Quantitative analysis of Sertoli cells in neonatally oestrogen-treated rats

Gaytan, F.; Aguilar, E.

doi:10.1530/jrf.0.0790589

Cited by 10 publications

(5 citation statements)

References 28 publications

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“…9). Although changes in testicular morphology and function after neonatal exposure to estrogenic compounds have been well-characterized [10,11,35,45], little attention has been paid to molecular events in the rat testis after prenatal estronization. We examined the changes of testicular expression of ERα and β genes in this experimental model.…”

Section: Discussionmentioning

confidence: 99%

Decreased Sperm Number and Motile Activity on the F1 Offspring Maternally Exposed to Butyl p-Hydroxybenzoic Acid(Butyl Paraben).

et al. 2002

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ABSTRACT. Butyl p-hydroxybenzoic acid (butyl paraben, BP) is widely used as a preservative in food and cosmetic products. Routledge et al showed that BP is weakly estrogenic in both in vitro and in vivo (rat uterotrophic) analyses. We investigated whether maternal exposures to BP during gestation and lactation periods affected the development of the reproductive organs of the F1 offspring. Pregnant Sprague-Dawley rats were injected subcutaneously with 100 or 200 mg/kg of BP from gestation day (GD) 6 to postnatal day (PND) 20. In the group exposed to 200 mg/kg of BP, the proportion of pups born alive and the proportion of pups surviving to weaning were decreased. The body weights of female offspring were significantly decreased at PND 49. The weights of testes, seminal vesicles and prostate glands were significantly decreased in rats exposed to 100 mg/kg of BP on PND 49. In contrast, the weights of female reproductive organs were not affected by BP. The sperm count and the sperm motile activity in the epididymis were significantly decreased at doses of 100 and 200 mg/kg of BP. In accordance with the sperm count in the epididymis, the number of round spermatids and elongated spermatids in the seminiferous tubule (stage VII) were significantly decreased by BP. Testicular expression of estrogen receptor (ER)-α and ER-β mRNA was significantly increased in 200 mg/kg of BP treated group at PND 90. Taken together, these results indicated that maternal exposure of BP might have adverse effects on the F1 male offspring.KEY WORDS: butyl p-hydroxybenzoic acid, male reproductive organ, maternal exposure, sperm.

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Section: Discussionmentioning

confidence: 99%

Decreased Sperm Number and Motile Activity on the F1 Offspring Maternally Exposed to Butyl p-Hydroxybenzoic Acid(Butyl Paraben).

et al. 2002

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“…Adult male rats treated with oestradiol on the first day of life show atrophy of the testes and sexual accessory glands (Kind, Folch, Maqueo et al 1965; Aguilar, Tejero, Vaticón & Fernandez-Galaz, 1984), impaired maturation of germ cells (Dhar & Setty, 1976; and of Sertoli and Leydig cells (Gaytan, Pinilla, Aguilar et al 1986;Gaytan & Aguilar, 1987) and decreased serum testosterone concentrations (Frick, Chang & Kind, 1969;Pinilla, Cocconi, Zoppi & Martini, 1989).…”

Section: Introductionmentioning

confidence: 99%

Hypothalamic-pituitary function in neonatally oestrogen-treated male rats

Pinilla

Garnelo²,

Gaytan³

et al. 1992

Journal of Endocrinology

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Neonatal oestrogen administration to male rats permanently impaired the function of the pituitary-testicular axis possibly by inhibiting neonatal gonadotrophin secretion. To analyse the hypothalamus and/or pituitary involvement in this inhibition, pituitary responsiveness to acute stimulation with LH-releasing hormone (LHRH) was studied in vivo and in vitro in Wistar male rats injected on day 1 of age with oestradiol benzoate (OB) or olive oil. FSH and LH pituitary content and plasma concentrations were reduced in oestrogenized male rats at days 10 and 16 of age. Likewise, the in-vivo increase in gonadotrophin plasma concentrations after acute stimulation with LHRH was almost completely suppressed in 10- and 16-day-old oestrogenized males. In vitro, the increased secretion of FSH after LHRH stimulation was abolished and the LH response strongly reduced in pituitaries from oestrogenized males. Finally, the effects of neonatal oestrogenization were not abolished by treatment from day 1 to day 15 with an LHRH agonist (0.01 microgram/kg per 12 h). We conclude that in male rats the effects of oestrogenization are due to both a reduction in LHRH endogenous secretion and a decrease in the pituitary responsiveness to LHRH.

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“…Sharpe et al (1998) showed in their study that the number of Sertoli cells per testis was reduced by 40%. On the other hand, there are reports showing that the number was unchanged (Gaytan et al, 1986;Gaytan and Aguilar, 1987). In their studies, Gaytan and colleagues treated day 1 male rats with a single administration of 500 g of estradiol benzoate per rat.…”

Section: Discussionmentioning

confidence: 99%

Neonatally Administered Diethylstilbestrol Retards the Development of the Blood‐Testis Barrier in the Rat

TOYAMA,

OHKAWA,

OKU

et al. 2001

Journal of Andrology

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Newborn rats were treated with 10 μg of diethylstilbestrol (DES) on alternate days from the 2nd to the 12th postnatal day, and the testes were sequentially examined up to 105 days of age by light, electron, and confocal laser microscopy. In control rats, spermatozoa and step 19 spermatids were observed in stage VIII seminiferous tubules at 56 days of age. Spermatogenic cells in DES‐treated rats differentiated normally from birth until 21 days of age, after which differentiation continued only to the pachytene‐spermatocyte stage. From this age onward, spermatogenic cells older than pachytene spermatocytes were not found until 56 days of age. After this point, the cells resumed differentiation and finally became spermatozoa by 91 days of age; that is, 35 days later than control rats. Electron and confocal laser microscopy showed that in the normal rat, the formation of the ectoplasmic specialization between adjoining Sertoli cells was observed as early as 20 days of age. In contrast, the specialization was not formed until 56 days of age in DES‐treated rats. Furthermore, the delay in functional maturation of this structure as the blood‐testis barrier was confirmed by intercellular tracer experiments. It is clear that neonatal administration of DES delayed the establishment of the blood‐testis barrier for 4 weeks. Consequently, during this period, pachytene spermatocytes were exfoliated from the seminiferous epithelium without completion of meiosis.

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Quantitative analysis of Sertoli cells in neonatally oestrogen-treated rats

Cited by 10 publications

References 28 publications

Decreased Sperm Number and Motile Activity on the F1 Offspring Maternally Exposed to Butyl p-Hydroxybenzoic Acid(Butyl Paraben).

Decreased Sperm Number and Motile Activity on the F1 Offspring Maternally Exposed to Butyl p-Hydroxybenzoic Acid(Butyl Paraben).

Hypothalamic-pituitary function in neonatally oestrogen-treated male rats

Neonatally Administered Diethylstilbestrol Retards the Development of the Blood‐Testis Barrier in the Rat

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