Quantitative analysis of food fatty acids by capillary gas chromatography

Slover, Hal T.; Lanza, Elaine

doi:10.1007/bf02674138

Cited by 391 publications

(162 citation statements)

References 11 publications

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“…Peaks were identified by comparison of their retention times with those of authentic reference compounds (Sigma-Aldrich, St. Louis, MO, USA). Fatty acid metal esters were determined by GC according to the method described by Slover and Lanza (1979) with minor modifications. Fatty acid metil esters were prepared using boron trifluoride in methanol (20% of BF 3 in methanol) and extracted with n-hexane and then analyzed by GC.…”

Section: Methodsmentioning

confidence: 99%

Chemical and nutritional changes in bitter and sweet lupin seeds (Lupinus albus L.) during bulgur production

Yorgancılar

Bilgiçli

2012

J Food Sci Technol

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In this research, bitter and sweet Lupin (Lupinus albus L.) seeds were used in bulgur production. The proximate chemical compositions and the contents of phytic acid, mineral, amino acid and fatty acid of raw material and processed lupin seeds as bulgur were determined. The sensory properties of bulgur samples were also researched. Bulgur process decreased ash, fat and phytic acid content of lupin seeds while significant increase (p<0.05) was observed in protein content of bulgur compared with lupin seeds. Phytic acid losses in bitter and sweet lupin bulgurs were found as 18.8% and 21.3%, respectively. Generally sweet lupin seeds/bulgurs showed rich essential amino acids composition than that of bitter seeds/bulgurs. Linoleic and linolenic acid content of the lupin was negatively affected by bulgur process. Bitter lupin bulgur received lower scores in terms of taste, odor and overall acceptability than sweet lupin bulgur in sensory evaluation. Sweet lupin bulgur can be used as new legume-based product with high nutritional and sensorial properties.

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Section: Methodsmentioning

confidence: 99%

Chemical and nutritional changes in bitter and sweet lupin seeds (Lupinus albus L.) during bulgur production

Yorgancılar

Bilgiçli

2012

J Food Sci Technol

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“…were prepared in the presence of boron trifluoride by means of the method of Slover and Lanza [23]. Methyl esters were extracted with hexane, then separated by gas/liquid chromatography.…”

Section: Gadliquid-chromatography Analysis Fatty Methyl Estersmentioning

confidence: 99%

Localization and Regulation of the Putative Membrane Fatty‐Acid Transporter (FAT) in the Small Intestine

Poirier

Degrace

Niot

et al. 1996

European Journal of Biochemistry

198

131

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The expression of the putative membrane fatty-acid transporter (FAT) was investigated in the small intestine. The FAT mRNA level was higher in the jejunum than in the duodenum and was lower in the ileum, as observed for cytosolic fatty-acid-binding proteins (FABP) expressed in this tissue. No FAT transcript was found in the stomach or colon. FAT mRNA was constitutively expressed i n the epithelial cells located in the upper two thirds of villi, while it was undectectable in the crypt cells and submucosal cells. In jejunal mucosa, immunochemical studies showed that FAT protein was limited to the brush border of enterocytes. No fluorescence was found in the goblet cells. To determine whether FAT responded to changes in fat intake, as reported for FABP, the effect of two high-fat diets, which essentially contained either medium-chain fatty acids or long-chain fatty acids (sunflower-oil diet), was investigated. The sunflower-oil diet greatly increased FAT mRNA abundance throughout the small intestine. In contrast, a weak effect of medium-chain fatty acids was observed only in the jejunum. As found for FABP expression, treatment with the hypolipidemic drug bezafibrate affected FAT expression. These data demonstrate that FAT and FABP are co-expressed in enterocytes, as has been shown in adipocytes, myocytes and mammary cells. The data suggest that these membrane and cytosolic proteins might have complementary functions during dietary-fat absorption.Keywords: fatty-acid transporter ; fatty-acid-binding protein ; small intestine ; gene regulation ; lipid.How long-chain fatty acids (LCFA) move across the biological membranes is subject to controversy. Because of their lipophilic character, they were expected to diffuse freely through the plasma membrane of cells [I]. However, this concept was challenged by the finding of a rapid and saturable uptake, which was reduced by prior heat denaturation or protease treatment of cells 121, and by the isolation and characterization of several plasma-membrane proteins that showed high affinity for LCFA [2-51. The small intestine might express at least two of these proteins: the plasma-membrane fatty-acid-binding protein (FABP) and the fatty-acid transporter (FAT) [6]. The plasmamembrane FABP is a 40-kDa protein postulated to mediate fatty acid uptake through an active sodium-dependent process in the gut 17, 81. The regulation of this protein is unknown since it has not been cloned. FAT is a 88-kDa membrane protein, which was cloned recently in adipocytes, where it might be involved in the sequestration and uptake of fatty acids 191. This protein, which is similar to the CD36 glycoprotein [lo], was detected by means of Northern blot analysis in various tissues including the small intestine [9].Once within the cell, LCFA bind to 14-1 5-kDa cytosolic proteins termed fatty-acid-binding proteins (FABP), which are thought to be involved in facilitation of LCFA desorption from the plasma membrane and diffusion of LCFA through the cytosol [ll]. Several recent reports showed that the express...

