Quantitative Analysis of Interleukin‐1‐Alpha Gene Expression in Middle Ear Cholesteatoma

Bujía, J; Kim, C.; Boyle, D.; Hammer, C.; Firestein, Gary S.; Kastenbauer, E.

doi:10.1097/00005537-199602000-00021

Cited by 23 publications

(27 citation statements)

References 16 publications

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“…Several previous studies examined the role of inflammatory cytokines in the pathogenesis of cholesteatoma, including the osteoclastogenic tumor necrosis factor-alpha (TNF-␣; 6,8,19,22,23). Serum levels of TNF-␣ were higher in patients who had more than 2 ossicles destroyed (22).…”

Section: Discussionmentioning

confidence: 99%

Expression of Matrix-Degrading Cysteine Proteinase Cathepsin K in Cholesteatoma

et al. 2001

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Cholesteatoma is a nonneoplastic lesion of the middle ear space or mastoid that is histologically characterized by a progressive bone erosion of the ossicles and surrounding bone. Several matrixdegrading enzymes have been implicated as mediators of this bone erosion. Because the novel cysteine proteinase cathepsin K has been shown to play a central role in bone resorption, we examined the expression of this enzyme in tissue specimens of cholesteatoma. Tissue specimens of 9 patients with cholesteatoma were obtained during middle-ear surgery. Expression of cathepsin K mRNA was determined by RT-PCR using specific primers. Immunohistochemical analysis of cathepsin K protein expression in tissue sections was performed by using the streptavidin-alkaline phosphatase technique. Expression of both cathepsin K mRNA and protein was detected in areas affected by cholesteatoma, whereas specimens of nonaffected ear cartilage and surrounding tissue were not positive. In addition, cathepsin K was detected in numerous multinucleated giant cells, particularly osteoclasts at the site of bone degradation. In contrast, keratinized squamous epithelium was negative for cathepsin K. These data demonstrate that the matrix-degrading cysteine proteinase cathepsin K may be involved in bone erosion in cholesteatoma. Strong expression of this collagenolytic enzyme in osteoclasts suggests that these cells are mainly involved in cathepsin K-mediated bone destruction.

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Section: Discussionmentioning

confidence: 99%

Expression of Matrix-Degrading Cysteine Proteinase Cathepsin K in Cholesteatoma

et al. 2001

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“…A number of studies [15,16] have attempted to delineate a Th1/Th2 paradigm of inflammatory events in cholesteatomas, in which Th1 cells characterized by the production of interferon-␥ , TNF-␣ and IL-1 are likely to be dominant. A third T cell subset known as Th17 was identified and characterized by the production of IL-17 [17,18] .…”

Section: Discussionmentioning

confidence: 99%

Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma

Haruyama

Furukawa²,

Kusunoki³

et al. 2010

ORL

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Purpose: The present study was designed to elucidate the immunoreactivity and protein level of IL-17 in human cholesteatomas. Procedures: The expression and localization of IL-17 and receptor activator of nuclear factor ĸB ligand (RANKL) were examined by immunohistochemistry in tissue specimens collected from 24 patients with cholesteatomas. The cellular sources of IL-17 were assessed by double staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivity. Results: IL-17 immunoreactivity was detected in the inflammatory cells in the granulation tissues and there were increased levels of IL-17 protein. The localization of IL-17 expression coincided with CD4-positive lymphocytes. IL-17 was correlated with the cells positive for RANKL. The degree of bone destruction was dependent on the number of infiltrated IL-17-positive cells. IL-17-driven pathology was observed in the congenital type as compared with the acquired type. Conclusions: The present study suggests that IL-17 is related to the pathogenesis of the disease.

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“…Marenda & Aufdemorte 8 and Bujia et al 10 described Interleukin 1 (IL1) in acquired cholesteatoma. They agreed in their findings reporting IL 1 immunoexpression in all layers of the epithelium and subepithelium of the cholesteatoma.…”

Section: Discussionmentioning

confidence: 99%

“…High concentrations of IL-6 were related to erosion of ossicular chain and presence of intra-operative granulation tissue. IL-8 promotes the production of collagenases, which are proteins involved in process of bone lysis 8,10,11 . In reference to immune localization Tumor Necrosis Factor-Alpha (TNF-α) in acquired cholesteatoma, the authors disagreed.…”

Section: Discussionmentioning

confidence: 99%

“…It is synthesized under two forms: IL-1α and IL-1β 7 . Several authors described IL1 in acquired cholesteatoma [8][9][10][11][12][13] . Interleukin 6 (IL-6) is a cytokine of 26 kD, synthesized by many different types of macrophages (primarily), lymphocyte T and B, fibroblasts, cells of bone marrow stroma and epithelial and endothelial cells.…”

Section: Cholesteatoma Cytokinesmentioning

confidence: 99%

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O papel das citocinas no colesteatoma adquirido da orelha média: revisão da literatura

Alves¹,

Ribeiro²

2004

Rev. Bras. Otorrinolaringol.

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Este estudo visa a analisar de modo crítico a literatura pertinente a respeito do papel das citocinas no colesteatoma adquirido. O colesteatoma da orelha média é caracterizado pela presença de epitélio escamoso estratificado queratinizado neste local, com alto poder invasivo, causando destruição óssea e podendo levar a complicações. As citocinas são glicoproteínas de baixo peso molecular que atuam na intercomunicação celular. São importantes na estimulação e supressão dos eventos da resposta imune, desencadeando e coordenando a resposta inflamatória, assim como os processos de cicatrização e remodelação tecidual. No colesteatoma já foram observadas as seguintes citocinas e fatores de crescimento: IL-1, IL-6, IL-8, TNF-±, TGF- ±,TGF-², EGF e KGF. Ocorre um sinergismo entre as diferentes citocinas para resultar nas características agressivas do colesteatoma.

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Quantitative Analysis of Interleukin‐1‐Alpha Gene Expression in Middle Ear Cholesteatoma

Cited by 23 publications

References 16 publications

Expression of Matrix-Degrading Cysteine Proteinase Cathepsin K in Cholesteatoma

Expression of Matrix-Degrading Cysteine Proteinase Cathepsin K in Cholesteatoma

Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma

O papel das citocinas no colesteatoma adquirido da orelha média: revisão da literatura

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