Quantitative Analysis of Mouse Retinal Layers Using Automated Segmentation of Spectral Domain Optical Coherence Tomography Images

Dysli, Chantal; Enzmann, Volker; Sznitman, Raphael; Zinkernagel, Martin

doi:10.1167/tvst.4.4.9

Cited by 79 publications

(86 citation statements)

References 31 publications

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“…This result supports previous studies, which have shown that macular inner retinal thicknesses were proportional to loss of peripapillary RNFL thickness and degree of glaucoma 18–23 .…”

Section: Discussionsupporting

confidence: 93%

Relationship between Retinal Inner Nuclear Layer Thickness and Severity of Visual Field Loss in Glaucoma

Kim

2017

Sci Rep

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Glaucoma is a disease characterized by pathologic changes in inner retinal layers, which are comprised of retinal ganglion cells (RGCs). As retinal ganglion cells (RGCs) cross over other retinal neurons that are connected by synapses, it is meaningful to investigate the outer retinal changes in glaucoma. We evaluated the association between thicknesses of segmented retinal layers in macular region and severity of visual field loss in open-angle glaucoma (OAG). This study involved 103 glaucomatous eyes. Retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), and outer nuclear layer (ONL) thicknesses were measured at the macular level using the Spectral-domain optical coherence tomography with segmentation software. The functional losses were measured using 24-2 standard automated perimetry. Macular structure losses were positively correlated with functional loss for RNFL, GCL, and IPL (R = 0.550, 0.637, and 0.649, respectively, P < 0.001) and negatively correlated with INL (R = −0.295, P = 0.041). By multivariate regression analysis, INL thickness was significantly associated with visual field mean deviation (dB) and optic disc hemorrhage. These finding carefully suggest reactive responses of neuronal or glial cells located in the INL occur during glaucoma progression.

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“…This result supports previous studies, which have shown that macular inner retinal thicknesses were proportional to loss of peripapillary RNFL thickness and degree of glaucoma 18–23 .…”

Section: Discussionsupporting

confidence: 93%

Relationship between Retinal Inner Nuclear Layer Thickness and Severity of Visual Field Loss in Glaucoma

Kim

2017

Sci Rep

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“…54 Obviously, differences in routes of drug administration (intravitreal injection in chickens and intraperitoneal injection in our study) lead to different drug distributions within the retina. During FDM development, the unchanged dopamine level in mice 12,13,36 is also different from the reduced dopamine level in chickens. 6,11 However, with respect to the active status of dopamine receptors, which is the most important point while considering the receptor mechanism of APO activity, genetic KO provides an alternative approach that is much more complete and persistent than pharmacologic intervention.…”

Section: D1r-dependent Mechanism Of Apomorphine In Myopiamentioning

confidence: 86%

“…The thickness of individual retinal layers was quantified using a standard Early Treatment Diabetic Retinopathy Study grid, which was centered manually on the ONH. 36 Mean values of the outer ring (corresponding to a distance of 600 lm from the center of the ONH, approximately 178) were analyzed by an experienced annotator using the Heidelberg system. The measurements included thicknesses of the retinal nerve fiber layer (RNFL), ganglion cell layer to inner plexiform layer (GCLþIPL), inner nuclear layer to outer plexiform layer (INLþOPL), outer nuclear layer (ONL), photoreceptor layer (PR), and total retinal thickness.…”

Section: Retinal Layer Thickness Measurementsmentioning

confidence: 99%

Dopamine D1 Receptors Contribute Critically to the Apomorphine-Induced Inhibition of Form-Deprivation Myopia in Mice