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“…Total fat (±10 mg) was methylated to prepare fatty acid methyl esters (FAME) for gas chromatographic (GC) analysis by using methanol-BF3 (Slover and Lanza, 1979). The FAME were quantified using a Varian GX 3400 flame ionization GC, with a fused silica capillary column, Chrompack CPSIL 88 (100 m length, 0.25 μm ID, 0.2 μm film thickness).…”

Section: Methodsmentioning

confidence: 99%

Comparative biochemistry and physiology

1978

Comparative Biochemistry and Physiology Part A: Physiology

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Milk was obtained from two captive bred cheetahs. The nutrient content was 99.6 g protein; 64.8 g fat; and 40.21 g lactose per kg milk. Small amounts of oligosaccharides, glucose, galactose and fucose were noted. The protein fraction respectively consisted of 34.2 g caseins per kg milk and of 65.3 g whey proteins per kg milk. Very little variation in milk composition among the individual cheetahs was noted. Electrophoresis and identification of protein bands showed a similar migrating sequence of proteins as seen in lion's and cat's milk, with small differences in the â-caseins. The lipid fraction contains 290.4 g saturated and 337.3 g mono-unsaturated fatty acids per kg milk fat respectively. The high content of 279.5 g kg-1 milk fat of polyunsaturated fatty acids is due to a high content in á-linolenic acid. No short chain fatty acids, but substantial levels of uneven carbon chain fatty acids were observed. AbstractMilk was obtained from two captive bred cheetahs. The nutrient content was 99.6 g protein; 64.8 g fat; and 40.21 g lactose per kg milk. Small amounts of oligosaccharides, glucose, galactose and fucose were noted. The protein fraction respectively consisted of 34.2 g caseins per kg milk and of 65.3 g whey proteins per kg milk. Very little variation in milk composition among the individual cheetahs was noted. Electrophoresis and identification of protein bands showed a similar migrating sequence of proteins as seen in lion's and cat's milk, with small differences in the β-caseins. The lipid fraction contains 290.4 g saturated and 337.3 g mono-unsaturated fatty acids per kg milk fat respectively. The high content of 279.5 g kg − 1 milk fat of polyunsaturated fatty acids is due to a high content in α-linolenic acid. No short chain fatty acids, but substantial levels of uneven carbon chain fatty acids were observed.

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Quantitative analysis of food fatty acids by capillary gas chromatography

Cited by 391 publications

References 11 publications

Chemical and nutritional changes in bitter and sweet lupin seeds (Lupinus albus L.) during bulgur production

Chemical and nutritional changes in bitter and sweet lupin seeds (Lupinus albus L.) during bulgur production

Localization and Regulation of the Putative Membrane Fatty‐Acid Transporter (FAT) in the Small Intestine

Comparative biochemistry and physiology

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