Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy

Kanaya, K.; Muranaka, Yoshinori; Yonehara, Katsuhisa; Adachi, Kôichi

doi:10.1016/0739-6260(92)90072-l

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…After the fixative had been replaced with PBS, the specimens were dehydrated in graded ethanol and dried in a liquid CO 2 freeze-drying device (JFD-300, JEOL). Each segment was etched by the ion-beam bombardment method to allow visual inspection 12 and was processed further for scanning electron microscopy.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Photochemically Induced Endothelial Injury in the Mouse as a Screening Model for Inhibitors of Vascular Intimal Thickening

Kikuchi

Umemura

Kondo

et al. 1998

ATVB

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Abstract-We have established a mouse model of intimal thickening and assessed its suitability for experimental studies of intimal thickening. Neointimal formation was observed after endothelial injury by photochemical reaction between transluminal green light and systemically administered rose Bengal, which represents a nonmechanical approach to vessel wall denudation. Intimal thickening began 7 days after endothelial injury, reached a maximum after 21 days, and then remained unchanged for as long as 42 days. Furthermore, as a consequence of neointimal proliferation, the luminal area gradually decreased. The cells in the neointimal layer were identified as smooth muscle cells by immunohistochemical staining with an ␣-actin-specific antibody. Extracellular matrix deposition in the neointima was markedly increased beyond 14 days after injury. Smooth muscle cell proliferation, as measured by pulse labeling of 5-bromo-2Ј-deoxyuridine, was identified initially in the media 2 days after vessel wall denudation, with the proliferative activity's shifting almost exclusively to the neointima within 7 days. Endothelial regeneration, as indicated by Evans blue staining, was complete within 21 days after injury. To assess the suitability of this model for experimental studies on intimal thickening, the effect of tranilast, an antiallergy drug with a broad spectrum of pharmacological actions on intimal thickening, was investigated. Tranilast (100 mg ⅐ kg Ϫ1 ⅐ d Ϫ1 PO) significantly (PϽ0.05) reduced smooth muscle cell proliferation in the neointima and media 7 days after injury and neointimal formation 21 days after injury in treated mice compared with vehicle-treated mice. This simple experimental mouse model is suitable for studying factors promoting or inhibiting intimal thickening after endothelial injury and for developing therapeutic strategies against intimal thickening.

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Section: Scanning Electron Microscopymentioning

confidence: 99%

Photochemically Induced Endothelial Injury in the Mouse as a Screening Model for Inhibitors of Vascular Intimal Thickening

Kikuchi

Umemura

Kondo

et al. 1998

ATVB

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“…Ion milling is routinely used in many microscopy-related applications, such as transmission electron microscopy (TEM) specimen thinning, surface cleaning for Auger analysis and in secondary ion mass spectroscopy (SIMS). Ion beam techniques have been used for the removal of surface layers from biological material to reveal underlying features (Kanaya et al, 1982(Kanaya et al, , 1992aYonehara et al, 1989). These ,studies generally use an ion beam in the micrometres to millimetres diameter range, extracted from a gas discharge (Brown, 1989), and etch relatively large areas of the specimen.…”

Section: Focused Ion Millingmentioning

confidence: 99%

An application of scanned focused ion beam milling to studies on the internal morphology of small arthropods

Young¹,

Dingle²,

Robinson

et al. 1993

Journal of Microscopy

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Summary For the first time a scanned focused ion beam of approximately 50 nm diameter has been used to prepare biological material. Small defined areas of the surface were removed by ion etching to allow examination of the underlying structures with a scanning electron microscope. Different milling procedures were carried out on two anatomical features in mites of the genus Halarachne (Halarachnidae: Mesostigmata). In the first, square holes were milled into the surface of the peritrematal plate to reveal the structure of the underlying respiratory peritrematal groove. In the second, transverse cuts were made across the shafts of the sensory sensilli which make up the sensory Haller's organ on tarsus I. This latter procedure revealed detail both within the core and walls of sensilli. Details of specimen preparation and milling procedures, as well as suitability and interpretation of results, are presented.

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Observations on charging effects of non-conductive and uncoated materials by ion beam pre-bombardment in scanning electron microscopy

Kanaya

Ichise

Adachi

et al. 1992

Micron and Microscopica Acta

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Quantitative analysis of sputtering due to ion beam bombardment of solids and biological specimens in high resolution electron microscopy

Cited by 4 publications

References 17 publications

Photochemically Induced Endothelial Injury in the Mouse as a Screening Model for Inhibitors of Vascular Intimal Thickening

Photochemically Induced Endothelial Injury in the Mouse as a Screening Model for Inhibitors of Vascular Intimal Thickening

An application of scanned focused ion beam milling to studies on the internal morphology of small arthropods

Observations on charging effects of non-conductive and uncoated materials by ion beam pre-bombardment in scanning electron microscopy

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