Quantitative Analysis of Induced Phenotypic Diversity in Chickpea Using Physical and Chemical Mutagenesis

Abstract

A B S T R A C TIn the present scenario of variable natural environment and sky-high population, sustainable boost in the agricultural productivity is the utmost priority. Induce mutagenesis generates noble genetic combination without affecting the overall genomic makeup of crop, thus, providing essential genetic variation for any crop improvement programme. The present study has been carried out to investigate the comparative mutagenicity of gamma rays and HZ on chickpea (Cicer arietinum L.) genotype (avrodhi) at M 2 generation developed from seeds of treated M 1 plants population. The assessment on phenotypic expression for the studied qualitative and quantitative traits showed considerable deviations in all the treatments and significant positive shift in 0.01 and 0.02% doses compared to control while 0.04% proved to be most mutagenic with highest significant negative deviation. A broad spectrum and frequency of macro mutations were induced affecting all plant parts and different morphological variants were screened and isolated on the basis of economic importance from the treated populations. Economically important mutations like branching pattern, stem structure, plant height, dwarf and bushy growth habit, foliage type, flowering behavior and maturity were identified and the frequency of the variants were found to be more in 0.03% doses. Explicitly, HZ doses provided greater deviations in both directions in the quantitative phenotypic characters studied while frequencies of distinct morphological mutants were more in gamma rays. The induced elite phenotypes (blue flowered, double flowered, pigmented leaf, bushy and early mutants), having strong correlation with agronomic traits, will definitely be helpful in selection of improved mutants in subsequent generations.

Induce Physical and Chemical Mutagenesis for Improvement of Yield Attributing Traits and their Correlation Analysis in Chickpea

“…This assessment also assists to understand the mean deviation induced in the agronomic traits. In chickpea, several morphological mutants based on growth habit, size, leaf, flower and seeds were reported [4,9,[14][15][16][17] with the application of single and combination treatments of physical and chemical mutagens. Since, every individual gene responsible for trait of agronomic interest can mutate, therefore, a wide spectrum of viable morphological mutants can be expected in mutation experiments [18].…”

“…The improvement of economic traits and quality characters for improvement of crop within the short period of time can be achieved by the help of induced mutagenesis [5,6]. Induced morphological mutants are useful for the development of improved varieties when they are used in cross breeding programmes [4,7,8]. Chickpea (Cicer arietinum L.) is an important nutritional pulse crop.…”

Assessment on induced genetic variability and divergence in the mutagenized lentil populations of microsperma and macrosperma cultivars developed using physical and chemical mutagenesis

“…[ 7 ] reported that the lentil germplasm from India exhibits least genetic variability among lentil-producing countries. The adaptation to various stresses through natural selection for high productivity, seed quality, pest and disease resistance and inefficiency of conventional breeding methods exhausted the genetic variability of grain legumes[ 8 ]over the course of time, which resulted in limited accessible genetic variability and hence supplemented breeding strategies needs to be incorporated to serve the objective of crop improvement[ 9 ].Induced mutations technology, over the past decades, appeared to be one of the most coherent approaches for broadening the genetic base in lentil to circumvent the bottleneck conditions. Also, it is highly recommended especially in lentil due to its tiny flower size which makes recombination breeding a very tedious job.…”

Identification of induced mutations in hexaploid wheat genome using exome capture assay

“…In chickpea, the effectiveness and efficiency of EMS were seven and two times higher than that of γ rays [ 53 ]. In another study, effectiveness of hydrazine hydrates (HZ) towards inducing mutations in quantitative traits was higher than by the γ rays but less than the EMS [ 54 ]. However, in another study, similar effectiveness by exposing with of both the mutagens were found on Stevia rebaudiana [ 55 ].…”