Quantitative analysis of intracellular coenzymes in Saccharomyces cerevisiae using ion pair reversed phase ultra high performance liquid chromatography tandem mass spectrometry

Seifar, Reza M.; Ras, Cor; Deshmukh, Amit T.; Bekers, Katelijne M.; Suarez-Mendez, Camilo A.; Cruz, Ana L.B. da; Gulik, Walter M. van; Heijnen, Joseph J.

doi:10.1016/j.chroma.2013.08.076

Cited by 55 publications

(48 citation statements)

References 28 publications

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“…Several methods are described to stop metabolism, such as rapid cooling, especially quick freezing in liquid nitrogen for tissues, and mixing the cultured cells with organic solvents [29] (in many cases with cold organic solvent) [5] are often used. Less common methods, but still with importance, are the heat treatment of the system [31,32], and acidification. But while considering the use of these techniques, it must be ensured that all the analytes to be examined are heat or acid stable.…”

Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

“…Preheated at 95 °C, 75% ethanol was used for extraction followed by drying -resuspension -centrifugation to obtain nucleotides from yeast culture [31]. In another case, precooled, aqueous 60% (v/v) methanol buffered with 10 mM ammonium acetate (pH 7.5) was used as an extraction solvent [32].…”

Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

“…When using IP reagents, the stationary phase is a usual reversed phase column. Ion-pair reagents are normally alkylamines, such as dibutylammonium acetate (DBAA) [32], tributylamine (TBA) [5-7, 29, 31], dimethylhexylamine (DMHA) [8,34], dibutylamine (DBA) [4], hexylamine (HA) [7] for negative ionization mode. As nucleotides are detected in negative mode, ion suppression is less problematic.…”

Section: Liquid Chromatographymentioning

confidence: 99%

“…cer.) [9,31,32], and Methylobacterium extorquens AM1 [33] was monitored, and whole blood [2,12], serum and plasma [6,7,34] urine [6], cultured cells [6-9, 30, 34, 35], cerebrospinal fluid [34], and tissues [4,6,7,31,34] were used as samples.…”

Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

See 3 more Smart Citations

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

Somogyi

Horvai

Csala

et al. 2016

Period. Polytech. Chem. Eng.

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Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

Section: Liquid Chromatographymentioning

confidence: 99%

Section: Analysis Of Endogenous Pyridine Dinucleotidesmentioning

confidence: 99%

See 2 more Smart Citations

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

Somogyi

Horvai

Csala

et al. 2016

Period. Polytech. Chem. Eng.

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“…Single analyte standard dissolved in 50% acetonitrile solution was infused at a flow rate of 5 lL/min for tuning compound-dependent MS parameters. The major MS/MS fragment patterns of each analyte and its 13 C derivative were adopted from the study of Seifar et al [21,22] and were listed in Table 1. Tube lens and collision energy (CE) of each transition were optimized.…”

Section: Determination Of Intracellular Metabolites Via Lc-ms/msmentioning

confidence: 99%

13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea

Hong

Mou

Liu

et al. 2017

Bioprocess Biosyst Eng

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Metabolomics analysis is extremely essential to explore the metabolism characteristics of Saccharopolyspora erythraea. The lack of suitable methods for the determination of intracellular metabolites, however, hinders the application of metabolomics analysis for S. erythraea. Acyl-CoAs are important precursors of erythromycin; phosphorylated sugars are intermediate metabolites in EMP pathway or PPP pathway; organic acids are intermediate metabolites in TCA cycle. Reliable determination methods for intracellular acyl-CoAs, phosphorylated sugars, and organic acids of S. erythraea were designed and validated in this study. Using the optimized determination methods, the pool sizes of intracellular metabolites during an erythromycin fermentation process were precisely quantified by isotope dilution mass spectroscopy method. The quantification results showed that the specific erythromycin production rate was positively correlated with the pool sizes of propionyl-CoA as well as many other intracellular metabolites. The experiment under the condition without propanol, which is a precursor of propionyl-CoA and an important substrate in industrial erythromycin production process, also corroborated the correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size. As far as we know, this is the first paper to conduct the metabolomics analysis of S. erythraea, which makes the metabolomics analysis of S. erythraea in the industrial erythromycin production process possible.

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Flow injection assays for NADH and ethanol using photosensitized rose bengal and luminol–copper (II) chemiluminescence system

2023

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The online photoreaction of the rose bengal photosensitized luminol-copper (II) chemiluminescence (CL) system was used for the determination of β-nicotinamide adenine dinucleotide (NADH) and ethanol (EtOH) in pharmaceutical formulations combined with a flow injection technique. NADH can significantly enhance the CL emission of the reaction. For EtOH, alcohol dehydrogenase in soluble form was utilized in the presence of nicotinamide adenine dinucleotide resulting in NADH production. The limit of detection (3σ blank, n = 3) of 4.0 Â 10 À8 and 2.17 Â 10 À5 M, and linear range 1.3 Â 10 À7 to 2.5 Â 10 À5 M (R 2 = 0.9998, n = 6) and 0.11-2.17 Â 10 À3 M (R 2 = 0.9996, n = 6) were obtained for NADH and EtOH respectively. The injection rate was 100 h À1 with a relative standard deviation (n = 3) of 1.5-4.8% in the range studied for both analytes. The procedure was satisfactorily applied to pharmaceutical formulations with recoveries in the range 91.6 ± 3.0% to 110 ± 2.0% for NADH and 88 ± 3.0% to 95.4 ± 4.0% for EtOH. The results obtained were very consistent and did not differ considerably from the reported approaches at a 95% confidence limit. The possible mechanism of the CL reaction is also explained briefly.

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Quantitative analysis of intracellular coenzymes in Saccharomyces cerevisiae using ion pair reversed phase ultra high performance liquid chromatography tandem mass spectrometry

Cited by 55 publications

References 28 publications

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

Analytical Approaches for the Quantitation of Redox-active Pyridine Dinucleotides in Biological Matrices

13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea

Flow injection assays for NADH and ethanol using photosensitized rose bengal and luminol–copper (II) chemiluminescence system

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