Quantitative analysis of sesquiterpene lactones in extract of Arnica montana L. by 1H NMR spectroscopy

Staneva, Jordanka; Denkova, Pavletta; Todorova, Milka; Evstatieva, L.

doi:10.1016/j.jpba.2010.08.018

Cited by 51 publications

(38 citation statements)

References 12 publications

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“…Since the mean molecular weight of Arnica m . sesquiterpene lactones is 340 g/mol [31], this amount is equivalent to 1.05 x 10 −3 Mol/L and the calculated concentration of these active substances is 1.05 x 10 −5 Mol/L in Arnica m . 1c and 1.05 x 10 −7 Mol/L in Arnica m .…”

Section: Resultsmentioning

confidence: 99%

Arnica montana Stimulates Extracellular Matrix Gene Expression in a Macrophage Cell Line Differentiated to Wound-Healing Phenotype

et al. 2016

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Arnica montana (Arnica m.) is used for its purported anti-inflammatory and tissue healing actions after trauma, bruises, or tissue injuries, but its cellular and molecular mechanisms are largely unknown. This work tested Arnica m. effects on gene expression using an in vitro model of macrophages polarized towards a “wound-healing” phenotype. The monocyte-macrophage human THP-1 cell line was cultured and differentiated with phorbol-myristate acetate and Interleukin-4, then exposed for 24h to Arnica m. centesimal (c) dilutions 2c, 3c, 5c, 9c, 15c or Control. Total RNA was isolated and cDNA libraries were sequenced with a NextSeq500 sequencer. Genes with significantly positive (up-regulated) or negative (down-regulated) fold changes were defined as differentially expressed genes (DEGs). A total of 20 DEGs were identified in Arnica m. 2c treated cells. Of these, 7 genes were up-regulated and 13 were down-regulated. The most significantly up-regulated function concerned 4 genes with a conserved site of epidermal growth factor-like region (p<0.001) and three genes of proteinaceous extracellular matrix, including heparin sulphate proteoglycan 2 (HSPG2), fibrillin 2 (FBN2), and fibronectin (FN1) (p<0.01). Protein assay confirmed a statistically significant increase of fibronectin production (p<0.05). The down-regulated transcripts derived from mitochondrial genes coding for some components of electron transport chain. The same groups of genes were also regulated by increasing dilutions of Arnica m. (3c, 5c, 9c, 15c), although with a lower effect size. We further tested the healing potential of Arnica m. 2c in a scratch model of wound closure based on the motility of bone marrow-derived macrophages and found evidence of an accelerating effect on cell migration in this system. The results of this work, taken together, provide new insights into the action of Arnica m. in tissue healing and repair, and identify extracellular matrix regulation by macrophages as a therapeutic target.

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Section: Resultsmentioning

confidence: 99%

Arnica montana Stimulates Extracellular Matrix Gene Expression in a Macrophage Cell Line Differentiated to Wound-Healing Phenotype

et al. 2016

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“…In particular, quantification using 1 H NMR (qHNMR) is widely applied in the quantification of medicines [11,12], beverage components [13,14], and natural products in medicinal plant extracts [15][16][17] due to its high sensitivity and the widespread presence of 1 H nuclei in organic molecules [18]. In qHNMR, the content or concentration of the analyte is determined using the ratio between the integral values of a specific signal of the analyte to that of an internal standard (IS).…”

Section: Introductionmentioning

confidence: 99%

Quantification of acesulfame potassium in processed foods by quantitative 1H NMR

Ohtsuki

Sato

Abe

et al. 2015

Talanta

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“…NMR can also be used as a quantification tool, because of the fact that the area of integrated signal peak is directly proportional to the number of nuclei contributing to the signal. Since its first application in 1963, quantitative NMR (qNMR) has been widely applied in pharmaceutical and food industry . Some advantages of qNMR have been recognized, such as fast acquisition, nondestructive nature, simple sample preparation and handling, and the ability to measure an analyte in a mixture …”

Section: Introductionmentioning

confidence: 99%

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

Zhang

Oyler

et al. 2014

Journal of Pharmaceutical Sciences

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In vitro drug release evaluation is a very important step toward the quality control of nano- or micro-particular drug delivery systems. However, most quantitative techniques such as HPLC requires a dialysis membrane to separate the released free drug from these delivery systems, thus are not capable of direct detection and real-time quantification of the drug release. This study describes for the first time, a rapid, specific, and direct method for the real-time quantification of in vitro tenofovir (TNF) release from pH-sensitive microparticles using a Varian 400 MHZ 1H nuclear magnetic resonance (1H-NMR) spectrometer. Various analytical performance parameters such as linearity, precision, accuracy, limit of quantification (LOQ), limit of detection (LOD), and robustness were validated according to International Conference on Harmonization (ICH) guidelines. The in vitro release of TNF from microparticles in both simulated vaginal fluid (VFS) and the mixture (VSFS) of VFS and simulated semen fluid (SFS) was monitored and quantified in real-time using 1H-NMR. The capability of real-time quantification of in vitro drug release from microparticles not only provides a more accurate prediction of its biological behavior in vivo, but is also independent of potential interference from the dialysis membrane.

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Quantitative analysis of sesquiterpene lactones in extract of Arnica montana L. by 1H NMR spectroscopy

Cited by 51 publications

References 12 publications

Arnica montana Stimulates Extracellular Matrix Gene Expression in a Macrophage Cell Line Differentiated to Wound-Healing Phenotype

Arnica montana Stimulates Extracellular Matrix Gene Expression in a Macrophage Cell Line Differentiated to Wound-Healing Phenotype

Quantification of acesulfame potassium in processed foods by quantitative 1H NMR

Direct and Real-Time Quantification of Tenofovir Release from pH-Sensitive Microparticles into Simulated Biological Fluids Using 1H Nuclear Magnetic Resonance

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