Quantitative Analysis of Petasin and Pyrrolizidine Alkaloids in Leaves and Rhizomes of<i>in situ</i>Grown<i>Petasites hybridus</i>Plants

Wildi, Eckhart; Langer, Theodor; Schaffner, W.; Büter, Karin Berger

doi:10.1055/s-2006-957422

Cited by 32 publications

(29 citation statements)

References 3 publications

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“…Th e lowest amount of total PAs was found in Petasites hybridus extract (59.61 ± 0.22 μg/g dry mass). Th e results of the spectrophotometric assay are consistent with the ones found in the literature [5,12,13].…”

Section: Discussionsupporting

confidence: 90%

Phytotoxicity Assessment of Certain Phytochemical Products Containing Pyrrolizidine Alkaloids

Cristina

Ilie

et al. 2013

Acta Medica Marisiensis

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Introduction: Tussilago farfara (coltsfoot), Petasites hybridus (common butterbur), Senecio vernalis (eastern groundsel) and Symphytum offi cinale (comfrey) are species traditionally used in phytotherapy that besides the therapeutic compounds contain toxic pyrrolizidine alkaloids (PAs). The aim of the paper is to determine the total PAs content and the phytotoxicity of the above species. Material and methods:The quantitative determination of pyrrolizidine alkaloids is based on the stoichiometric reaction of protonated alkaloids with methyl orange. In acidic conditions the dye is released from the complex and its color is assessed spectrophotometrically using a linear regression curve of senecionine as a standard. The phytotoxicity was assessed by Triticum bioassay that studies the effect of the extracts (0.001-5.00%, w/v) upon root elongation (inhibitory concentration -IC50) and on the karyokinetic fi lm. Results: The highest amount of total PAs was found in Senecio vernalis (654.8 ± 35.96 μg/g dry plant) and the lowest in Petasites hybridus. The lowest IC50 was found for Tussilago farfara followed by Petasites hybridus, Senecio vernalis, and Symphytum offi cinale. The results were supported by microscopic examination. Conclusions:The results of the spectrophotometric assay are consistent with the ones found in the literature. All extracts inhibited the elongation of the main root of wheat caryopses, however, no correlation between phytotoxicity and the PAs concentration could be emphasized.

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Section: Discussionsupporting

confidence: 90%

Phytotoxicity Assessment of Certain Phytochemical Products Containing Pyrrolizidine Alkaloids

Cristina

Ilie

et al. 2013

Acta Medica Marisiensis

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“…These literatures [19] and our results provide useful analysis method for determination of bekkenolide B. Wildi et al [20] conducted the analyses of petasin distribution in rhizomes and leaves of the plant. The mean petasin content of populations ranged from 7.4 to 15.3 mg/g dry weight in rhizomes and from 3.3 to 11.4 mg/g dry weight in leaves of P. hybridus.…”

Section: Structure Elucidation Of Bakkenolide Bmentioning

confidence: 56%

“…4) and petasin rich extracts [8,20] since the changes in petasin concentrations between leaves and rhizomes of the plant are not tremendous [3]. …”

Section: Structure Elucidation Of Bakkenolide Bmentioning

confidence: 99%

Isolation, Quality Evaluation, and Seasonal Changes of Bakkenolide B in Petasites japonicus by HPLC

Kim¹,

Kim²,

Jung³

et al. 2014

Journal of Life Science

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The leaves of Peatasites japonicus are a traditional oriental medicine with diverse biological activities.A simple and specific analytical method for the quantitative determination of bakkenolide B constituents from methanolic extract of the leaves of P. japonicus was developed. Bakkenolide B was isolated from the leaves of P. japonicus, and its structure was elucidated based on 1D, 2D NMR, and GC-MS spectral data. A liquid chromatographic method was developed to evaluate the quality of P. japonicus through determination of major active compound, bakkenolide B. The wavelengths at 254 and 215 nm were chosen to determine bakkenolide B. The recovery of the method was in the range of 98.6 to 103.1%, and bakkenolide B showed good linearity (r 2 =0.999) within test ranges. The developed method was applied to the determination of bakkenolide B in the plant part and seasonal changes. The results showed that the content of bakkenolide B in the leaf was higher than in the petiole and rhizome. In this study, a simple, rapid, and reliable high-performance liquid chromatography method was used to determine the percentage and composition of bakkenolide B in P. japonicus procured from different Petasites species plants in South Korea. The method can be employed in routine quantitative analysis and quality control of different products in the market.

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“…17) Among these, the retronecine type, including senecionine and integerrmine, are reported to be major pyrrolizidine alkaloids in butterbur species, 33,34) although at low levels. 19) Pyrrolizidine alkaloids usually do not have toxic effects per se, but the formation of reactive pyrrolic ester metabolites catalyzed by cytochrome P-450 monooxygenase is primarily responsible for hepatotoxicity and carcinogenicity in experimental animals. 13) Despite similar structures, it has been observed that differing CYP modulation is dependent on the type of pyrrolizidine alkaloid present.…”

Section: Discussionmentioning

confidence: 99%

“…17) Hence, some researchers have suggested that unpurified butterbur or extracts can be hepatotoxic and carcinogenic in livestock. 9,16,18) Recently, Wildi and colleagues 19) suggested that the leaves of P. hybridus contain a negligible level of toxicants and are clearly a good source for drug. To date, however, there have been few reports on evaluation of the safety of Japanese butterbur leaves.…”

mentioning

confidence: 99%

Japanese Butterbur (Petasites japonicus) Leaves Increase Hepatic Oxidative Stress in Male Rats

Han¹,

Sekikawa²,

Shimada³

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Quantitative Analysis of Petasin and Pyrrolizidine Alkaloids in Leaves and Rhizomes ofin situGrownPetasites hybridusPlants

Cited by 32 publications

References 3 publications

Phytotoxicity Assessment of Certain Phytochemical Products Containing Pyrrolizidine Alkaloids

Phytotoxicity Assessment of Certain Phytochemical Products Containing Pyrrolizidine Alkaloids

Isolation, Quality Evaluation, and Seasonal Changes of Bakkenolide B in Petasites japonicus by HPLC

Japanese Butterbur (Petasites japonicus) Leaves Increase Hepatic Oxidative Stress in Male Rats

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