Quantitative analysis of mutant p53 protein in breast tumor cytosols and study of its association with other biochemical prognostic indicators in breast cancer

Abstract

Breast tumors are thought to originate, grow, and metastasize in an environment which includes steroid hormone receptors, their cognate steroid ligands, and many gene products which are regulated by steroid hormone receptor-ligand complexes. In this paper we describe highly sensitive and quantitative immunofluorometric procedures for measuring three proteins that are candidate prognostic indicators in breast cancer, namely, the p53 tumor suppressor gene product, carcinoembryonic antigen (CEA), and prostate specific antigen (PSA). These proteins were quantified in over 950 cytosolic tumor extracts along with estrogen and progesterone receptors (ER, PR). Association analysis between all five biochemical parameters revealed strong negative associations between p53 and receptors and strong positive associations between CEA and receptors. Negative associations between p53 and CEA and between CEA and PSA were also found. These associations, not quantitatively studied in previous reports, are related to each other using a hypothetical model. The observed associations may further contribute to the understanding of the biology of breast tumors.

P53 Accumulation in Primary Breast Cancer: A Comparison between Immunohistochemistry and a Novel Luminometric Immunoassay

“…Moreover, in conformity with findings from other studies, we did not observe any correlation between LIA p53 values and nodal status or hormone receptor status, whereas we did detect a relation with various biologic features, the LIA p53 content being higher in large rapidly proliferating tumors than in small slowly proliferating tumors [13,15].…”

A novel quantitative immunoassay system for p53 using antibodies selected for optimum designation of p53 status.

“…This approach permitted the detection of 0.1 ng/ml of p53 in an enriched recombinant p53 preparation. This is less than that described by other assays using CM1.9 10 We are, however, confident in suggesting that the analyte measured in our system is p53.…”

Tissue carcinoembryonic antigen and oestrogen receptor status in breast carcinoma: an immunohistochemical study of clinical outcome in a series of 252 patients with long-term follow-up

“…This is in keeping with the study of Sundblad et al (1996), but is at variance with the study performed by Esteban et al (1994), which reported an association between CEA expression and positive ER status. A positive association between CEA and ER status has been found by other studies that used different CEA antibodies and/or different methods to identify CEA expression, such as immunoradiometric (Gion et al, 1986) or immunofluorimetric (Levesque et al, 1994) procedures on cytosolic tumour extracts.…”