalho são descritos o isolamento e a identificação de eupafolina e hispidulina das folhas secas de Eupatorium littorale (Asteraceae). Foi desenvolvido também um método para a detecção desses flavonóides e de rutina na planta, utilizando a técnica de CLAE em fase reversa com detetor de arranjo de fotodiodos. Eupafolina e hispidulina foram quantificados sendo encontradas as concentrações de 0,29% e 0,21%, respectivamente.The isolation and identification of eupafolin and hispidulin in the dried leaves of Eupatorium littorale (Asteraceae) are described. A method for the detection of those flavonoids and rutin was also developed using reversed-phase HPLC with a photodiode array detector. Eupafolin and hispidulin were quantified and the concentrations found were 0.29% and 0.21%, respectively.

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Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography

Cited by 150 publications

References 17 publications

Quality nutrition through pigeonpea—a review

Quality nutrition through pigeonpea—a review

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

HPLC analysis of flavonoids in Eupatorium littorale

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