Huang

Zhang

Wang

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To determine the roles of dopamine D2 receptors (D2Rs) and dopamine D1 receptors (D1Rs) in the inhibition of form-deprivation myopia (FDM) by the nonselective dopamine agonist apomorphine (APO) in D2R-knockout (D2R-KO) and D1R-KO mice. METHODS.Retinal layer thicknesses and electroretinograms (ERGs) were analyzed in KO mice and in D2R and D1R antagonist-treated mice. D2R-KO or D1R-KO mice and wild-type (WT) littermates were subjected to form deprivation during postnatal weeks 5 to 8. Both groups were intraperitoneally injected daily with either APO (5 lg/g body weight) dissolved in 1 lg/ lL ascorbic acid or vehicle alone. Refraction, vitreous chamber depth (VCD), and axial length (AL), among other parameters, were measured prior to and at the end of the treatment period.RESULTS. The retinal layer thicknesses and ERGs in KO mice were similar to those treated with D2R and D1R antagonists. APO administration in WT mice inhibited the development of FDM by approximately 80%. FDM in D2R-KO mice was inhibited approximately 50% compared with WT mice and was further inhibited by APO to a level similar to that in APO-treated WT mice. FDM development in D1R-KO mice was similar to that in WT mice and was not affected by APO administration. The changes in VCD and AL were consistent with refraction data.CONCLUSIONS. In mice, APO-mediated FDM inhibition was abolished by D1R KO but not D2R KO. This indicates the specificity of D1Rs for the pharmacologic inhibitory effect of APO on FDM and a nonessential role of D2Rs in this process in mice.

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“…The automated retinal segmentation algorithm adopted by SD-OCT (SPECTRALIS, Heidelberg Engineering, Germany) has been used on mice. [13] However, to our knowledge, this study is the first report to differentiate the OPL and ONL layers automatically by the OCT program itself, and carry out segmentation with thickness and volume into 9 retinal layers in human. In several previous studies, the thicknesses of the RNFL, GCL, IPL, and INL were so thin that they could not be reliably measured, [14] and the segmentation of the macular layer into 6 or 7 retinal layers (OPL+ONL as 1 or lack of an RPE layer), rather than 9 individual layers, was performed.…”

Section: Discussionmentioning

confidence: 99%

Effect of age and sex on retinal layer thickness and volume in normal eyes

2016

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The aim of the study was to evaluate the effect of sex and age on the thickness of the retinal layer in normal eyes using spectral-domain optical coherence tomography (SD-OCT).Fifty healthy subjects between the ages of 20 and 80 had their retinal layers measured using SD-OCT at Seoul St. Mary's Hospital. Mean thickness and volume were measured for 9 retinal layers in the fovea, the pericentral ring, and the peripheral ring. The differences of sex- and age-related thickness and volume in each retinal layer were analyzed.The retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), and outer plexiform layer (OPL) were thinnest in the fovea area, whereas the outer nuclear layer (ONL), photoreceptor layer (PHL), and retinal pigment epithelium (RPE) were thickest at similar locations. Mean thickness of the RNFL, GCL, IPL, and OPL was significantly greater in men than women. However, mean thickness of the ONL was greater in women than in men. When compared between patients < 30 years and > 60 years of age, the thickness and volume of peripheral RNFL, GCL, and pericentral and peripheral IPL were significantly larger in the younger group than the older group. Conversely, the thickness and volume of foveal INL and IR were larger in the older group than in the younger group.The thickness and volume of the retinal layer in normal eyes significantly vary depending on age and sex. These results should be considered when evaluating layer analysis in retinal disease.

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Quantitative Analysis of Mouse Retinal Layers Using Automated Segmentation of Spectral Domain Optical Coherence Tomography Images

Cited by 79 publications

References 31 publications

Relationship between Retinal Inner Nuclear Layer Thickness and Severity of Visual Field Loss in Glaucoma

Relationship between Retinal Inner Nuclear Layer Thickness and Severity of Visual Field Loss in Glaucoma

Dopamine D1 Receptors Contribute Critically to the Apomorphine-Induced Inhibition of Form-Deprivation Myopia in Mice

Effect of age and sex on retinal layer thickness and volume in normal eyes

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