Quantitative analysis of iridoids, secoiridoids, xanthones and xanthone glycosides in Gentiana lutea L. roots by RP-HPLC and LC–MS

Aberham, Anita; Schwaiger, Stefan; Stuppner, Hermann; Ganzera, Markus

doi:10.1016/j.jpba.2007.07.001

Cited by 90 publications

(66 citation statements)

References 9 publications

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“…Our results are in good agreement with levels of phenolic compounds measured previously in R. canina [9,28,29], R. rosea [25], and H. perforatum [11,31]. At the same time, terpenoids, but not phenolic compounds, are key constituents of G. lutea rhizomes [32,33,41].…”

Section: Total Antioxidant Capacity Of Plant Extracts Depends On Phensupporting

confidence: 91%

Effects of pH on antioxidant and prooxidant properties of common medicinal herbs

2016

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Background: We studied in vitro and vivo antioxidant and prooxidant abilities of aqueous extracts from Rosa canina L., Rhodiola rosea L., Hypericum perforatum L., and Gentiana lutea L. Methodology: Total antioxidant capacity was measured by four assays (phosphomolybdate method, Fe3+-reducing activity, ABTS•+ scavenging, H2O2 scavenging). Prooxidant activity was estimated by H2O2 production. Yeast viability in the presence of H2O2 and/or plant extracts was determined by plating or by counting live cells’ number. Results: Plant extracts differed in the total phenolic content (R. canina > R. rosea > H. perforatum > G. lutea) which clearly correlated with their ABTS•+ scavenging activity (R2 = 0.963). H2O2 scavenging activity was not clearly associated with plant phenol levels and was significantly higher in acidic, than in alkaline medium. In line with this, plant extracts effectively protected yeast S. cereviasiae against H2O2 and stimulated reproductive ability of yeast cells at acidic but not at alkaline pH. At alkaline pH, plant extracts produced certain amounts of H2O2 which were related to their phenolic content. Conclusion: The antioxidant activity of plant extracts is decreased at alkaline pH with an increase in the prooxidant activity. It reduces protective capacity of plant extracts against oxidative and other stresses in vivo.

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Section: Total Antioxidant Capacity Of Plant Extracts Depends On Phensupporting

confidence: 91%

Effects of pH on antioxidant and prooxidant properties of common medicinal herbs

2016

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“…Several HPLC methods have been developed for naturally occurring xanthones using microporous chemically bonded silica gel (Micropak CN column), solvent hexanechloroform (13 : 7, v/v), isooctane-CHCl 3 (3 : 17, v/v), or dioxane-dichloromethane (1 : 9) detected at 254 nm by UV detector [60]. Polar aglycones as well as glycosides of xanthones are also resolved on reversed phase column (C 8 and C 18 ) using acetonitrile-water as mobile phase [75,76]. High-speed counter current chromatography (HSCCC) and high performance centrifugal partition chromatography (HPCPC) were also used for the separation and isolation of mangiferin and neomangiferin from an extract of Anemarrhena asphodeloides [77] and -mangostins andmangostins from mangosteen pericarp, respectively [78].…”

Section: Methods For Isolation and Characterization Of Xanthonesmentioning

confidence: 99%

Naturally Occurring Xanthones: Chemistry and Biology

Negi

Bisht

Singh

et al. 2013

Journal of Applied Chemistry

164

120

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Xanthones are one of the biggest classes of compounds in natural product chemistry. A number of xanthones have been isolated from natural sources of higher plants, fungi, ferns, and lichens. They have gradually risen to great importance because of their medicinal properties. This review focuses on the types, isolation, characterization, biological applications, and biosynthesis of naturally occurring xanthones isolated so far. Different physicochemical and instrumental methods such as liquid-solid and liquid-liquid extraction, TLC, flash chromatography, column chromatography, IR,1H NMR and13C NMR spectroscopy, GLC, HPLC, GC, and LCMS have been widely used for isolation and structural elucidation of xanthones. Hepatoprotective, anticarcinogenic, antileprosy, antimalarial, antioxidant, anticholinergic, mutagenicity, radioprotective, immunomodulatory, antibone resorption, antiparasitic, neuraminidase inhibitory, anticomplement, antibacterial, antifungal, algicidal, anti-HIV, cardioprotective, antitumoral, antidiabetes, antihyperlipidemic, antiatherogenic, anti-inflammatory, antiulcer, antidiabetic, hypolipidemic, analgesic, antiasthmatic, antihistaminic, antiamoebic, diuretic, antidiarrheal, larvicidal, and ovicidal activities have been reported for natural occurring xanthones. To a certain extent, this review provides necessary foundation for further research and development of new medicines.

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“…All hairy root clones were analyzed together with a wild G. cruciata root sample (Bolu; Abant lake side, 1350 m) for content of relevant metabolites by a previously described HPLC method (Aberham et al 2007).…”

Section: Hplc Analysismentioning

confidence: 99%

“…Not only secoiridoids are relevant for the biological effects of gentian; other constituents ( Fig. 1), such as loganic acid, xanthone glycosides (gentioside and its isomer) and xanthones like gentisin and isogentisin are also biologically active (Aberham et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Induction of Gentiana cruciata hairy roots and their secondary metabolites

et al. 2011

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Gentiana cruciata L. (cross gentian) is a medicinal and ornamental plant. The root extracts of this species are known to exhibit many curative properties. The natural Gentiana populations are exposed to great danger because of their uncontrolled usage. In this study, hairy roots from Gentiana cruciata L. stem and leaf explants belonging to three different clones were induced by inoculation with four different Agrobacterium rhizogenes wild strains namely A4, 15834, 8196 and R1000. Induction of the root transformation was significantly dependent on the explant type used. On the other hand, the genotype and bacterial strain had no significant effect on hairy root formation. Hairy root formation percentages of the explants varied between 5.6-33.3% in the stem explants, and between 0.0-6.7% in the leaf explants. Transformations of the hairy roots were confirmed by PCR using rolC specific primers, and revealed the absence of contaminating A. rhizogenes with virC primers. Total of twelve hairy root clones were obtained, and their secondary metabolite content was also analyzed by HPLC. Quantitative results exhibited that gentiopicroside was the most abundant compound in all root samples. Furthermore, metabolites such as loganic acid, swertiamarin, and sweroside were also identified and quantified in the samples.

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Quantitative analysis of iridoids, secoiridoids, xanthones and xanthone glycosides in Gentiana lutea L. roots by RP-HPLC and LC–MS

Cited by 90 publications

References 9 publications

Effects of pH on antioxidant and prooxidant properties of common medicinal herbs

Effects of pH on antioxidant and prooxidant properties of common medicinal herbs

Naturally Occurring Xanthones: Chemistry and Biology

Induction of Gentiana cruciata hairy roots and their secondary metabolites

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