Quantitative analysis of proteome dynamics in a mouse model of asthma

Funke, Lukas; Gnipp, Stefanie; Ahrens, Maike; Eisenacher, Martin; Peters, Marcus; Sitek, Barbara; Bracht, Thilo

doi:10.1111/cea.13843

Cited by 2 publications

(3 citation statements)

References 33 publications

(32 reference statements)

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“…Recent applications range from the quantification of one single protein in bronchoalveolar lavage, 120 to high‐throughput protein identification in tear fluid, 121 skin swabs, 122 sputum, 123 serum, 124 , 125 , 126 , 127 or even mouse lungs. 128 In addition, proteomics methods were applied to identify immunoreactive proteins, 129 , 130 , 131 or changes in N‐glycan modification patterns. 132 With this broad spectrum of potential applications, it is expected that proteomics results will be central to many future research studies in the allergy field.…”

Section: Basic Omics Strategiesmentioning

confidence: 99%

“…The considerable improvements regarding sensitivity, applicability, and versatility of proteomics technologies have led to their wider application also in the field of allergy (Table ). Recent applications range from the quantification of one single protein in bronchoalveolar lavage, 120 to high‐throughput protein identification in tear fluid, 121 skin swabs, 122 sputum, 123 serum, 124–127 or even mouse lungs 128 . In addition, proteomics methods were applied to identify immunoreactive proteins, 129–131 or changes in N‐glycan modification patterns 132 .…”

Section: Basic Omics Strategiesmentioning

confidence: 99%

“…Long noncoding RNAs from RNAsequencing data analysis 203 Proteomics High-throughput protein identification, labelfree quantification 121,122,126,128 Data analysis using similarity network fusion 125 Allergen characterization [129][130][131] N-glycan characterization 132 Single protein quantification 120…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Omics technologies in allergy and asthma research: An EAACI position paper

Radzikowska

Baerenfaller

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et al. 2022

Allergy

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Allergic diseases and asthma are heterogenous chronic inflammatory conditions with several distinct complex endotypes. Both environmental and genetic factors can influence the development and progression of allergy. Complex pathogenetic pathways observed in allergic disorders present a challenge in patient management and successful targeted treatment strategies. The increasing availability of high-throughput omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics allows studying biochemical systems and pathophysiological processes underlying allergic responses. Additionally, omics techniques present clinical These different cellular components are tightly interconnected with TA B L E 1 Summary of methods, protocols, and data analysis pipelines most frequently used in omics approaches for allergic diseases research Methods and protocols Data analysis pipeline Mechanisms of allergic diseases Genomics and transcriptomics Microarray 181,182GWAS 183 and RNA microarray bioinformatics 184,185

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Section: Basic Omics Strategiesmentioning

confidence: 99%

Section: Basic Omics Strategiesmentioning

confidence: 99%

See 1 more Smart Citation

Omics technologies in allergy and asthma research: An EAACI position paper

Radzikowska

Baerenfaller

Cornejo-García

et al. 2022

Allergy

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Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis

et al. 2022

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Asthma is a heterogeneous pulmonary disorder, the progression and chronization of which leads to airway remodeling and fibrogenesis. To understand the molecular mechanisms of pulmonary fibrosis development, key genes forming the asthma-specific regulome and involved in lung fibrosis formation were revealed using a comprehensive bioinformatics analysis. The bioinformatics data were validated using a murine model of ovalbumin (OVA)-induced asthma and post-asthmatic fibrosis. The performed analysis revealed a range of well-known pro-fibrotic markers (Cat, Ccl2, Ccl4, Ccr2, Col1a1, Cxcl12, Igf1, Muc5ac/Muc5b, Spp1, Timp1) and a set of novel genes (C3, C3ar1, Col4a1, Col4a2, Cyp2e1, Fn1, Thbs1, Tyrobp) mediating fibrotic changes in lungs already at the stage of acute/subacute asthma-driven inflammation. The validation of genes related to non-allergic bleomycin-induced pulmonary fibrosis on asthmatic/fibrotic lungs allowed us to identify new universal genes (Col4a1 and Col4a2) associated with the development of lung fibrosis regardless of its etiology. The similarities revealed in the expression profiles of nodal fibrotic genes between asthma-driven fibrosis in mice and nascent idiopathic pulmonary fibrosis in humans suggest a tight association of identified genes with the early stages of airway remodeling and can be considered as promising predictors and early markers of pulmonary fibrosis.

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Quantitative analysis of proteome dynamics in a mouse model of asthma

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 2 publications

References 33 publications

Omics technologies in allergy and asthma research: An EAACI position paper

Omics technologies in allergy and asthma research: An EAACI position paper

Asthma and Post-Asthmatic Fibrosis: A Search for New Promising Molecular Markers of Transition from Acute Inflammation to Pulmonary Fibrosis

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